LOCUS       sequence-260437-        1158 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  entry vector for GreenGate cloning method, promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pSW180a - pOp6
FEATURES             Location/Qualifiers
     source          1..1158
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    98..119
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(134..139,140..157,158..164)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    172..188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        202..220
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     202..219
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    292..311
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    344..363
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    396..415
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     gap             978..988
                     /estimated_length=11
     gap             996..1011
                     /estimated_length=16
     gap             1029..1046
                     /estimated_length=18
     gap             1049..1063
                     /estimated_length=15
     gap             1075..1084
                     /estimated_length=10
     gap             1088..1140
                     /estimated_length=53
ORIGIN
        1 anncgcnnnc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
       61 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      121 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      181 gataacaatt tcacacgagc tatttaggtg acactataga agtgaagctt ggtctcaacc
      241 tatgcatatg tcgagctcaa gaaatctaga aagaagaaag ggaagagaaa gaattgtgag
      301 cgctcacaat tgaaagacta gaaagaagaa agggaagaga aagaattgtg agcgctcaca
      361 attgaaagac tagaaagaag aaagggaaga gaaagaattg tgagcgctca caattgaaag
      421 actagagaga cgatctgcaa gagacccaat tgtgagcgct cgcaattgat ttactannng
      481 gacgaccttc nagagacccn nccctgagcc aacggtgnga tttactanct ggacgacntt
      541 gnannncnnc ctgactggga cnacngtgnn ntacccaact gnncgcnttg nacaacntcc
      601 ccattgnnac nctgngggaa tagcgaacag nnccgnattg atcaacntcc ccattggtnt
      661 cgctgcnnga atggcnaacg nnnccggatn nntcatnntc cccntnctnt nnntgcngga
      721 attgannncg gncncggngc gctcnnnntc cnnttgactc tgnctgnggg nnttgacngn
      781 gccnnnnnnc nctcnnacac cagctgannc tgnctgccgg gantgnntgn nnccngnggn
      841 nctctncana ccagctgaca ccnnnngcan nnanngnntn nngnnnnnnn tntcttnann
      901 nncannngnn nnnnnnnang gnnnnntnnn nnnnnngntn nnttnnnnag nnanngnnnn
      961 nnnnnannnn nnngnntnnn nnnnnnnngn antttnnnnn nnnnnnnnnn nannannnnn
     1021 nttnnntgnn nnnnnnnnnn nnnnnnaann nnnnnnnnnn nnngannncn nnnannnnnn
     1081 nnnntncnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 tncnnnntan nnnnannt
//
