LOCUS       sequence-260391-        1157 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of AAK1 (human) tagged with N-terminal 
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1157)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-AAK1_IDG-K
FEATURES             Location/Qualifiers
     source          1..1157
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      117..126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             126..149
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    159..183
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 anncaaggac ccacgctagc agagctcgtt tagtgaccgt cagatcgcct ggagacgcca
       61 tccacgctgt tttgacctcc atagaagaca ccggcggcca taccggtcag ctcgaggcca
      121 ccatggacta caaggacgac gacgacaagg gcggatcaac aagtttgtac aaaaaagcag
      181 gctccgaatt cgcccttatg aagaagtttt tcgactcccg gcgagagcag ggcggctctg
      241 gcctgggctc cggctccagc ggaggagggg gcagcacctc gggcctgggc agtggctaca
      301 tcggaagagt cttcggcatc gggcgacagc aggtcacagt ggacgaggtg ttggcggaag
      361 gtggatttgc tattgtattt ctggtgagga caagcaatgg gatgaaatgt gccttgaaac
      421 gcatgtttgt caacaatgag catgatctcc aggtgtgcaa gagagaaatc cagataatga
      481 gggatctttc agggcacaag aatattgtgg gttacattga ttctagtatc aacaacgtga
      541 gtagcggtga tgtatgggaa gtgctcattc tgatggactt ttgtagaggt ggccaggtgg
      601 taaacctgat gaaccagcgc ctgcaaacag gctttacaga gaatgaagtg ctccagatat
      661 tttgtgatac ctgtgaagct gttgcccgcc tgcatcagtg caaaactcct attatccacc
      721 gggacctgaa ggttgaaaac atcctcttgc atgaccgagg ccactatgtc ctgtgtgact
      781 ttggaagcgc caccaacaaa ttccagaatc cacaaactga gggagtcaat gcagtagaag
      841 atgagattaa gaaatacaca acgctgtcct atcgagcacc agaaatggtc aacctgtaca
      901 gtggcaaaat catcactacg aaagcagaca tttgggctct tggatgttgt tgtataaatt
      961 atgctacttc actttgccat ttggggaaag tcagtggcaa tttgtgatgg aaacttcaca
     1021 attcctgaat aattctcgat atctcagaca tgcactgcct aattagggta taatgttgac
     1081 cagaccctga caaacgccga tatttaccag gtgtctactc ctcatttaag ctactcaaga
     1141 aaagaagtgc ccaattt
//
