LOCUS       sequence-260387-       10142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK2 (human) tagged with N-terminal 
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10142)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-BRSK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..10142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             5..638
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    685..810
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1307..1540
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1725..1769
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2830..2839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2839..2862
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2872..2896
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4931..4955)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5029..5258
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5241..5260
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(5316..5890,5891..5893,5894..5902)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5483..5500)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5710..5729
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5922..6521
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(5922..5941)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6418..6438
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6536..7124
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6589..6609)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7007..7018)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7199..7432
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7551..7569)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7637..7741
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7742..7810,7811..8602)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7960..7979)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8773..9361
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9262..9281
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9515..9532
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9772..9793
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9808..9813,9814..9831,9832..9838)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9846..9862
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9851..9873
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9870..9886
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9870..9886
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9936..9955
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
       61 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
      121 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
      181 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
      241 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
      301 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
      361 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
      421 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
      481 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      541 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      601 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      661 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      721 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      781 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      841 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      901 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      961 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1021 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1081 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1141 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1201 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1261 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1321 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1381 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1441 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1501 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1561 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1621 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1681 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1741 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1801 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     1861 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     1921 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     1981 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgtaccggt
     2821 cagctcgagg ccaccatgga ctacaaggac gacgacgaca agggcggatc aacaagtttg
     2881 tacaaaaaag cagaagggcc gtcaaggccc accatgacat cgacggggaa ggacggcggc
     2941 gcgcagcacg cgcagtatgt tgggccctac cggctggaga agacgctggg caaggggcag
     3001 acaggtctgg tgaagctggg ggttcactgc gtcacctgcc agaaggtggc catcaagatc
     3061 gtcaaccgtg agaagctcag cgagtcggtg ctgatgaagg tggagcggga gatcgcgatc
     3121 ctgaagctca ttgagcaccc ccacgtccta aagctgcacg acgtttatga aaacaaaaaa
     3181 tatttgtacc tggtgctaga acacgtgtca ggtggtgagc tcttcgacta cctggtgaag
     3241 aaggggaggc tgacgcctaa ggaggctcgg aagttcttcc ggcagatcat ctctgcgctg
     3301 gacttctgcc acagccactc catatgccac agggatctga aacctgaaaa cctcctgctg
     3361 gacgagaaga acaacatccg catcgcagac tttggcatgg cgtccctgca ggttggcgac
     3421 agcctgttgg agaccagctg tgggtccccc cactacgcct gccccgaggt gatccggggg
     3481 gagaagtatg acggccggaa ggcggacgtg tggagctgcg gcgtcatcct gttcgccttg
     3541 ctggtggggg ctctgccctt cgacgatgac aacttgcgac agctgctgga gaaggtgaag
     3601 cggggcgtgt tccacatgcc gcactttatc ccgcccgact gccagagtct gctacggggc
     3661 atgatcgagg tggacgccgc acgccgcctc acgctagagc acattcagaa acacatatgg
     3721 tatatagggg gcaagaatga gcccgaacca gagcagccca ttcctcgcaa ggtgcagatc
     3781 cgctcgctgc ccagcctgga ggacatcgac cccgacgtgc tggacagcat gcactcactg
     3841 ggctgcttcc gagaccgcaa caagctgctg caggacctgc tgtccgagga ggagaaccag
     3901 gagaagatga tttacttcct cctcctggac cggaaagaaa ggtacccgag ccaggaggat
     3961 gaggacctgc ccccccggaa cgagatagac cctccccgga agcgtgtgga ctccccgatg
     4021 ctgaaccggc acggcaagcg gcggccagaa cgcaaatcca tggaggtgct cagcgtgacg
     4081 gacggcggct ccccggtgcc tgcgcggcgg gccattgaga tggcccagca cggccagagg
     4141 tctcggtcca tcagcggtgc ctcctcaggc ctttccacca gcccactcag cagcccccgg
     4201 gtgacccctc acccctcacc aaggggcagt cccctcccca cccccaaggg gacacctgtc
     4261 cacacgccaa aggagagccc ggctggcacg cccaacccca cgcccccgtc cagccccagc
     4321 gtcggagggg tgccctggag ggcgcggctc aactccatca agaacagctt tctgggctca
     4381 ccccgcttcc accgccggaa actgcaagtt ccgacgccgg aggagatgtc caacctgaca
     4441 ccagagtcgt ccccagagct ggcgaagaag tcctggtttg ggaacttcat cagcctggag
     4501 aaggaggagc agatcttcgt ggtcatcaaa gacaaacctc tgagctccat caaggctgac
     4561 atcgtgcacg ccttcctgtc gattcccagt ctcagccaca gcgtcatctc ccaaacgagc
     4621 ttccgggccg agtacaaggc cacggggggg ccagccgtgt tccagaagcc ggtcaagttc
     4681 caggttgata tcacctacac ggagggtggg gaggcgcaga aggagaacgg catctactcc
     4741 gtcaccttca ccctgctctc aggccccagc cgtcgcttca agagggtggt ggagaccatc
     4801 caggcccagc tgctgagcac acacgacccg cctgcggccc agcacttgtc agacaccact
     4861 aactgtatgg aaatgatgac ggggcggctt tccaaatgtg gaattatccc gaaaagttca
     4921 ggcctcatgg gcccagcttt cttgtacaaa gtggttgatt aagatccact agtaacggcc
     4981 gccagtgtgc tggaattaat tcgctgtctg cgagggccag ctgttggggt gagtactccc
     5041 tctcaaaagc gggcatgact tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt
     5101 tgatattcac ctggcccgcg gtgatgcctt tgagggtggc cgcgtccatc tggtcagaaa
     5161 agacaatctt tttgttgtca agcttgaggt gtggcaggct tgagatctgg ccatacactt
     5221 gagtgacaat gacatccact ttgcctttct ctccacaggt gtccactccc aggtccaact
     5281 gcaggtcgag catgcatcta gggcggccaa ttccgcccct ctccctcccc cccccctaac
     5341 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gtgattttcc
     5401 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     5461 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     5521 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     5581 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     5641 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     5701 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     5761 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     5821 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     5881 cgatgataag cttgccacaa cccacaagga gacgaccttc catgaccgag tacaagccca
     5941 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
     6001 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
     6061 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     6121 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     6181 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     6241 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     6301 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     6361 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     6421 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag tgcccgaagg
     6481 accgcgcgac ctggtgcatg acccgcaagc ccggtgcctg atatcgatag atcctaatca
     6541 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     6601 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     6661 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     6721 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     6781 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6841 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6901 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     6961 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     7021 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     7081 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga
     7141 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7201 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     7261 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7321 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7381 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     7441 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     7501 aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat
     7561 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     7621 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7681 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7741 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7801 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7861 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7921 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7981 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     8041 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     8101 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     8161 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     8221 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     8281 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     8341 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     8401 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8461 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     8521 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8581 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8641 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8701 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8761 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8821 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8881 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     8941 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9001 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     9061 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     9121 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     9181 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     9241 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     9301 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     9361 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9421 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     9481 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     9541 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa
     9601 gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     9661 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc
     9721 gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     9781 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     9841 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt
     9901 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9961 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
    10021 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
    10081 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
    10141 ag
//
