LOCUS       sequence-260390-       10730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of AAK1 (human) tagged with N-terminal 
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10730)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-AAK1_IDG-K
FEATURES             Location/Qualifiers
     source          1..10730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             5..638
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    685..810
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1307..1540
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1725..1769
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2830..2839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2839..2862
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2872..2896
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5519..5543)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          5617..5846
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5829..5848
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(5904..6478,6479..6481,6482..6490)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6071..6088)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6298..6317
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6510..7109
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(6510..6529)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7006..7026
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7124..7712
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7177..7197)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7595..7606)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7787..8020
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8139..8157)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8225..8329
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8330..8398,8399..9190)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8548..8567)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9361..9949
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9850..9869
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10103..10120
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10360..10381
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10396..10401,10402..10419,10420..10426)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10434..10450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10439..10461
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10458..10474
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10458..10474
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10524..10543
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
       61 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
      121 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
      181 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
      241 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
      301 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
      361 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
      421 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
      481 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      541 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      601 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      661 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      721 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      781 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      841 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      901 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      961 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1021 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1081 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1141 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1201 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1261 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1321 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1381 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1441 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1501 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1561 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1621 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1681 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1741 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1801 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     1861 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     1921 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     1981 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgtaccggt
     2821 cagctcgagg ccaccatgga ctacaaggac gacgacgaca agggcggatc aacaagtttg
     2881 tacaaaaaag caggctccga attcgccctt atgaagaagt ttttcgactc ccggcgagag
     2941 cagggcggct ctggcctggg ctccggctcc agcggaggag ggggcagcac ctcgggcctg
     3001 ggcagtggct acatcggaag agtcttcggc atcgggcgac agcaggtcac agtggacgag
     3061 gtgttggcgg aaggtggatt tgctattgta tttctggtga ggacaagcaa tgggatgaaa
     3121 tgtgccttga aacgcatgtt tgtcaacaat gagcatgatc tccaggtgtg caagagagaa
     3181 atccagataa tgagggatct ttcagggcac aagaatattg tgggttacat tgattctagt
     3241 atcaacaacg tgagtagcgg tgatgtatgg gaagtgctca ttctgatgga cttttgtaga
     3301 ggtggccagg tggtaaacct gatgaaccag cgcctgcaaa caggctttac agagaatgaa
     3361 gtgctccaga tattttgtga tacctgtgaa gctgttgccc gcctgcatca gtgcaaaact
     3421 cctattatcc accgggacct gaaggttgaa aacatcctct tgcatgaccg aggccactat
     3481 gtcctgtgtg actttggaag cgccaccaac aaattccaga atccacaaac tgagggagtc
     3541 aatgcagtag aagatgagat taagaaatac acaacgctgt cctatcgagc accagaaatg
     3601 gtcaacctgt acagtggcaa aatcatcact acgaaggcag acatttgggc tcttggatgt
     3661 ttgttgtata aattatgcta cttcactttg ccatttgggg aaagtcaggt ggcaatttgt
     3721 gatggaaact tcacaattcc tgataattct cgatattctc aagacatgca ctgcctaatt
     3781 aggtatatgt tggaaccaga ccctgacaaa aggccggata tttaccaggt gtcctacttc
     3841 tcatttaagc tactcaagaa agagtgccca attccaaatg tacagaactc tcccattcct
     3901 gcaaagcttc ctgaaccagt gaaagccagt gaggcagctg caaaaaagac ccagccaaag
     3961 gccagactga cagatcccat tcccaccaca gagacttcaa ttgcaccccg ccagaggcct
     4021 aaagctgggc agactcagcc gaacccagga atccttccca tccagccagc gctgacaccc
     4081 cggaagaggg ccactgttca gcccccacct caggctgcag gatccagcaa tcagcctggc
     4141 cttttagcca gtgttcccca accaaaaccc caagccccac ccagccagcc tctgccgcaa
     4201 actcaggcca agcagccaca ggctcctccc actccacagc agacgccttc tactcaggcc
     4261 cagggtctgc ccgctcaggc ccaggccaca ccccagcacc agcagcaact cttcctcaag
     4321 cagcaacagc agcagcaaca gccaccgcca gcacagcagc agccggcagg cacgttttac
     4381 cagcagcagc aggcccagac tcagcagttt caggcagtac atccagcaac ccagaaacca
     4441 gcaattgctc agttccctgt ggtgtcccaa ggaggctctc aacagcagct aatgcagaat
     4501 ttctaccagc agcagcagca gcagcaacaa caacagcaac agcaacagct ggccacagcc
     4561 ctgcatcaac aacagctgat gactcagcag gctgccttgc agcaaaagcc cactatggca
     4621 gcaggacagc agccccagcc acagccagct gcagccccac agccagcccc tgcccaggag
     4681 ccagcgattc aagccccagt aagacaacag ccaaaggttc agacaacccc acctcctgcc
     4741 gtccaggggc agaaagttgg atctctcact ccaccctcat cccccaaaac ccaacgtgct
     4801 gggcacaggc gtattctcag tgacgtaacc cacagtgcag tctttggggt ccctgccagc
     4861 aaatcaaccc agctgctcca ggcagctgca gctgaggcca gtctcaataa gtccaagtct
     4921 gcaaccacca ctccatcagg ctctcctcgg acctctcaac aaaacgttta taatccttca
     4981 gaagggtcta cgtggaatcc ctttgatgac gataatttct ccaaactcac agctgaagaa
     5041 ctgctaaaca aggactttgc caagcttggg gaaggcaaac atcccgagaa gcttggaggc
     5101 tcagctgaga gtttgatccc aggctttcaa tcaacccaag gtgatgcttt tgctacgacc
     5161 tcattttctg ctggaactgc tgaaaaaagg aagggtgggc agactgtgga ctctggcctc
     5221 ccgcttctaa gcgtgtctga tcctttcatt cctcttcaag tacctgatgc accagaaaaa
     5281 ctaattgagg gactcaagtc tcctgacact tctcttctgc tccctgacct cttgcctatg
     5341 acagatcctt ttggtagcac ttctgatgct gtaattggta aagtcatcat ctctgtttct
     5401 tcagtcatgc atgatatgtg tgcctgtttc aagaatgaca agtacctagt taaccaatcc
     5461 ctggggaata gccctgccac cccagaagcc aaggctattg aattcaaggg cgaattcgac
     5521 ccagctttct tgtacaaagt ggttgattaa gatccactag taacggccgc cagtgtgctg
     5581 gaattaattc gctgtctgcg agggccagct gttggggtga gtactccctc tcaaaagcgg
     5641 gcatgacttc tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct
     5701 ggcccgcggt gatgcctttg agggtggccg cgtccatctg gtcagaaaag acaatctttt
     5761 tgttgtcaag cttgaggtgt ggcaggcttg agatctggcc atacacttga gtgacaatga
     5821 catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc aggtcgagca
     5881 tgcatctagg gcggccaatt ccgcccctct ccctcccccc cccctaacgt tactggccga
     5941 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt gattttccac catattgccg
     6001 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     6061 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     6121 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     6181 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     6241 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     6301 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     6361 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     6421 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct
     6481 tgccacaacc cacaaggaga cgaccttcca tgaccgagta caagcccacg gtgcgcctcg
     6541 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc
     6601 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag
     6661 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     6721 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     6781 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     6841 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     6901 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     6961 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     7021 acgagcggct cggcttcacc gtcaccgccg acgtcgagtg cccgaaggac cgcgcgacct
     7081 ggtgcatgac ccgcaagccc ggtgcctgat atcgatagat cctaatcaac ctctggatta
     7141 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     7201 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     7261 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     7321 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     7381 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     7441 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     7501 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     7561 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     7621 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     7681 gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac
     7741 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     7801 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     7861 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7921 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7981 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     8041 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta
     8101 attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt
     8161 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     8221 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     8281 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     8341 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     8401 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     8461 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8521 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     8581 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8641 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8701 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8761 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8821 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8881 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8941 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     9001 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     9061 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     9121 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     9181 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     9241 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     9301 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     9361 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9421 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9481 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     9541 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9601 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9661 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9721 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9781 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9841 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9901 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9961 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    10021 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    10081 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
    10141 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
    10201 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
    10261 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
    10321 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
    10381 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
    10441 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
    10501 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10561 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
    10621 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
    10681 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
//
