LOCUS       sequence-260385-        1166 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of TLK2 (human) tagged with N-terminal 
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1166)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-TLK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..1166
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             122..145
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    155..179
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(757..768)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 cnntttttgt attagcagag ctcgtttagt gaccgtcaga tcgcctggag acgccatcca
       61 cgctgttttg acctccatag aagacaccgg cggccatacc ggtcagctcg aggccaccat
      121 ggactacaag gacgacgacg acaagggcgg atcaacaagt ttgtacaaaa aagttggcat
      181 ggaagaattg catagcctgg acccacgacg gcaggaatta ttggaggcca ggtttactgg
      241 agtaggtgtt agtaagggac cacttaatag tgagtcttcc aaccagagct tgtgcagcgt
      301 cggatccttg agtgataaag aagtagagac tcccgagaaa aagcagaatg accagcgaaa
      361 tcggaaaaga aaagctgaac catatgaaac tagccaaggg aaaggcactc ctaggggaca
      421 taaaattagt gattactttg agtttgctgg gggaagcgcg ccaggaacca gccctggcag
      481 aagtgttcca ccagttgcac gatcctcacc gcaacattcc ttatccaatc ccttaccgcg
      541 acgagtagaa cagcccctct atggtttaga tggcagtgct gcaaaggagg caacggagga
      601 gcagtctgct ctgccaaccc tcatgtcagt gatgctagca aaacctcggc ttgacacaga
      661 gcagctggcg caaaggggag ctggcctctg cttcactttt gtttcagctc agcaaaacag
      721 tccctcatct acgggatctg gcaacacaga gcattcctgc agctcccaaa aacagatctc
      781 catccagcac agacagaccc agtccgacct cacaatagaa aaaatatctg cactagaaaa
      841 cagtaagaat tctgacttag agaagaggga gggaagaata gatgatttat taagagccaa
      901 ctgtgatttg agacggcaga ttgatgaaca gcaaagatgc tagagaaata caaagaacga
      961 ttaaatagat gtgtgacaat gagcaagaaa ctccttatag aaaagtcaaa acaagagaag
     1021 gatggcgtgt agaagataag agcatgcaag accgcttgaa gactgggcca ctttactact
     1081 gtccgaccgg agctcattta ctgaacagtg gacagaatgg gttatgcttt tcgaatctta
     1141 tcaagcacag ggaaaggaat aaaatc
//
