LOCUS       sequence-260381-        9605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of PKMYT1 (human) tagged with N-terminal 
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9605)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 9605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-PKMYT1_IDG-K
FEATURES             Location/Qualifiers
     source          1..9605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             5..638
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    685..810
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1307..1540
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1725..1769
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2830..2839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2839..2862
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2872..2896
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4394..4418)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          4492..4721
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4704..4723
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(4779..5353,5354..5356,5357..5365)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4946..4963)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5173..5192
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5385..5984
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(5385..5404)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5881..5901
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5999..6587
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6052..6072)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6470..6481)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6662..6895
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7014..7032)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7100..7204
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7205..7273,7274..8065)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7423..7442)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8236..8824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8725..8744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8978..8995
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9235..9256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9271..9276,9277..9294,9295..9301)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9309..9325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9314..9336
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9333..9349
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9333..9349
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9399..9418
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
       61 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
      121 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
      181 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
      241 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
      301 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
      361 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
      421 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
      481 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      541 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      601 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      661 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      721 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      781 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      841 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      901 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      961 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1021 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1081 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1141 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1201 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1261 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1321 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1381 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1441 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1501 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1561 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1621 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1681 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1741 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1801 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     1861 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     1921 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     1981 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgtaccggt
     2821 cagctcgagg ccaccatgga ctacaaggac gacgacgaca agggcggatc aacaagtttg
     2881 tacaaaaaag ttggcaccat gctagaacgg cctcctgcac tggccatgcc catgcccacg
     2941 gagggcaccc cgccacctct gagtggcacc cccatcccag tcccagccta cttccgccac
     3001 gcagaacctg gattctccct caagaggccc agggggctca gccggagcct cccacctccg
     3061 ccccctgcca agggcagcat tcccatcagc cgcctcttcc ctcctcggac cccaggctgg
     3121 caccagctgc agccccggcg ggtgtcattc cggggcgagg cctcagagac tctgcagagc
     3181 cctgggtatg acccaagccg gccagagtcc ttcttccagc agagcttcca gaggctcagc
     3241 cgcctgggcc atggctccta cggagaggtc ttcaaggtgc gctccaagga ggacggccgg
     3301 ctctatgcgg taaagcgttc catgtcacca ttccggggcc ccaaggaccg ggcccgcaag
     3361 ttggccgagg tgggcagcca cgagaaggtg gggcagcacc catgctgcgt gcggctggag
     3421 caggcctggg aggagggcgg catcctgtac ctgcagacgg agctgtgcgg gcccagcctg
     3481 cagcaacact gtgaggcctg gggtgccagc ctgcctgagg cccaggtctg gggctacctg
     3541 cgggacacgc tgcttgccct ggcccatctg cacagccagg gcctggtgca ccttgatgtc
     3601 aagcctgcca acatcttcct ggggccccgg ggccgctgca agctgggtga cttcggactg
     3661 ctggtggagc tgggtacagc aggagctggt gaggtccagg agggagaccc ccgctacatg
     3721 gcccccgagc tgctgcaggg ctcctatggg acagcagcgg atgtgttcag tctgggcctc
     3781 accatcctgg aagtggcatg caacatggag ctgccccacg gtggggaggg ctggcagcag
     3841 ctgcgccagg gctacctgcc ccctgagttc actgccggtc tgtcttccga gctgcgttct
     3901 gtccttgtca tgatgctgga gccagacccc aagctgcggg ccacggccga ggccctgctg
     3961 gcactgcctg tgttgaggca gccgcgggcc tggggtgtgc tgtggtgcat ggcagcggag
     4021 gccctgagcc gagggtgggc cctgtggcag gccctgcttg ccctgctctg ctggctctgg
     4081 catgggctgg ctcaccctgc cagctggcta cagcccctgg gcccgccagc caccccgcct
     4141 ggctcaccac cctgcagttt gctcctggac agcagcctct ccagcaactg ggatgacgac
     4201 agcctagggc cttcactctc ccctgaggct gtcctggccc ggactgtggg gagcacctcc
     4261 accccccgga gcaggtgcac acccagggat gccctggacc taagtgacat caactcagag
     4321 cctcctcggg gctccttccc ctcctttgag cctcggaacc tcctcagcct gtttgaggac
     4381 accctagacc caaccccaac tttcttgtac aaagtggttg attaagatcc actagtaacg
     4441 gccgccagtg tgctggaatt aattcgctgt ctgcgagggc cagctgttgg ggtgagtact
     4501 ccctctcaaa agcgggcatg acttctgcgc taagattgtc agtttccaaa aacgaggagg
     4561 atttgatatt cacctggccc gcggtgatgc ctttgagggt ggccgcgtcc atctggtcag
     4621 aaaagacaat ctttttgttg tcaagcttga ggtgtggcag gcttgagatc tggccataca
     4681 cttgagtgac aatgacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca
     4741 actgcaggtc gagcatgcat ctagggcggc caattccgcc cctctccctc ccccccccct
     4801 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgtgattt
     4861 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     4921 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     4981 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     5041 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     5101 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     5161 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     5221 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     5281 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     5341 acacgatgat aagcttgcca caacccacaa ggagacgacc ttccatgacc gagtacaagc
     5401 ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg
     5461 cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg
     5521 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     5581 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     5641 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     5701 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     5761 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     5821 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     5881 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gagtgcccga
     5941 aggaccgcgc gacctggtgc atgacccgca agcccggtgc ctgatatcga tagatcctaa
     6001 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6061 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6121 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6181 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6241 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6301 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6361 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6421 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6481 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6541 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta
     6601 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6661 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     6721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     6901 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     6961 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
     7021 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     7081 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7141 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7201 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7261 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7321 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7381 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7441 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7501 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7561 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7621 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7681 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7741 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7801 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7861 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7921 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7981 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8041 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8101 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8161 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8221 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8281 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8341 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8401 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8461 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8521 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8581 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8641 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8701 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8761 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8821 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8881 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     8941 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9001 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9061 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     9121 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     9181 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     9241 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     9301 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     9361 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     9421 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     9481 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     9541 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     9601 cttag
//
