LOCUS       sequence-260388-        1223 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK2 (human) tagged with N-terminal 
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1223)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-BRSK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..1223
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      114..123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             123..146
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    156..180
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gantgggggg caatagcaga gctcgtttag tgaccgtcag atcgcctgga gacgccatcc
       61 acgctgtttt gacctccata gaagacaccg gcggccatac cggtcagctc gaggccacca
      121 tggactacaa ggacgacgac gacaagggcg gatcaacaag tttgtacaaa aaagcagaag
      181 ggccgtcaag gcccaccatg acatcgacgg ggaaggacgg cggcgcgcag cacgcgcagt
      241 atgttgggcc ctaccggctg gagaagacgc tgggcaaggg gcagacaggt ctggtgaagc
      301 tgggggttca ctgcgtcacc tgccagaagg tggccatcaa gatcgtcaac cgtgagaagc
      361 tcagcgagtc ggtgctgatg aaggtggagc gggagatcgc gatcctgaag ctcattgagc
      421 acccccacgt cctaaagctg cacgacgttt atgaaaacaa aaaatatttg tacctggtgc
      481 tagaacacgt gtcaggtggt gagctcttcg actacctggt gaagaagggg aggctgacgc
      541 ctaaggaggc tcggaagttc ttccggcaga tcatctctgc gctggacttc tgccacagcc
      601 actccatatg ccacagggat ctgaaacctg aaaacctcct gctggacgag aagaacaaca
      661 tccgcatcgc agactttggc atggcgtccc tgcaggttgg cgacagcctg ttggagacca
      721 gctgtgggtc cccccactac gcctgccccg aggtgatccg gggggagaag tatgacggcc
      781 ggaaggcgga cgtgtggagc tgcggcgtca tcctgttcgc cttgctggtg ggggctctgc
      841 ccttcgacga tgacaacttg cgacagctgc tggagaaggt gaagcggggc gtgttccaca
      901 tgccgcactt tatcccgccc gactgccaga gtctgctacg gggcatgatc gagtggacgc
      961 cgcacgccgc ctcacgctag agcacattca gaaacacata tggtatatag gggcaagaat
     1021 gagcccgaac cagagcagcc cattcctcgc aagtgcagat ccgctcgctg ccagcctgag
     1081 acatcgaccc cgacgtgctg gacagcatgc actcactggg ctgctcgaga ccgcacagct
     1141 gctgcagact gctgtcgaga gaaacagnna agatgantnc ctcntcctcc tgaccgaaag
     1201 aagnaccgga ccaggaggat gag
//
