LOCUS       sequence-260378-       12674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDK13 (human) tagged with N-terminal 
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12674)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 12674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-CDK13_IDG-K
FEATURES             Location/Qualifiers
     source          1..12674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             5..638
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    685..810
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1307..1540
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1725..1769
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2830..2839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2839..2862
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2872..2896
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5453..5464
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7463..7487)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          7561..7790
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     7773..7792
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(7848..8422,8423..8425,8426..8434)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8015..8032)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8242..8261
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8454..9053
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(8454..8473)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8950..8970
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9068..9656
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9121..9141)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9539..9550)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9731..9964
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10083..10101)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10169..10273
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10274..10342,10343..11134)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10492..10511)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11305..11893
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11794..11813
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12047..12064
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12304..12325
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12340..12345,12346..12363,12364..12370)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12378..12394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12383..12405
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12402..12418
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12402..12418
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12468..12487
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
       61 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
      121 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
      181 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
      241 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
      301 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
      361 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
      421 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
      481 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      541 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      601 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      661 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      721 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      781 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      841 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      901 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      961 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1021 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1081 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1141 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1201 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1261 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1321 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1381 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1441 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1501 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1561 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1621 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1681 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1741 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1801 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     1861 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     1921 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     1981 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgtaccggt
     2821 cagctcgagg ccaccatgga ctacaaggac gacgacgaca agggcggatc aacaagtttg
     2881 tacaaaaaag cagaagggcc gtcaaggccc accatgccga gcagctcgga cacggcgctg
     2941 gggggaggcg ggggcctgag ctgggcggag aagaagttgg aggaacgccg caagcggagg
     3001 cgattcctgt cccctcagca gccgccgctg ctgttgccgc tcctgcagcc gcagctcctg
     3061 caaccgccgc cgcccccgcc gcctctgctc ttcctggctg ctcccggcac ggccgccgcc
     3121 gcagccgccg ccgccgcggc ctcctcctct tgcttcagcc cgggcccccc tctggaggtc
     3181 aagcggctgg cgagaggcaa gaggcgcgca ggagggcggc agaagcggcg tcgcgggccc
     3241 cgcgccgggc aggaggcgga gaagcgtcgg gtcttctcgc tgccccagcc gcagcaggac
     3301 ggcggtggcg gtgctagtag cggcgggggt gtgaccccgc tggtggaata cgaggatgtg
     3361 agctcccagt ccgagcaggg gctgctgctg gggggggcca gcgcggcaac ggcggcgacg
     3421 gctgccgggg gaacgggggg cagcggcggg agtccggcct cctcctccgg cacccagcgg
     3481 cgcggggagg ggtcggagcg caggccccgc cgggaccgcc gcagcagcag tggccgcagc
     3541 aaggagcgcc accgcgagca ccggcggcgg gatgggcagc gcggtggcag cgaggcctcc
     3601 aagtcccgca gccgccacag ccacagcggc gaggaacggg ccgaggtcgc caagagcggc
     3661 agcagcagca gcagcggcgg ccgccggaaa agcgcttcgg ccacatccag cagcagtagc
     3721 agccgcaagg accgggactc gaaggcccac cgcagccgga ctaagtcgtc caaggagccg
     3781 ccttcggcct acaaggaacc gcccaaggcc taccgggagg acaagaccga gcctaaggcc
     3841 tacaggcggc ggcggtccct cagcccactg ggaggccggg acgacagccc ggtgtcccac
     3901 agggcctctc agagcctgag gagccgcaag tcccccagcc cggcaggagg tggcagcagc
     3961 ccctattctc ggcggctgcc gcgctccccg agcccctaca gtcgccgccg ctcccccagc
     4021 tacagccgcc acagctccta cgagcggggc ggcgacgtgt cccctagtcc ctacagcagc
     4081 agcagctggc gccgctctcg cagtccctac agccctgtgc tcagacggtc tggaaaatcc
     4141 cgaagcagaa gcccgtattc atctaggcat tcaagatctc gtagcaggca cagattgtct
     4201 agatccagaa gtcgtcattc tagtatttct cctagcacac taactctgaa gagtagcctg
     4261 gcagctgaat tgaacaagaa taaaaaagca cgagcagcag aggcagcaag agccgcagaa
     4321 gcagcgaaag ctgcagaagc aactaaggct gctgaggctg ctgccaaggc tgcaaaagct
     4381 tcaaacactt ctacacctac caaggggaac acggaaacta gtgccagtgc atcacaaaca
     4441 aaccatgtga aggatgtgaa gaaaattaaa attgaacatg caccttctcc ctcaagtggt
     4501 ggaactttaa aaaatgacaa agcaaaaaca aagccacctc ttcaggtaac gaaggtggaa
     4561 aataatttga ttgtagataa agccaccaag aaagcagtca tagttggaaa ggagagtaaa
     4621 tctgctgcta caaaggagga atcagtatct cttaaagaga aaaccaaacc acttacacca
     4681 agcataggag ccaaggagaa ggagcaacat gtagctttag tcacctctac attaccaccg
     4741 ttacctttgc ctcccatgct gcctgaagat aaagaagctg atagcttacg aggaaatatt
     4801 tcagtaaaag cagttaaaaa agaagtagaa aagaaactcc gatgtcttct tgctgattta
     4861 ccgctgcccc ctgagctacc aggaggagat gatctttcaa agagtccaga ggaaaagaaa
     4921 acagcaacac agttacatag taaaaggagg cctaaaatat gtgggcctcg ctatggtgaa
     4981 accaaagaaa aagatattga ctggggaaaa cgctgcgtgg ataaatttga tatcatcgga
     5041 attattggag aaggtactta cggacaagtt tacaaagcca gggataaaga cactggagaa
     5101 atggtagcct taaaaaaagt acgtctggat aatgaaaagg aaggctttcc aattacagca
     5161 attcgagaaa ttaaaattct ccggcagctt acccatcaga gtattatcaa tatgaaggaa
     5221 atagtgactg ataaagaaga tgctttggat ttcaagaagg acaaaggtgc attttatctg
     5281 gtgtttgaat atatggacca tgatctgatg ggactactgg aatcaggctt ggttcatttt
     5341 aatgaaaatc acataaagtc atttatgaga cagctcatgg agggtctgga ttattgtcat
     5401 aagaagaact ttttgcatag agatattaaa tgttccaata tccttctaaa taatagaggg
     5461 cagataaaac ttgcagactt tggacttgct cgattgtata gctcagaaga aagtcggccg
     5521 tatactaaca aggtaattac tttatggtac cgtccacctg aactgctact gggagaagaa
     5581 cgatacacac cagccattga tgtatggagc tgtggctgta tccttggcga actcttcact
     5641 aaaaaaccta tatttcaagc aaatcaggaa cttgcacaac tagaattaat aagccgaata
     5701 tgtgggagtc catgtcctgc agtgtggcct gatgtaatca aactaccata tttcaacacc
     5761 atgaaaccaa agaagcaata tcgtcgaaag ttaagagaag aatttgtttt tattcctgca
     5821 gctgcgctag acttatttga ttacatgctt gccttggatc ctagtaagcg ctgcactgct
     5881 gaacaggctc ttcagtgcga gttcctccga gatgtggaac cctcaaaaat gcctccacca
     5941 gatctccctt tatggcaaga ttgtcatgag ttatggagta aaaagcgaag aagacagaag
     6001 cagatgggca tgactgatga tgtttccaca attaaagccc ccaggaagga cttgtctctg
     6061 ggcttggatg acagcagaac caacacaccc cagggtgtgc tgccatcttc acagctgaaa
     6121 tctcagggca gctcaaatgt ggcacctgta aaaacaggcc ctggacagca cttaaaccac
     6181 agtgaattgg caattctact aaacctacta caatctaaaa caagtgttaa tatggctgat
     6241 tttgtccaag tgttgaacat taaggtaaac tctgagactc aacagcagct aaataaaata
     6301 aaccttcctg ctggaatttt ggcaacaggt gaaaaacaga cagatccatc aacaccacaa
     6361 caggagtctt cgaaaccgtt gggaggaatt cagccttctt ctcagaccat ccagcctaaa
     6421 gtggagactg atgctgccca ggcggctgtg cagagtgcat ttgcagttct gttgactcag
     6481 ttaataaagg ctcagcagtc aaagcagaaa gatgtgctac tagaagagag ggaaaatgga
     6541 tcgggacatg aagcgtcatt acaactcagg ccacctccag aacctagcac tccggtgtcg
     6601 ggacaagatg acctcatcca gcatcaagat atgaggatct tggagctaac gccagaacca
     6661 gaccggcctc gaattctgcc tcctgaccaa cgacctcccg agcctcctga accaccacca
     6721 gtcactgagg aagatctaga ttatcggaca gaaaaccagc atgtacccac caccagttct
     6781 tcattaactg accctcatgc cggagtgaag gcagccctgt tacagctgct tgctcagcat
     6841 cagccccagg atgaccccaa aagagaaggt gggattgatt atcaagcagg agacacttac
     6901 gtgtccactt cagactacaa ggacaacttt ggatcctctt ctttctcttc tgctccttat
     6961 gttagcaatg atggtctagg aagcagttct gctccaccac tagaacgacg tagtttcatt
     7021 ggaaattcag atattcagtc tttggataac tacagtactg cttcatctca ttctggtggt
     7081 ccacctcagc cttctgcctt ttctgagtca tttcccagtt cagtagctgg atatggagac
     7141 atttacctca atgctggtcc catgttgttt agtggagaca aggaccatag atttgaatat
     7201 agccatggtc ctattgcagt cctggcaaac agcagtgacc cttccacggg gccagagagt
     7261 actcatcctt tgccagcaaa gatgcacaac tataactatg gtggtaactt acaggaaaat
     7321 ccgagtggcc ccagcctcat gcatggacag acctggactt ctcctgccca aggacctgga
     7381 tattcacaag gatacagggg acatattagc acatcaactg gcagaggcag aggcagaggg
     7441 ttaccatact agggcctcat gggcccagct ttcttgtaca aagtggttga ttaagatcca
     7501 ctagtaacgg ccgccagtgt gctggaatta attcgctgtc tgcgagggcc agctgttggg
     7561 gtgagtactc cctctcaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa
     7621 acgaggagga tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcgtcca
     7681 tctggtcaga aaagacaatc tttttgttgt caagcttgag gtgtggcagg cttgagatct
     7741 ggccatacac ttgagtgaca atgacatcca ctttgccttt ctctccacag gtgtccactc
     7801 ccaggtccaa ctgcaggtcg agcatgcatc tagggcggcc aattccgccc ctctccctcc
     7861 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     7921 atgtgatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     7981 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     8041 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     8101 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     8161 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     8221 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     8281 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     8341 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     8401 ctttgaaaaa cacgatgata agcttgccac aacccacaag gagacgacct tccatgaccg
     8461 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc
     8521 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca
     8581 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     8641 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     8701 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     8761 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     8821 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     8881 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     8941 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     9001 agtgcccgaa ggaccgcgcg acctggtgca tgacccgcaa gcccggtgcc tgatatcgat
     9061 agatcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     9121 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     9181 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     9241 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     9301 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     9361 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     9421 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     9481 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     9541 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     9601 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga
     9661 gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     9721 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     9781 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     9841 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     9901 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     9961 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
    10021 tcagagagtg agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa
    10081 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
    10141 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
    10201 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
    10261 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
    10321 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
    10381 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
    10441 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
    10501 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
    10561 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
    10621 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
    10681 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
    10741 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
    10801 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
    10861 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
    10921 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    10981 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    11041 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    11101 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    11161 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    11221 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    11281 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    11341 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    11401 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    11461 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    11521 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    11581 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    11641 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    11701 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    11761 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    11821 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    11881 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    11941 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    12001 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    12061 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    12121 ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
    12181 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
    12241 aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
    12301 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
    12361 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat
    12421 gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    12481 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
    12541 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
    12601 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
    12661 tactacaaac ttag
//
