LOCUS       sequence-260448-       10644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid with pLL1270 as backbone and alpha-Xylp_1211 and 
            alpha-L-Araf_996 as inserts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10644)
  TITLE     Expression of arabinoxylan debranching enzymes in 
            Thermoanaerobacterium thermosaccharolyticum- Bacterial expression 
            plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLL1274
FEATURES             Location/Qualifiers
     source          1..10644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     95..114
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     308..330
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     322..339
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     323..339
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             1169..1177
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(2899..3693)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      8416..8443
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(8461..8477)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8461..8477)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8474..8496)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8485..8501
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8509..8515,8516..8533,8534..8539))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8554..8575
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8769..9357)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8849..8868)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9528..10319,10320..10388))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10151..10170
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10389..10493)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10561..10579
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10617..10639)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
       61 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
      121 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
      181 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
      241 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
      301 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgacaaa gttttctatt
      361 tgtgttaaca ttgtttatat aatagtgaac agtgttaaga ttaaatgtga ggtgtttgta
      421 tggatattaa tgattataaa gagaagggac tttatttatt aagtagtatg gatgatttta
      481 ttaaaattaa tgatttgttt atgggtaaag ttgtttctcc tggctatgtt gcttcggttt
      541 ttggtgtttc caggtctact gttacacaat ggattcaaag acgtaaaatt agagctttta
      601 agtataaagg taaggaaggt gactatatgg ttatacctat tgctgatatt attgattaca
      661 aaagattgag taataatgat tttatttatg ataagttagt gaggtgattt attttatgtt
      721 tgacgatagc tatgttgtta atgagtgttc gtctaatgtt agtgaaaatg atagagattt
      781 ttgtagtttg gttggtcgtt ttatgattat taatggtata gataagttgg ttattaagat
      841 taatagaaaa tttaatagga aatctttaag tttagatttt agtgttgatt tattcccttc
      901 tatcaaagtt tctgaattag ttttttttga tgagtttaac aaaacgtgtg gtttttattt
      961 ttcttttaat tcttttacaa tttttaaggc ttttagagat gttcataatc ataataaaat
     1021 atcattttat tttgcataat ttcgggtctg ggccgcagac caggcccagt gctaacaata
     1081 ttaattttta atgttaggaa ttgtttaatt cttaattgtg tttttaaagg tagaataatt
     1141 acccattcgc cctttagcca acaaaaatta aggaggtata aacatggata aaatggattt
     1201 gattcttcaa gatgaaagac tgggtgagat atttaaagat atagatttaa cagataatga
     1261 aaagagatat cttaaatggt tatggaaatg ggattatgaa acacgtgata cttttgtatc
     1321 aatttttttg aagctaaaaa atggtggaaa atgatttttt tcttatcttg atatattaga
     1381 aaaaagcgta ctcacgaagt aagaatttgt aaaaaaagaa ggggggattt ttttggatga
     1441 gagtttgtac aagcagattt taagtaatat tattattact cgtgattatt gtaaaaatgt
     1501 tttagataat ataaagttca atgaaaaaat aattgattat tatgttatgt tacaaaatga
     1561 tgtttttatt gattttacta ataaaataaa ttcaataagg gattgtaata aatattggta
     1621 tttggatgtt tataaaaagc agaaaataaa ggattttaaa aagactaatt tgtgtaaaga
     1681 taagttctgt aataattgta agaaagttaa acaggcttca agaatgcaaa aatatattcc
     1741 tgaattacag aaatacaaag atggcttata tcattttata tttactgttg aaaatgtgcc
     1801 aggtagtgaa ttaagagata ctattgatag gttgtttaag tcttttaagt catttacaag
     1861 gtatttaagt ggtaatctta aaataaaagg tgttaatttt gataaatggg gttataaagg
     1921 ctgtgtaagg tctttagagg taacttatag tatgattgat aatcatatta tgtatcatcc
     1981 acacttgcat gttgcgatga tattagatcc tttttacgat ggttttaatg ttgaaaggat
     2041 gcatataatt aataagttta gttatagcta tggtgtttta aaaaggttgt ttactgatga
     2101 tgaattatta attcaaaaaa tttggtattt attgtttaat aatattgagg ttaacatggc
     2161 caatataaat aatttagagg atggttattc ttgtttagtt aataagttta gtgattatga
     2221 ttatgcggag ctgtttaagt atatttgtaa aaatactgat gaacaaggtt tacttatgac
     2281 ttatgatatt tttaaagatt tatattttgc attacataat gttcatcaga tacaaggcta
     2341 tggttgttta tataatataa gagatgatac tcaattagat ttaaaggttg atgacattta
     2401 taatgatttg attgatttat tacaagttac agaaaatcct atacagtcta tggaaactgt
     2461 acaggattta ttaaaggata ctgaatatac aataataagc cgtaagcgta tatttaagta
     2521 tctaacacaa ttatatcata aggattgata tttataccgt ctgtcggact catgcggagg
     2581 gggacttgag ggggtctccc ctcgcattgt acgacagacg gtattattat tatacaaatt
     2641 ttttttatgt aatttttttt gtgtaatttt tttatacaaa taatatttca attcgagctc
     2701 ggtacccggg gatcctctag agtcgacctg caggcatgca cgatacaaat tcctcgtagg
     2761 cgctcgggac ccctatctag cgaactttta gaaaagatat aaaacatcag agtatggaca
     2821 gttgcggatg tacttcagaa aagattagat gtctaaaaag cttgtagtta aagcttttta
     2881 gacatctaaa tctaggtgct aaaacaattc atccagtaaa atataatatt ttattttctc
     2941 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
     3001 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
     3061 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
     3121 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
     3181 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
     3241 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
     3301 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
     3361 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
     3421 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
     3481 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
     3541 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
     3601 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
     3661 tttcaattcc ggtgatattc tcattttagc catttattat ttccttcccc ttttctacag
     3721 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt
     3781 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg
     3841 tataacatag tatcgacgga gccgattttg aaaccacaat tatgatagaa tttacaagct
     3901 ataaggttat tgtcctgggt ttcaagcatt agtccatgca agtttttatg ctttgcccat
     3961 tctatagata tattgataag cgcgctgcct atgccttgcc ccctgaaatc cttacatacg
     4021 gcgatatctt ctatataaaa gatatattat cttatcagta ttgtcaatat attcaaggca
     4081 atctgcctcc tcatcctctt catcctcttc gtcttggtag ctttttaaat atggcgcttc
     4141 atagagtaat tctgtaaagg tccaattctc gttttcatac ctcggtataa tcttacctat
     4201 cacctcaaat ggttcgctgg gtttgagtcg tgactaagaa cgtcaaagta attaacaata
     4261 cagctatttt tctcatgctt ttaccccttt cataaaattt aattttatcg ttatcataaa
     4321 aaattataga cgttatattg cttgccggga tatagtgctg ggcattcgtt ggtgcaaaat
     4381 gttcggagta aggtggatat tgatttgcat attgatctat tgcattgaaa tgattagtta
     4441 tccgtaaata ttaattaatc atatcataaa ttaattatat cataattgtt ttgacgaatg
     4501 aaggtttttg gataaattat caagtaaagg aacgctaaaa attttggcgt aaaatatcaa
     4561 aatgaccact tgaattaata tggtaaagta gatataatat tttggtaaac atgccttcag
     4621 caaggttaga ttagctgttt ccgtataaat taaccgtatg gtaaaacggc agtcagaaaa
     4681 ataagtcata agattccgtt atgaaaatat acttcggtag ttaataataa gagatatgag
     4741 gtgagagata caagataaga gatataaggt acgaatgtat aagatggtgc ttttaggcac
     4801 actaaataaa aaacaaataa acgaaaattt taaggaggac gaaagatggc aaagataacg
     4861 attctggaag acaagataat ttttgaaaga agagatgagc ttacggtaat agaagcttat
     4921 ggagcaaatt gtctgcgtta ccgttcaacc cgcaatatta agctgtctga tgagaactgg
     4981 accctgctgc cgccgactac aaaagctgat tgtaaaacct ttggaaatga aagagaagca
     5041 acgatagtaa acggcgatat ttccgcaact ataagggacg gctttggctg gaccggagcc
     5101 agtataagct actttcgcaa ggggaagcag attcttcgat cccgtgatga gggtgatccg
     5161 gttacccgct ttacccacct gggcggtaac aattatcagg tgaaggcgat atttgacagt
     5221 aatccgggcg agcatatata tggcctggga caggagcagg aggatcagtt tgaccgcaag
     5281 ggctgcacct gcaatatcat gcattacaat accaagtcga ctttgccggt tatttattcg
     5341 tctctgggat atggcttttt gtggaacaat cctgcacccg gccgttgtga gactaccttt
     5401 aatcatacaa tgtgggttgc ggatagtgca tatcagattg attatcttgt atatgtcggg
     5461 gatactccgg ctgacataat gaaaatatac tgtgatctga caggctatgc cccgaaattc
     5521 cctgactggg cggccggctt ctggcagtca aagctccgtt atgaaagcca ggaggatttg
     5581 ctggaggtgg caagggaata caagaaaaga ggaattcctc tcgcagcgat agtaatagat
     5641 tatttccact ggacggagca gggggaatgg aaatttgatc ccaatttgtg gcctgatccc
     5701 aaggcaatgt gtgacgagct tgagtcaatg ggagtaaagc ccgttgtatc catctggcct
     5761 accattaatc ccaagagtga gaattatcat gcaatggata atgagaatat gctggtgagg
     5821 accgagaacg ggcagtatgg catattcaac ttttacggct tacagacctt tatagatccc
     5881 accaatccta aaacagggcc ctttgtatgg agcaaggtaa gggagaatta ttataagtac
     5941 ggaattaaga acttttggct ggatgaggct gagccggagg tgcatcctca gcagtttggc
     6001 aatctgcgtt tttatatagg taacggagcc cagacggcat tattgtatcc atattattat
     6061 gcaaagatat tttatgatgg cttaagaagt gagggagaag aggatattat aatccttacc
     6121 cgggcagcct atcccggcag ccaaagattt ggttccctgg tatggaacgg agacatcccc
     6181 tccaccttca aggctcttcg ccagagtgta accagcggcc tttccatgtc aatgtgcgga
     6241 atcccatggt ggaacagcga cataggcggc ttccattccg cagatataga aagcgactat
     6301 ttcagggagc ttatagtcag atggttccag ttcggcctct ttagtccggt tatgcgcctt
     6361 cacggggcca gaaaccgtac aaaaaaccag gttgaccgcc atccgggcgt gaaggagaga
     6421 agcggcggag acaatgaaat ctggagcttc ggtgaaagaa attatcctat tcttaagaat
     6481 attatagagc ttagggagag gctgaagcct tatattctca aatatatgga tatcgccagc
     6541 aagaccggct caccgattat gaggccgatg ttctatgatt tttatgatga cgagatttgc
     6601 taccgacttg atgatcagta catgttcgga gaggatatac tctttgctcc cattctggag
     6661 cagggagtga cacagcgcag tgtctacctg ccggagggta aatgggttaa tgtaaatgat
     6721 aaaaaaacct acgagggcag gcaaatgata actgcaaggg cagagctgca tgagtttatt
     6781 gccttcgtaa gaagcggcag cgaggtgctg gaggtattta gctaattcta aataggaggt
     6841 gtaaagtatg aaaaagcaag gctttaaccc ttatcttcct tcctgggaat atatccctga
     6901 tggagaaccc tatgtattta atgacagagt ctatgtatat ggctcccatg accgtttcaa
     6961 tggatacgca ttctgcctga atgattatgt ttgctggtct gctcctgttg acaacctggc
     7021 tgattggcgc tatgaaggtg tcatattaag agctgaggat gtagagaaca atgatgaccg
     7081 cgacagctgc ctttatgctc ctgatgttac ggtaggtccg gacggaagat attacttata
     7141 ttatgttgac agcaggcgtt caatagtatc tgttgcagtt agtgatactc ctgcaggata
     7201 ttataatttt tatggatatg ttcattatcc cgacggaaca cgtctgggtg agcgggaagg
     7261 cgacgagcca caatttgatc cggctgttct cacagagggt gacaaaacct atctctatac
     7321 aggcttctgc gcaagaggag acaaatccag acacggtgcc atggcaaccg tacttggtcc
     7381 ggatatgctg actattatag aagaaccggt atttatcgta cccagccagc catacagtga
     7441 aggcacaggc tatgagggac atgaattctt tgaagctcct tccatcagga agcgtggaga
     7501 tacatattac tttatatact cctcaattgt aatgcatgag ctgtgttatg ccaccagcaa
     7561 gcatccgacc aaggatttca aatacggcgg tgttatcgtt agcaactgcg atctccatat
     7621 cgacagctac aagcctgcca atatgatggc ttactaccct ggcaacaatc acggaagcat
     7681 agtacagatt aaggacaaat ggtatatctt ctaccacaga catactaacg gaacccagta
     7741 cagcagacag ggctgcatgg aggaaatcaa attcctcgag gacggctcta tacctcaggt
     7801 tgaaataacc tcctgcggct caaacggcgg tcctctggag ggtaaaggcg aatatgccac
     7861 ttatattgca tgcaatctct tccttaagga agatcctaag cttcccggca acggcagatg
     7921 gcctatgttc tataatccat atccttatat tacccaggat ggcaaggatg gcgacgagga
     7981 gttgggttac attgccaaca tgcaatattc cgctacagca ggcttcaagt attttgactg
     8041 caagggaatt aagaagataa agattaaggt tcgcggatat tgccgcggtg actttgaggt
     8101 aagaaccaaa tgggacggcc cggtactagg tacaatcccg gtagtattct ccaactgctg
     8161 gaaggaatac agtgccgata tagctattcc ggacggcatt aacgccctct acttcaccta
     8221 tgcaggaaca ggaagcgcca gctttgcttc ctttacactg gaataactgg gcctttcgtt
     8281 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
     8341 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
     8401 ggcatcaaat taagcagaag gccatcctga cggatggcct tttagcttgg cgtaatcatg
     8461 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8521 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8581 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcaga tctgcggcga
     8641 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8701 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8761 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8821 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8881 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8941 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     9001 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     9061 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9121 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9181 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9241 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9301 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9361 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9421 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9481 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9541 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     9601 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9661 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9721 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9781 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9841 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9901 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9961 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10021 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10081 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10141 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10201 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10261 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10321 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10381 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10441 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10501 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
    10561 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
    10621 tgacacatgc agctcccgga gacg
//
