LOCUS       sequence-260458-        1160 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding tRNA-SupD for serine amber suppression. *Update: 
            Please use the updated version, Plasmid 198598: SupD v2, which is 
            more stable..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-9"; volume: "6:8130. doi"; pages: " 
            10.1038/ncomms9130"
COMMENT     Sequence Label: SupD
FEATURES             Location/Qualifiers
     source          1..1160
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(851..870)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     gap             1148..1159
                     /estimated_length=12
ORIGIN
        1 cnnnnnnnac ngctttttcn nntatggtat tgataatcct gatatgagta aattgcagtt
       61 tcatttgatg ctcgatgagt ttttctaaga attaattcat gagcggatac atatttgaat
      121 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccgc
      181 ggccgcgctt ctttgagcga acgatcaaaa ataagtggcg ccccatcaaa aaaatattct
      241 caacataaaa aactttgtgt aatacttgta acgctgaatt cggagagatg ccggagcggc
      301 tgaacggacc ggtctctaaa accggagtag gggcaactct accgggggtt caaatccccc
      361 tctctccgcc aggatcctct agagtcgacc tgcagatcct tagcgaaagc taaggatttt
      421 ttttgccgcg gcgcttcttt gagcgaacga tcaaaaataa gtggcgcccc atcaaaaaaa
      481 tattctcaac ataaaaaact ttgtgtaata cttgtaacgc tgaattcgga gagatgccgg
      541 agcggctgaa cggaccggtc tctaaaaccg gagtaggggc aactctaccg ggggttcaaa
      601 tccccctctc tccgccagga tcctctagag tcgacctgca gatccttagc gaaagctaag
      661 gatttttttt gcggccgctt tcaccgtcat caccgaaacg cgcgaggcag cagatcaatt
      721 cgcgcgcgaa ngcgaagcgg catgcattta cgttgacacc atcgaatggt gcaaaacctt
      781 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc nggtggtgaa tgtgaaacca
      841 gtaacgttat acgatgtcgc agagtatgcc cggtgtctct tntcagaccg tttcccgcgt
      901 ggtgaaccag gccagcacgt ttctgcnaaa cgcgggaaaa gtngaagcgn natggnnanc
      961 tgaatncntt cccannncgn gnnnacnnct ggcgggcaac agtcgtgctg atngnnttgc
     1021 nnccnncnnn cnnncctgnn cnnnnnncnn naatngnnnn gnnannnnnt ccnnncnatc
     1081 acnnggnnnc nnnnngngnn nnnngnnnnc nnnnnnnncn annnnnnaan nngngggnnn
     1141 nannnntnnn nnnnnnnnng
//
