LOCUS       sequence-260394-        1198 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of CDK12 (human) tagged with N-terminal 
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1198)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-CDK12_IDG-K
FEATURES             Location/Qualifiers
     source          1..1198
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      127..136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             136..159
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    169..193
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atccaattcc acaccgcccc agtaaatcgc ccagctcgtt tagtgaccgt cagatcgcct
       61 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccggcggcca taccggtcag
      121 ctcgaggcca ccatggacta caaggacgac gacgacaagg gcggatcaac cagtttgtac
      181 aaaaaagcag gcttagccac catgcccaat tcacagagac atgggggcaa gaaggacggg
      241 agtggaggag cttctggaac tttgcagccg tcatcgggag gcggcagctc taacagcaga
      301 gagcgtcacc gcttggtatc gaagcacaag cggcataagt ccaaacactc caaagacatg
      361 gggttggtga cccccgaagc agcatccctg ggcacagtta tcaaaccttt ggtggagtat
      421 gatgatatca gctctgattc cgacaccttc tccgatgaca tggccttcaa actagaccga
      481 agggagaacg acgaacgtcg tggatcagat cggagcgacc gcctgcacaa acatcgtcac
      541 caccagcaca ggcgttcccg ggacttacta aaagctaaac agaccgaaaa agaaaaaagc
      601 caagaagtct ccagcaagtc gggatcgatg aaggaccgga tatcgggaag ttcaaagcgt
      661 tcgaatgagg agactgatga ctatgggaag gcgcaggtag ccaaaagcag cagcaaggaa
      721 tccaggtcat ccaagctcca caaggagaag accaggaaag aacgggagct gaactctggg
      781 cacaaagacc ggagtaaaag tcatcgaaaa agggaaacac ccaaaagtta caaaacagtg
      841 gacagcccaa aacggagatc caggagcccc cacaggaagt ggtctgacag ctccaaacaa
      901 gatgatagcc cctcgggagc ttcttatggc caagaatatg accttagtcc ctcacgatct
      961 catacctcga gcattatgac tcctacaaaa aagtctggaa gtacttgaga aggtgtcggt
     1021 cagtcccctt aacaggagct ttcgggctac cagtctagca cccggtcacg gacccctaac
     1081 gtaggcgaat gaagatcgtc gttccctata ccagaaaacg gtcgtcccac ttccacagaa
     1141 gtggtccttt acagtggggc gattccccat tcttatgggt taaggagcgt cacagcta
//
