LOCUS       sequence-260376-        1133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of GFP (as control) tagged with N-terminal
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1133)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-GFP_IDG-K
FEATURES             Location/Qualifiers
     source          1..1133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      114..123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             123..146
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    156..180
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(201..203,204..206,207..917)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(246..267)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(507..526)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     854..875
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(937..961)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gaatttgggg cattagcaga gctcgtttag tgaccgtcag atcgcctgga gacgccatcc
       61 acgctgtttt gacctccata gaagacaccg gcggccatac cggtcagctc gaggccacca
      121 tggactacaa ggacgacgac gacaagggcg gatcaacaag tttgtacaaa aaagcaggct
      181 ccgcggccgc ccccttcacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      241 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tctggcgagg
      301 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      361 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
      421 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      481 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      541 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      601 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
      661 tggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacatcgagg
      721 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      781 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
      841 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      901 tggacgagct gtacaagtcc aagggtgggc gcgccgaccc agctttcttg tacaaagtgg
      961 ttgattaaga tccactagta acggccgcca gtgtgctgga ttaattcgct gtctgcgagg
     1021 gccagctgtt ggggtgagta ctccctctca aagcgggcat gacttctgcg ctagatgtca
     1081 gtttcaaaac gaggaggatt tgatatcacc tggccgcggt gatgcccttt gag
//
