LOCUS       sequence-260396-        4239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC26A10
FEATURES             Location/Qualifiers
     source          1..4239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2364..2463)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2480..2499)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2481..2499)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2504..2520)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2504..2520)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2622..2641)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2633..3442
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2700..2719)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3589..4177
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4078..4097
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgcgcc tggacctggc ctccctgatg tctgccccaa agagcctggg
      721 ctccgccttc aagtcttgga ggctggataa ggcaccaagc ccacagcaca ccttcccatc
      781 cacatctatc ccaggaatgg cctttgccct gctggcaagc gtgccacccg tgttcggcct
      841 gtacacctcc ttctttcccg tgctgatcta ttctctgctg ggaacaggcc gccacctgtc
      901 caccggcaca tttgccatcc tgtctctgat gaccggaagc gccgtggagc ggctggtgcc
      961 tgagccactg gtgggcaacc tgtctggcat cgagaaggag cagctggacg cacagagggt
     1021 gggagtggca gcagccgtgg cctttggctc cggcgccctg atgctgggca tgttcgtgct
     1081 gcagctgggc gtgctgtcca cctttctgtc tgagcccgtg gtgaaggccc tgacatccgg
     1141 agccgccctg cacgtgctgc tgagccagct gccatccctg ctgggcctgt ctctgcccag
     1201 acagatcggc tgcttctccc tgtttaagac cctggcctct ctgctgacag ccctgccaag
     1261 gagctcccct gcagagctga ccatctctgc cctgagcctg gccctgctgg tgcctgtgaa
     1321 ggagctgaac gtgcggttca gagacaggct gcccacacct atcccaggag aggtggtgct
     1381 ggtgctgctg gcaagcgtgc tgtgcttcac ctctagcgtg gatacaagat accaggtgca
     1441 gatcgtggga ctgctgccag gaggatttcc acagcctctg ctgcctaatc tggccgagct
     1501 gccaaggatc ctggccgaca gcctgcctat cgccctggtg tccttcgccg tgagcgcctc
     1561 cctggcaagc atccacgccg acaagtactc ctataccatc gattctaacc aggagtttct
     1621 ggcccacggc gcctccaatc tgatctcctc tctgttcagc tgctttccca actccgccac
     1681 cctggccacc acaaatctgc tggtggatgc aggaggcaag acccagctgg caggactgtt
     1741 ttcttgtaca gtggtgctga gcgtgctgct gtggctggga cccttctttt actatctgcc
     1801 taaggccgtg ctggcctgca tcaacatcag ctccatgcgc caggtgttct gtcagatgca
     1861 ggagctgcct cagctgtggc acatctctcg ggtggacttt ctgctgcagg tgccaggcct
     1921 gtgcatcctg agctacccaa cccccctgta tttcggcaca agaggccagt ttaggtgtaa
     1981 tctggagtgg cacctgggac tgggagaggg agagaaggag accagcaagc ctgatggacc
     2041 aatggtggca gtggcagagc ctgtgagagt ggtggtgctg gacttctccg gagtgacatt
     2101 tgccgatgca gcaggagcaa gggaggtggt gcaggtgcgc gagcggctgg caagcagatg
     2161 cagggacgca aggatcaggc tgctgctggc acagtgtaat gccctggtgc agggcaccct
     2221 gacaagagtg ggcctgctgg acagggtgac cccagatcag ctgttcgtga gcgtgcagga
     2281 tgcagcagca tatgcactgg gatccctgct gaggggatct agcacacggt ctggcagcca
     2341 ggaggccctg ggctgcggca aggacccagc tttcttgtac aaagttggca ttataagaaa
     2401 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     2461 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     2521 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     2581 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     2641 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     2701 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
     2761 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     2821 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     2881 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac
     2941 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     3001 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     3061 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     3121 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
     3181 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     3241 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     3301 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     3361 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     3421 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
     3481 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc
     3541 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3601 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3661 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3721 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3781 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3841 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3901 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3961 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4021 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4081 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4141 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4201 acggttcctg gccttttgct ggccttttgc tcacatgtt
//
