LOCUS       sequence-260451-       11028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid with pLL1270 as backbone and alpha-Xylp_1211 and 
            alpha-L-Araf_1120 as inserts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11028)
  TITLE     Expression of arabinoxylan debranching enzymes in 
            Thermoanaerobacterium thermosaccharolyticum- Bacterial expression 
            plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLL1272
FEATURES             Location/Qualifiers
     source          1..11028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      4779..4806
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(4824..4840)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4824..4840)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4837..4859)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4848..4864
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4872..4878,4879..4896,4897..4902))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4917..4938
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5132..5720)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5212..5231)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5891..6682,6683..6751))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6514..6533
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6752..6856)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6924..6942
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6980..7002)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7102..7121
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7315..7337
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7329..7346
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7330..7346
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             8176..8184
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(9906..10700)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 cgatatcttc tatataaaag atatattatc ttatcagtat tgtcaatata ttcaaggcaa
       61 tctgcctcct catcctcttc atcctcttcg tcttggtagc tttttaaata tggcgcttca
      121 tagagtaatt ctgtaaaggt ccaattctcg ttttcatacc tcggtataat cttacctatc
      181 acctcaaatg gttcgctggg tttgagtcgt gactaagaac gtcaaagtaa ttaacaatac
      241 agctattttt ctcatgcttt tacccctttc ataaaattta attttatcgt tatcataaaa
      301 aattatagac gttatattgc ttgccgggat atagtgctgg gcattcgttg gtgcaaaatg
      361 ttcggagtaa ggtggatatt gatttgcata ttgatctatt gcattgaaat gattagttat
      421 ccgtaaatat taattaatca tatcataaat taattatatc ataattgttt tgacgaatga
      481 aggtttttgg ataaattatc aagtaaagga acgctaaaaa ttttggcgta aaatatcaaa
      541 atgaccactt gaattaatat ggtaaagtag atataatatt ttggtaaaca tgccttcagc
      601 aaggttagat tagctgtttc cgtataaatt aaccgtatgg taaaacggca gtcagaaaaa
      661 taagtcataa gattccgtta tgaaaatata cttcggtagt taataataag agatatgagg
      721 tgagagatac aagataagag atataaggta cgaatgtata agatggtgct tttaggcaca
      781 ctaaataaaa aacaaataaa cgaaaatttt aaggaggacg aaagatggca aagataacga
      841 ttctggaaga caagataatt tttgaaagaa gagatgagct tacggtaata gaagcttatg
      901 gagcaaattg tctgcgttac cgttcaaccc gcaatattaa gctgtctgat gagaactgga
      961 ccctgctgcc gccgactaca aaagctgatt gtaaaacctt tggaaatgaa agagaagcaa
     1021 cgatagtaaa cggcgatatt tccgcaacta taagggacgg ctttggctgg accggagcca
     1081 gtataagcta ctttcgcaag gggaagcaga ttcttcgatc ccgtgatgag ggtgatccgg
     1141 ttacccgctt tacccacctg ggcggtaaca attatcaggt gaaggcgata tttgacagta
     1201 atccgggcga gcatatatat ggcctgggac aggagcagga ggatcagttt gaccgcaagg
     1261 gctgcacctg caatatcatg cattacaata ccaagtcgac tttgccggtt atttattcgt
     1321 ctctgggata tggctttttg tggaacaatc ctgcacccgg ccgttgtgag actaccttta
     1381 atcatacaat gtgggttgcg gatagtgcat atcagattga ttatcttgta tatgtcgggg
     1441 atactccggc tgacataatg aaaatatact gtgatctgac aggctatgcc ccgaaattcc
     1501 ctgactgggc ggccggcttc tggcagtcaa agctccgtta tgaaagccag gaggatttgc
     1561 tggaggtggc aagggaatac aagaaaagag gaattcctct cgcagcgata gtaatagatt
     1621 atttccactg gacggagcag ggggaatgga aatttgatcc caatttgtgg cctgatccca
     1681 aggcaatgtg tgacgagctt gagtcaatgg gagtaaagcc cgttgtatcc atctggccta
     1741 ccattaatcc caagagtgag aattatcatg caatggataa tgagaatatg ctggtgagga
     1801 ccgagaacgg gcagtatggc atattcaact tttacggctt acagaccttt atagatccca
     1861 ccaatcctaa aacagggccc tttgtatgga gcaaggtaag ggagaattat tataagtacg
     1921 gaattaagaa cttttggctg gatgaggctg agccggaggt gcatcctcag cagtttggca
     1981 atctgcgttt ttatataggt aacggagccc agacggcatt attgtatcca tattattatg
     2041 caaagatatt ttatgatggc ttaagaagtg agggagaaga ggatattata atccttaccc
     2101 gggcagccta tcccggcagc caaagatttg gttccctggt atggaacgga gacatcccct
     2161 ccaccttcaa ggctcttcgc cagagtgtaa ccagcggcct ttccatgtca atgtgcggaa
     2221 tcccatggtg gaacagcgac ataggcggct tccattccgc agatatagaa agcgactatt
     2281 tcagggagct tatagtcaga tggttccagt tcggcctctt tagtccggtt atgcgccttc
     2341 acggggccag aaaccgtaca aaaaaccagg ttgaccgcca tccgggcgtg aaggagagaa
     2401 gcggcggaga caatgaaatc tggagcttcg gtgaaagaaa ttatcctatt cttaagaata
     2461 ttatagagct tagggagagg ctgaagcctt atattctcaa atatatggat atcgccagca
     2521 agaccggctc accgattatg aggccgatgt tctatgattt ttatgatgac gagatttgct
     2581 accgacttga tgatcagtac atgttcggag aggatatact ctttgctccc attctggagc
     2641 agggagtgac acagcgcagt gtctacctgc cggagggtaa atgggttaat gtaaatgata
     2701 aaaaaaccta cgagggcagg caaatgataa ctgcaagggc agagctgcat gagtttattg
     2761 ccttcgtaag aagcggcagc gaggtgctgg aggtatttag ctaattctaa ataggaggtg
     2821 taaagtatga tgaaggaaga tagatatgtg ctgtgctata cacgagagcc acaggaggat
     2881 attatttatt ccaacaagct ggcttacagt atgcatctgg catatagcga aaatggagta
     2941 aaatatgagc cacttaacca caattctgga gttttgtttg caaaagccac tcataatccg
     3001 gatggtacga tgacagcaaa aagcctgaag tgcccgtttt tgttttacat ggaggacaat
     3061 acctttggtg ttgttgcaat aaggacggag gctaacggag attatgatcc ggaatgcagg
     3121 ggcaaggtat tgttctttac ctcaaaggat ttgattcagt acaccgaggt tggcctgata
     3181 gatcttaata cgaatgcata tatcagggat gttacctgta aatatgacaa ggttaaggaa
     3241 agatacataa tatgctggcg tgacgattac ggtaagtggt atcagagtga aatagatgat
     3301 ttattttctc ttaagtgcca gtctgaaccc gtcagcatag gagcagttgg ctttgccagg
     3361 gttaatactg aaatagaagg aatagtaccc cgcaatacca taaaggtatc tgaagaaatc
     3421 ggatacagat taaagagcaa gctgacaaca ccccgcatgg tcaaaatcga gctgcctgaa
     3481 accttaagtg cttcttcacc tgaagagctg agcaaaatga gatttaaagc catttacagt
     3541 gacggtacca gctcgatcaa gtctatagac tggaatactg agagggttga ttggagcagg
     3601 cccggcacct atgatataaa gggtgtaatt catcaggatc attttgaatt ccccattgcc
     3661 atcaaccgtg ctgatccctg catccgcaaa tggaatggca agtattattt tgtggcaact
     3721 aatgacgccg acggcaatca caccatatat atcagggaag ctgatactat tccgggactt
     3781 gcaactgctg aggagcactt aattcttgac tcctccacct atgaaggtat cggtggtctg
     3841 ttatgggctc cggagcttca tgttatagga gaggatttgt atatcttcca tgcagcaaca
     3901 ccgggagaat tcttccatga ggaatcccat gtaatgcaac tgaagcgtgg cggcaatcct
     3961 ctttgcaagg aggattggtc ggcaccgaag agagtagtta aaaaggatgg ttcttacctg
     4021 tgtgaggccg gcaaggttat atcacttgat atgacctgct ttgagtggaa taaggaatac
     4081 tatgttgcct ggtcacaaag acagttcctg ccagttgatc agggagcttg gctctatata
     4141 gccaaaattg atcctcagga gccatggaga ttaatcaccg atcctgttct gctcagcaag
     4201 cctgaatatg gttgggcaaa taatcatacc tttgttgatg aaggaccttt tgccctgata
     4261 acagataaaa aaatctttct taccttctcc agcgctgcgg ttgatgccac ctatacagtt
     4321 ggcctgctgg tggctgagcc gggttctgat ttgctggatc ccgcaagctg gacaaagctg
     4381 aattatcctt tactgaccag cagaagcgta cctggtcaat tcggaccggg acataactcc
     4441 tatgtaattg atgatgatgg cattgtatgg aatgcttacc atgccagacc aggagttaac
     4501 ggtcccagaa gctcaggaat aagacgcgtt cactttgaca tagacggata cccggtgctt
     4561 gatcttactg aggaaagaga cgtaagcgcc cgtctgaaga atgtatcctg caggcttata
     4621 gtaaaatagc tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
     4681 gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc
     4741 aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg
     4801 ccttttagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     4861 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     4921 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     4981 tcgtgccagc agatctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5041 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5101 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5161 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5221 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5281 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5341 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5401 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5461 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5521 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     5581 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5641 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5701 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5761 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5821 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     5881 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5941 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6001 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6061 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6121 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6181 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     6241 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     6301 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     6361 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6421 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6481 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     6541 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6601 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6661 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6721 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6781 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6841 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     6901 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     6961 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     7021 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     7081 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     7141 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
     7201 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     7261 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     7321 cacgacgttg taaaacgacg gccagtgaat tgacaaagtt ttctatttgt gttaacattg
     7381 tttatataat agtgaacagt gttaagatta aatgtgaggt gtttgtatgg atattaatga
     7441 ttataaagag aagggacttt atttattaag tagtatggat gattttatta aaattaatga
     7501 tttgtttatg ggtaaagttg tttctcctgg ctatgttgct tcggtttttg gtgtttccag
     7561 gtctactgtt acacaatgga ttcaaagacg taaaattaga gcttttaagt ataaaggtaa
     7621 ggaaggtgac tatatggtta tacctattgc tgatattatt gattacaaaa gattgagtaa
     7681 taatgatttt atttatgata agttagtgag gtgatttatt ttatgtttga cgatagctat
     7741 gttgttaatg agtgttcgtc taatgttagt gaaaatgata gagatttttg tagtttggtt
     7801 ggtcgtttta tgattattaa tggtatagat aagttggtta ttaagattaa tagaaaattt
     7861 aataggaaat ctttaagttt agattttagt gttgatttat tcccttctat caaagtttct
     7921 gaattagttt tttttgatga gtttaacaaa acgtgtggtt tttatttttc ttttaattct
     7981 tttacaattt ttaaggcttt tagagatgtt cataatcata ataaaatatc attttatttt
     8041 gcataatttc gggtctgggc cgcagaccag gcccagtgct aacaatatta atttttaatg
     8101 ttaggaattg tttaattctt aattgtgttt ttaaaggtag aataattacc cattcgccct
     8161 ttagccaaca aaaattaagg aggtataaac atggataaaa tggatttgat tcttcaagat
     8221 gaaagactgg gtgagatatt taaagatata gatttaacag ataatgaaaa gagatatctt
     8281 aaatggttat ggaaatggga ttatgaaaca cgtgatactt ttgtatcaat ttttttgaag
     8341 ctaaaaaatg gtggaaaatg atttttttct tatcttgata tattagaaaa aagcgtactc
     8401 acgaagtaag aatttgtaaa aaaagaaggg gggatttttt tggatgagag tttgtacaag
     8461 cagattttaa gtaatattat tattactcgt gattattgta aaaatgtttt agataatata
     8521 aagttcaatg aaaaaataat tgattattat gttatgttac aaaatgatgt ttttattgat
     8581 tttactaata aaataaattc aataagggat tgtaataaat attggtattt ggatgtttat
     8641 aaaaagcaga aaataaagga ttttaaaaag actaatttgt gtaaagataa gttctgtaat
     8701 aattgtaaga aagttaaaca ggcttcaaga atgcaaaaat atattcctga attacagaaa
     8761 tacaaagatg gcttatatca ttttatattt actgttgaaa atgtgccagg tagtgaatta
     8821 agagatacta ttgataggtt gtttaagtct tttaagtcat ttacaaggta tttaagtggt
     8881 aatcttaaaa taaaaggtgt taattttgat aaatggggtt ataaaggctg tgtaaggtct
     8941 ttagaggtaa cttatagtat gattgataat catattatgt atcatccaca cttgcatgtt
     9001 gcgatgatat tagatccttt ttacgatggt tttaatgttg aaaggatgca tataattaat
     9061 aagtttagtt atagctatgg tgttttaaaa aggttgttta ctgatgatga attattaatt
     9121 caaaaaattt ggtatttatt gtttaataat attgaggtta acatggccaa tataaataat
     9181 ttagaggatg gttattcttg tttagttaat aagtttagtg attatgatta tgcggagctg
     9241 tttaagtata tttgtaaaaa tactgatgaa caaggtttac ttatgactta tgatattttt
     9301 aaagatttat attttgcatt acataatgtt catcagatac aaggctatgg ttgtttatat
     9361 aatataagag atgatactca attagattta aaggttgatg acatttataa tgatttgatt
     9421 gatttattac aagttacaga aaatcctata cagtctatgg aaactgtaca ggatttatta
     9481 aaggatactg aatatacaat aataagccgt aagcgtatat ttaagtatct aacacaatta
     9541 tatcataagg attgatattt ataccgtctg tcggactcat gcggaggggg acttgagggg
     9601 gtctcccctc gcattgtacg acagacggta ttattattat acaaattttt tttatgtaat
     9661 tttttttgtg taattttttt atacaaataa tatttcaatt cgagctcggt acccggggat
     9721 cctctagagt cgacctgcag gcatgcacga tacaaattcc tcgtaggcgc tcgggacccc
     9781 tatctagcga acttttagaa aagatataaa acatcagagt atggacagtt gcggatgtac
     9841 ttcagaaaag attagatgtc taaaaagctt gtagttaaag ctttttagac atctaaatct
     9901 aggtgctaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
     9961 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
    10021 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
    10081 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
    10141 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
    10201 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
    10261 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
    10321 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
    10381 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
    10441 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
    10501 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
    10561 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
    10621 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
    10681 gatattctca ttttagccat ttattatttc cttccccttt tctacagtat ttaaagatac
    10741 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
    10801 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
    10861 cgacggagcc gattttgaaa ccacaattat gatagaattt acaagctata aggttattgt
    10921 cctgggtttc aagcattagt ccatgcaagt ttttatgctt tgcccattct atagatatat
    10981 tgataagcgc gctgcctatg ccttgccccc tgaaatcctt acatacgg
//
