LOCUS       sequence-260459-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding tRNA-SupD for serine amber suppression. *Update: 
            Please use the updated version, Plasmid 198598: SupD v2, which is 
            more stable..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-9"; volume: "6:8130. doi"; pages: " 
            10.1038/ncomms9130"
COMMENT     Sequence Label: SupD
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        747..824
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(844..863)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ggnntttttc nnnntatggt attgataatc ctgatatgag taaattgcag tttcatttga
       61 tgctcgatga gtttttctaa gaattaattc atgagcggat acatatttga atgtatttag
      121 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc gcggccgcgc
      181 ttctttgagc gaacgatcaa aaataagtgg cgccccatca aaaaaatatt ctcaacataa
      241 aaaactttgt gtaatacttg taacgctgaa ttcggagaga tgccggagcg gctgaacgga
      301 ccggtctcta aaaccggagt aggggcaact ctaccggggg ttcaaatccc cctctctccg
      361 ccaggatcct ctagagtcga cctgcagatc cttagcgaaa gctaaggatt ttttttgccg
      421 cggcgcttct ttgagcgaac gatcaaaaat aagtggcgcc ccatcaaaaa aatattctca
      481 acataaaaaa ctttgtgtaa tacttgtaac gctgaattcg gagagatgcc ggagcggctg
      541 aacggaccgg tctctaaaac cggagtaggg gcaactctac cgggggttca aatccccctc
      601 tctccgccag gatcctctag agtcgacctg cagatcctta gcgaaagcta aggatttttt
      661 ttgcggccgc tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa ttcgcgcgcg
      721 aaggcgaagc ggcatgcatt tacgttgaca ccatcgaatg gtgcaaaacc tttcgcggta
      781 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt
      841 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggngaacc
      901 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcgganctga
      961 attacattcc caacngcgtg gcacaacact ggngggcaaa cagtcgtnct gattgnntgc
     1021 nncctccagt ctggccctgc acgcgccgtc gcaaatngnc gc
//
