LOCUS       sequence-260382-        1164 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of PKMYT1 (human) tagged with N-terminal 
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1164)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-PKMYT1_IDG-K
FEATURES             Location/Qualifiers
     source          1..1164
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      118..127
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             127..150
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    160..184
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcncattagg tggcaaatag cagagctcgt ttagtgaccg tcagatcgcc tggagacgcc
       61 atccacgctg ttttgacctc catagaagac accggcggcc ataccggtca gctcgaggcc
      121 accatggact acaaggacga cgacgacaag ggcggatcaa caagtttgta caaaaaagtt
      181 ggcaccatgc tagaacggcc tcctgcactg gccatgccca tgcccacgga gggcaccccg
      241 ccacctctga gtggcacccc catcccagtc ccagcctact tccgccacgc agaacctgga
      301 ttctccctca agaggcccag ggggctcagc cggagcctcc cacctccgcc ccctgccaag
      361 ggcagcattc ccatcagccg cctcttccct cctcggaccc caggctggca ccagctgcag
      421 ccccggcggg tgtcattccg gggcgaggcc tcagagactc tgcagagccc tgggtatgac
      481 ccaagccggc cagagtcctt cttccagcag agcttccaga ggctcagccg cctgggccat
      541 ggctcctacg gagaggtctt caaggtgcgc tccaaggagg acggccggct ctatgcggta
      601 aagcgttcca tgtcaccatt ccggggcccc aaggaccggg cccgcaagtt ggccgaggtg
      661 ggcagccacg agaaggtggg gcagcaccca tgctgcgtgc ggctggagca ggcctgggag
      721 gagggcggca tcctgtacct gcagacggag ctgtgcgggc ccagcctgca gcaacactgt
      781 gaggcctggg gtgccagcct gcctgaggcc catgtctggg gctacctgcg ggacacgctg
      841 cttgccctgg cccatctgca cagccagggc ctggtgcacc ttgatgtcaa gcctgccaac
      901 atcttcctgg ggccccgggg ccgctgcaag ctgggtgact tcggactgct ggtggagctg
      961 ggtacagcag agctggtgag gtccaggagg agaccccgct acatggcccc gagctgctgc
     1021 aggctcctat gggacagcag cggatgtgtt cagtctgggc ctcaccatct gnantgcatg
     1081 cacatgagct gccccacgng ggggaggcct gcagcagctg cccagggcct actgcccctg
     1141 agttcactgc ggtctgttct ttcc
//
