LOCUS       sequence-260393-       12602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDK12 (human) tagged with N-terminal 
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12602)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 12602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-CDK12_IDG-K
FEATURES             Location/Qualifiers
     source          1..12602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             5..638
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    685..810
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1307..1540
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1725..1769
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2830..2839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2839..2862
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2872..2896
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7391..7415)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          7489..7718
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     7701..7720
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(7776..8350,8351..8353,8354..8362)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7943..7960)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8170..8189
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8382..8981
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(8382..8401)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8878..8898
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8996..9584
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9049..9069)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9467..9478)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9659..9892
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10011..10029)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10097..10201
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10202..10270,10271..11062)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10420..10439)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11233..11821
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11722..11741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11975..11992
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12232..12253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12268..12273,12274..12291,12292..12298)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12306..12322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12311..12333
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12330..12346
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12330..12346
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12396..12415
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
       61 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
      121 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
      181 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
      241 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
      301 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
      361 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
      421 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
      481 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      541 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      601 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      661 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      721 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      781 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      841 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      901 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      961 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1021 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1081 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1141 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1201 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1261 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1321 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1381 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1441 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1501 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1561 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1621 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1681 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1741 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1801 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     1861 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     1921 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     1981 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgtaccggt
     2821 cagctcgagg ccaccatgga ctacaaggac gacgacgaca agggcggatc aacaagtttg
     2881 tacaaaaaag caggcttagc caccatgccc aattcagaga gacatggggg caagaaggac
     2941 gggagtggag gagcttctgg aactttgcag ccgtcatcgg gaggcggcag ctctaacagc
     3001 agagagcgtc accgcttggt atcgaagcac aagcggcata agtccaaaca ctccaaagac
     3061 atggggttgg tgacccccga agcagcatcc ctgggcacag ttatcaaacc tttggtggag
     3121 tatgatgata tcagctctga ttccgacacc ttctccgatg acatggcctt caaactagac
     3181 cgaagggaga acgacgaacg tcgtggatca gatcggagcg accgcctgca caaacatcgt
     3241 caccaccagc acaggcgttc ccgggactta ctaaaagcta aacagaccga aaaagaaaaa
     3301 agccaagaag tctccagcaa gtcgggatcg atgaaggacc ggatatcggg aagttcaaag
     3361 cgttcgaatg aggagactga tgactatggg aaggcgcagg tagccaaaag cagcagcaag
     3421 gaatccaggt catccaagct ccacaaggag aagaccagga aagaacggga gctgaagtct
     3481 gggcacaaag accggagtaa aagtcatcga aaaagggaaa cacccaaaag ttacaaaaca
     3541 gtggacagcc caaaacggag atccaggagc ccccacagga agtggtctga cagctccaaa
     3601 caagatgata gcccctcggg agcttcttat ggccaagatt atgaccttag tccctcacga
     3661 tctcatacct cgagcaatta tgactcctac aagaaaagtc ctggaagtac ctcgagaagg
     3721 cagtcggtca gtccccctta caaggagcct tcggcctacc agtccagcac ccggtcaccg
     3781 agcccctaca gtaggcgaca gagatctgtc agtccctata gcaggagacg gtcgtccagc
     3841 tacgaaagaa gtggctctta cagcgggcga tcgcccagtc cctatggtcg aaggcggtcc
     3901 agcagccctt tcctgagcaa gcggtctctg agtcggagtc cactccccag taggaaatcc
     3961 atgaagtcca gaagtagaag tcctgcatat tcaagacatt catcttctca tagtaaaaag
     4021 aagagatcca gttcacgcag tcgtcattcc agtatctcac ctgtcaggct tccacttaat
     4081 tccagtctgg gagctgaact cagtaggaaa aagaaggaaa gagcagctgc tgctgctgca
     4141 gcaaagatgg atggaaagga gtccaagggt tcacctgtat ttttgcctag aaaagagaac
     4201 agttcagtag aggctaagga ttcaggtttg gagtctaaaa agttacccag aagtgtaaaa
     4261 ttggaaaaat ctgccccaga tactgaactg gtgaatgtaa cacatctaaa cacagaggta
     4321 aaaaattctt cagatacagg gaaagtaaag ttggatgaga actccgagaa gcatcttgtt
     4381 aaagatttga aagcacaggg aacaagagac tctaaaccca tagcactgaa agaggagatt
     4441 gttactccaa aggagacaga aacatcagaa aaggagaccc ctccacctct tcccacaatt
     4501 gcttctcccc caccccctct accaactact acccctccac ctcagacacc ccctttgcca
     4561 cctttgcctc caataccagc tcttccacag caaccacctc tgcctccttc tcagccagca
     4621 tttagtcagg ttcctgcttc cagtacttca actttgcccc cttctactca ctcaaagaca
     4681 tctgctgtgt cctctcaggc aaattctcag ccccctgtac aggtttctgt gaagactcaa
     4741 gtatctgtaa cagctgctat tccacacctg aaaacttcaa cgttgcctcc tttgcccctc
     4801 ccacccttat tacctggaga tgatgacatg gatagtccaa aagaaactct tccttcaaaa
     4861 cctgtgaaga aagagaagga acagaggaca cgtcacttac tcacagacct tcctctccct
     4921 ccagagctcc ctggtggaga tctgtctccc ccagactctc cagaaccaaa ggcaatcaca
     4981 ccacctcagc aaccatataa aaagagacca aaaatttgtt gtcctcgtta tggagaaaga
     5041 agacaaacag aaagcgactg ggggaaacgc tgtgtggaca agtttgacat tattgggatt
     5101 attggagaag gaacctatgg ccaagtatat aaagccaagg acaaagacac aggagaacta
     5161 gtggctctga agaaggtgag actagacaat gagaaagagg gcttcccaat cacagccatt
     5221 cgtgaaatca aaatccttcg tcagttaatc caccgaagtg ttgttaacat gaaggaaatt
     5281 gtcacagata aacaagatgc actggatttc aagaaggaca aaggtgcctt ttaccttgta
     5341 tttgagtata tggaccatga cttaatggga ctgctagaat ctggtttggt gcacttttct
     5401 gaggaccata tcaagtcgtt catgaaacag ctaatggaag gattggaata ctgtcacaaa
     5461 aagaatttcc tgcatcggga tattaagtgt tctaacattt tgctgaataa cagtgggcaa
     5521 atcaaactag cagattttgg acttgctcgg ctctataact ctgaagagag tcgcccttac
     5581 acaaacaaag tcattacttt gtggtaccga cctccagaac tactgctagg agaggaacgt
     5641 tacacaccag ccatagatgt ttggagctgt ggatgtattc ttggggaact attcacaaag
     5701 aagcctattt ttcaagccaa tctggaactg gctcagctag aactgatcag ccgactttgt
     5761 ggtagccctt gtccagctgt gtggcctgat gttatcaaac tgccctactt caacaccatg
     5821 aaaccgaaga agcaatatcg aaggcgtcta cgagaagaat tctctttcat tccttctgca
     5881 gcacttgatt tattggacca catgctgaca ctagatccta gtaagcggtg cacagctgaa
     5941 cagaccctac agagcgactt ccttaaagat gtcgaactca gcaaaatggc tcctccagac
     6001 ctcccccact ggcaggattg ccatgagttg tggagtaaga aacggcgacg tcagcgacaa
     6061 agtggtgttg tagtcgaaga gccacctcca tccaaaactt ctcgaaaaga aactacctca
     6121 gggacaagta ctgagcctgt gaagaacagc agcccagcac cacctcagcc tgctcctggc
     6181 aaggtggagt ctggggctgg ggatgcaata ggccttgctg acatcacaca acagctgaat
     6241 caaagtgaat tggcagtgtt attaaacctg ctgcagagcc aaaccgacct gagcatccct
     6301 caaatggcac agctgcttaa catccactcc aacccagaga tgcagcagca gctggaagcc
     6361 ctgaaccaat ccatcagtgc cctgacggaa gctacttccc agcagcagga ctcagagacc
     6421 atggccccag aggagtcttt gaaggaagca ccctctgccc cagtgatcct gccttcagca
     6481 gaacagacga cccttgaagc ttcaagcaca ccagctgaca tgcagaatat attggcagtt
     6541 ctcttgagtc agctgatgaa aacccaagag ccagcaggca gtctggagga aaacaacagt
     6601 gacaagaaca gtgggccaca ggggccccga agaactccca caatgccaca ggaggaggca
     6661 gcagcatgtc ctcctcacat tcttccacca gagaagaggc cccctgagcc ccccggacct
     6721 ccaccgccgc cacctccacc ccctctggtt gaaggcgatc tttccagcgc cccccaggag
     6781 ttgaacccag ccgtgacagc cgccttgctg caacttttat cccagcctga agcagagcct
     6841 cctggccacc tgccacatga gcaccaggcc ttgagaccaa tggagtactc cacccgaccc
     6901 cgtccaaaca ggacttatgg aaacactgat gggcctgaaa cagggttcag tgccattgac
     6961 actgatgaac gaaactctgg tccagccttg acagaatcct tggtccagac cctggtgaag
     7021 aacaggacct tctcaggctc tctgagccac cttggggagt ccagcagtta ccagggcaca
     7081 gggtcagtgc agtttccagg ggaccaggac ctccgttttg ccagggtccc cttagcgtta
     7141 cacccggtgg tcgggcaacc attcctgaag gctgagggaa gcagcaattc tgtggtacat
     7201 gcagagacca aattgcaaaa ctatggggag ctggggccag gaaccactgg ggccagcagc
     7261 tcaggagcag gccttcactg ggggggccca actcagtctt ctgcttatgg aaaactctat
     7321 cgggggccta caagagtccc accaagaggg ggaagaggga gaggagttcc ttactcaggc
     7381 tcgagcggca acccagcttt cttgtacaaa gtggttgatt aagatccact agtaacggcc
     7441 gccagtgtgc tggaattaat tcgctgtctg cgagggccag ctgttggggt gagtactccc
     7501 tctcaaaagc gggcatgact tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt
     7561 tgatattcac ctggcccgcg gtgatgcctt tgagggtggc cgcgtccatc tggtcagaaa
     7621 agacaatctt tttgttgtca agcttgaggt gtggcaggct tgagatctgg ccatacactt
     7681 gagtgacaat gacatccact ttgcctttct ctccacaggt gtccactccc aggtccaact
     7741 gcaggtcgag catgcatcta gggcggccaa ttccgcccct ctccctcccc cccccctaac
     7801 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gtgattttcc
     7861 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     7921 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     7981 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     8041 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     8101 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     8161 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     8221 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     8281 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     8341 cgatgataag cttgccacaa cccacaagga gacgaccttc catgaccgag tacaagccca
     8401 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
     8461 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
     8521 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     8581 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     8641 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     8701 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     8761 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     8821 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     8881 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag tgcccgaagg
     8941 accgcgcgac ctggtgcatg acccgcaagc ccggtgcctg atatcgatag atcctaatca
     9001 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     9061 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     9121 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     9181 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     9241 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     9301 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     9361 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     9421 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     9481 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     9541 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga
     9601 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     9661 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     9721 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     9781 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     9841 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     9901 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     9961 aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat
    10021 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
    10081 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    10141 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    10201 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    10261 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    10321 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    10381 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    10441 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    10501 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    10561 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    10621 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    10681 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    10741 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    10801 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    10861 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    10921 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    10981 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    11041 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    11101 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    11161 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    11221 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    11281 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    11341 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    11401 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    11461 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    11521 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    11581 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    11641 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    11701 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    11761 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    11821 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    11881 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    11941 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    12001 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa
    12061 gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
    12121 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc
    12181 gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    12241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    12301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt
    12361 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    12421 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
    12481 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
    12541 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
    12601 ag
//
