LOCUS       sequence-260384-       10421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of TLK2 (human) tagged with N-terminal 
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10421)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-TLK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..10421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             5..638
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    685..810
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1307..1540
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1725..1769
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2830..2839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2839..2862
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2872..2896
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3474..3485)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(5210..5234)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5308..5537
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5520..5539
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(5595..6169,6170..6172,6173..6181)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5762..5779)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5989..6008
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6201..6800
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(6201..6220)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6697..6717
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6815..7403
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6868..6888)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7286..7297)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7478..7711
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7830..7848)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7916..8020
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8021..8089,8090..8881)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8239..8258)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9052..9640
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9541..9560
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9794..9811
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10051..10072
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10087..10092,10093..10110,10111..10117)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10125..10141
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10130..10152
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10149..10165
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10149..10165
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10215..10234
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
       61 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
      121 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
      181 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
      241 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
      301 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
      361 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
      421 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
      481 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      541 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      601 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      661 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      721 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      781 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      841 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      901 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      961 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1021 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1081 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1141 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1201 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1261 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1321 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1381 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1441 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1501 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1561 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1621 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1681 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1741 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1801 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     1861 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     1921 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     1981 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgtaccggt
     2821 cagctcgagg ccaccatgga ctacaaggac gacgacgaca agggcggatc aacaagtttg
     2881 tacaaaaaag ttggcatgga agaattgcat agcctggacc cacgacggca ggaattattg
     2941 gaggccaggt ttactggagt aggtgttagt aagggaccac ttaatagtga gtcttccaac
     3001 cagagcttgt gcagcgtcgg atccttgagt gataaagaag tagagactcc cgagaaaaag
     3061 cagaatgacc agcgaaatcg gaaaagaaaa gctgaaccat atgaaactag ccaagggaaa
     3121 ggcactccta ggggacataa aattagtgat tactttgagt ttgctggggg aagcgcgcca
     3181 ggaaccagcc ctggcagaag tgttccacca gttgcacgat cctcaccgca acattcctta
     3241 tccaatccct taccgcgacg agtagaacag cccctctatg gtttagatgg cagtgctgca
     3301 aaggaggcaa cggaggagca gtctgctctg ccaaccctca tgtcagtgat gctagcaaaa
     3361 cctcggcttg acacagagca gctggcgcaa aggggagctg gcctctgctt cacttttgtt
     3421 tcagctcagc aaaacagtcc ctcatctacg ggatctggca acacagagca ttcctgcagc
     3481 tcccaaaaac agatctccat ccagcacaga cagacccagt ccgacctcac aatagaaaaa
     3541 atatctgcac tagaaaacag taagaattct gacttagaga agaaggaggg aagaatagat
     3601 gatttattaa gagccaactg tgatttgaga cggcagattg atgaacagca aaagatgcta
     3661 gagaaataca aggaacgatt aaatagatgt gtgacaatga gcaagaaact ccttatagaa
     3721 aagtcaaaac aagagaagat ggcgtgtaga gataagagca tgcaagaccg cttgagactg
     3781 ggccacttta ctactgtccg acacggagcc tcatttactg aacagtggac agatggttat
     3841 gcttttcaga atcttatcaa gcaacaggaa aggataaatt cacagaggga agagatagaa
     3901 agacaacgga aaatgttagc aaagcggaaa cctcctgcca tgggtcaggc ccctcctgca
     3961 accaatgagc agaaacagcg gaaaagcaag accaatggag ctgaaaatga aacgccctct
     4021 tctgggaata cagagctaaa ggatacagcc ccagccttag gagcccacag tttacttagg
     4081 ttaacgttag cagaatacca tgaacaagaa gaaatcttca aactcagatt aggtcatctt
     4141 aaaaaggagg aagcagagat ccaggcagag ctggagagac tagaaagggt tagaaatcta
     4201 catatcaggg aactaaaaag gatacataat gaagataatt cacaatttaa agatcatcca
     4261 acgctaaatg acagatattt gttgttacat cttttgggta gaggaggttt cagtgaagtt
     4321 tacaaggcat ttgatctaac agagcaaaga tacgtagctg tgaaaattca ccagttaaat
     4381 aaaaactgga gagatgagaa aaaggagaat taccacaagc atgcatgtag ggaataccgg
     4441 attcataaag agctggatca tcccagaata gttaagctgt atgattactt ttcactggat
     4501 actgactcgt tttgtacagt attagaatac tgtgagggaa atgatctgga cttctacctg
     4561 aaacagcaca aattaatgtc ggagaaagag gcccggtcca ttatcatgca gattgtgaat
     4621 gctttaaagt acttaaatga aataaaacct cccatcatac actatgacct caaaccaggt
     4681 aatattcttt tagtaaatgg tacagcgtgt ggagagataa aaattacaga ttttggtctt
     4741 tcgaagatca tggatgatga tagctacaat tcagtggatg gcatggagct aacatcacaa
     4801 ggtgctggta cttattggta tttaccacca gagtgttttg tggttgggaa agaaccacca
     4861 aagatctcaa ataaagttga tgtgtggtcg gtgggtgtga tcttctatca gtgtctttat
     4921 ggaaggaagc cttttggcca taaccagtct cagcaagaca tcctacaaga gaatacgatt
     4981 cttaaagcta ctgaagtgca gttcccgcca aagccagtag taacacctga agcaaaggcg
     5041 tttattcgac gatgcttggc ctaccgaaag gaggaccgca ttgatgtcca gcagctggcc
     5101 tgtgatccct acttgttgcc tcacatccga aagtcagtct ctacaagtag ccctgctgga
     5161 gctgctattg catcaacctc tggggcgtcc aataacagtt cttctaattg cccaactttc
     5221 ttgtacaaag tggttgatta agatccacta gtaacggccg ccagtgtgct ggaattaatt
     5281 cgctgtctgc gagggccagc tgttggggtg agtactccct ctcaaaagcg ggcatgactt
     5341 ctgcgctaag attgtcagtt tccaaaaacg aggaggattt gatattcacc tggcccgcgg
     5401 tgatgccttt gagggtggcc gcgtccatct ggtcagaaaa gacaatcttt ttgttgtcaa
     5461 gcttgaggtg tggcaggctt gagatctggc catacacttg agtgacaatg acatccactt
     5521 tgcctttctc tccacaggtg tccactccca ggtccaactg caggtcgagc atgcatctag
     5581 ggcggccaat tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
     5641 gaataaggcc ggtgtgcgtt tgtctatatg tgattttcca ccatattgcc gtcttttggc
     5701 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     5761 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     5821 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     5881 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     5941 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     6001 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     6061 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     6121 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac
     6181 ccacaaggag acgaccttcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     6241 acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     6301 gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     6361 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     6421 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     6481 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     6541 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     6601 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     6661 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     6721 tcggcttcac cgtcaccgcc gacgtcgagt gcccgaagga ccgcgcgacc tggtgcatga
     6781 cccgcaagcc cggtgcctga tatcgataga tcctaatcaa cctctggatt acaaaatttg
     6841 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     6901 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     6961 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7021 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7081 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7141 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7201 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     7261 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7321 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     7381 ctccctttgg gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct
     7441 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     7501 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     7561 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7621 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7681 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     7741 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa
     7801 agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     7861 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     7921 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7981 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     8041 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8101 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8161 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     8221 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     8281 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8341 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8401 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8461 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8521 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     8581 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8641 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8701 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8761 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8821 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8881 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8941 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     9001 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     9061 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     9121 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     9181 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     9241 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     9301 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     9361 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     9421 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     9481 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     9541 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     9601 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     9661 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     9721 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     9781 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     9841 gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt
     9901 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     9961 ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga
    10021 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
    10081 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    10141 atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc
    10201 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    10261 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    10321 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
    10381 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta g
//
