LOCUS       sequence-260377-        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of GFP (as control) tagged with N-terminal
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-GFP_IDG-K
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    88..112
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(132..842,843..845,846..848))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(174..195)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     523..542
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     782..803
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(869..893)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(903..926)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      926..935
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1005..1029)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(1026..1078)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(1056..1076)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctggagcgtc tcaccccaac agctggccct cgcagacagc gaattaattc cagcacactg
       61 gcggccgtta ctagtggatc ttaatcaacc actttgtaca agaaagctgg gtcggcgcgc
      121 ccacccttgg acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
      181 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg
      241 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      301 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcgcc
      361 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
      421 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
      481 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
      541 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
      601 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg
      661 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
      721 agcttgccgt aggtggcatc gccctcgccc tcgccagaca cgctgaactt gtggccgttt
      781 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
      841 ctcaccatgg tgaagggggc ggccgcggag cctgcttttt tgtacaaact tgttgatccg
      901 cccttgtcgt cgtcgtcctt gtagtccatg gtggcctcga gctgaccggt atggccgccc
      961 gtgtcttcta tggaggtcaa aacagcgtga tggcgtctcc aggcgatctg acggttcact
     1021 aaacgagctc tgcttatata gacctcccca ccgtacacgc ctaccgccca tttgcgtcaa
     1081 tgngncgaa
//
