LOCUS       sequence-260460-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding tRNA-SupD for serine amber suppression. *Update: 
            Please use the updated version, Plasmid 198598: SupD v2, which is 
            more stable..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-9"; volume: "6:8130. doi"; pages: " 
            10.1038/ncomms9130"
COMMENT     Sequence Label: SupD
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        753..830
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(850..869)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cnnnnnnnnn ctttttcnnn natggtattg ataatcctga tatgagtaaa ttgcagtttc
       61 atttgatgct cgatgagttt ttctaagaat taattcatga gcggatacat atttgaatgt
      121 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccaccgcgg
      181 ccgcgcttct ttgagcgaac gatcaaaaat aagtggcgcc ccatcaaaaa aatattctca
      241 acataaaaaa ctttgtgtaa tacttgtaac gctgaattcg gagagatgcc ggagcggctg
      301 aacggaccgg tctctaaaac cggagtaggg gcaactctac cgggggttca aatccccctc
      361 tctccgccag gatcctctag agtcgacctg cagatcctta gcgaaagcta aggatttttt
      421 ttgccgcggc gcttctttga gcgaacgatc aaaaataagt ggcgccccat caaaaaaata
      481 ttctcaacat aaaaaacttt gtgtaatact tgtaacgctg aattcggaga gatgccggag
      541 cggctgaacg gaccggtctc taaaaccgga gtaggggcaa ctctaccggg ggttcaaatc
      601 cccctctctc cgccaggatc ctctagagtc gacctgcaga tccttagcga aagctaagga
      661 ttttttttgc ggccgctttc accgtcatca ccgaaacgcg cgaggcagca gatcaattcg
      721 cgcgcgaagg cgaagcggca tgcatttacg ttgacaccat cgaatggtgc aaaacctttc
      781 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
      841 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
      901 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
      961 agctgaatta cattcccaac cgcgtggcac acantggcgg gcaacagtcn tnctgatgnn
     1021 tgccacctcc agtctggccc tgcacgcgcc gncncaaatn nncgc
//
