LOCUS       sequence-260447-        4446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC6A11
FEATURES             Location/Qualifiers
     source          1..4446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2571..2670)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2687..2706)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2688..2706)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2711..2727)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2711..2727)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2829..2848)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2840..3649
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2907..2926)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3796..4384
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4285..4304
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgaccg cagagaaggc cctgccactg ggaaatggca aggcagcaga
      721 ggaggcaaga gagagcgagg cccctggagg aggatgcagc tccggaggag cagcacctgc
      781 ccggcaccca agagtgaaga gggataaggc cgtgcacgag cgcggccact ggaacaataa
      841 ggtggagttc gtgctgtccg tggcaggaga gatcatcgga ctgggcaacg tgtggcggtt
      901 cccctacctg tgctataaga acggcggcgg cgcctttctg atcccatacg tggtgttctt
      961 tatctgctgt ggcatccccg tgttctttct ggagaccgcc ctgggacagt tcacaagcga
     1021 gggaggcatc acctgctgga ggaaggtgtg ccctctgttt gagggcatcg gctacgccac
     1081 ccaggtcatc gaggcccacc tgaacgtgta ctatatcatc atcctggcct gggccatctt
     1141 ctatctgtcc aactgcttta ccacagagct gccatgggcc acatgcggcc acgagtggaa
     1201 taccgagaac tgcgtggagt tccagaagct gaacgtgagc aactactctc acgtgagcct
     1261 gcagaatgcc acaagccccg tgatggagtt ttgggagcac cgcgtgctgg ccatctccga
     1321 tggcatcgag cacatcggaa acctgagatg ggagctggcc ctgtgcctgc tggccgcctg
     1381 gaccatctgc tacttctgta tctggaaggg caccaagtcc acaggcaagg tggtgtacgt
     1441 gaccgccaca tttccttata tcatgctgct gatcctgctg atcagaggag tgacactgcc
     1501 aggagcatct gagggcatca agttctacct gtatcccgac ctgtccaggc tgtctgatcc
     1561 tcaagtgtgg gtggacgccg gcacacagat cttcttttcc tacgccatct gcctgggatg
     1621 tctgaccgcc ctgggatctt acaacaacta caacaacaac tgctacagag actgtatcat
     1681 gctgtgctgt ctgaatagcg gcacctcctt cgtggcagga ttcgcaatct tttccgtgct
     1741 gggctttatg gcctacgagc agggagtgcc aatcgcagag gtggcagagt ctggaccagg
     1801 actggccttt atcgcctatc ccaaggccgt gacaatgatg ccactgagcc ccctgtgggc
     1861 caccctgttc tttatgatgc tgatcttcct gggcctggat agccagttcg tgtgcgtgga
     1921 gtccctggtg acagccgtgg tggacatgta ccctaaggtg ttccggagag gctataggcg
     1981 cgagctgctg atcctggccc tgtctgtgat cagctacttt ctgggactgg tcatgctgac
     2041 cgagggagga atgtacatct tccagctgtt tgactcctat gccgcctctg gcatgtgcct
     2101 gctgttcgtg gccatctttg agtgcatctg tatcggctgg gtgtacggct ctaatagatt
     2161 ctatgacaac atcgaggata tgatcggcta caggccccct agcctgatca agtggtgctg
     2221 gatgatcatg acacccggca tctgtgccgg catctttatc ttctttctga tcaagtacaa
     2281 gcccctgaag tataataaca tctacaccta tcctgcctgg ggctatggca tcggctggct
     2341 gatggccctg tctagcatgc tgtgcatccc tctgtggatc tgtatcacag tgtggaagac
     2401 cgagggcaca ctgcctgaga agctgcagaa gctgaccaca ccatctaccg atctgaagat
     2461 gaggggcaag ctgggcgtga gcccacggat ggtgacagtg aacgactgcg atgccaagct
     2521 gaagagcgac ggcaccatcg ccgccatcac cgagaaggag acacacttcg acccagcttt
     2581 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     2641 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     2701 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     2761 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     2821 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     2881 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     2941 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     3001 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     3061 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     3121 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     3181 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     3241 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     3301 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     3361 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     3421 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     3481 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     3541 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     3601 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     3661 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
     3721 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     3781 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3841 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3901 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3961 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4021 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4081 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4141 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4201 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4261 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4321 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4381 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4441 catgtt
//
