LOCUS       sequence-26047-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Adams PD, Sellers WR, Sharma SK, Wu AD, Nalin CM, Kaelin WG
  TITLE     Identification of a cyclin-cdk2 recognition motif present in 
            substrates and p21-like cyclin-dependent kinase inhibitors.
  JOURNAL   Mol Cell Biol. 1996 Dec . 16(12):6623-33.
  PUBMED    8943316
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1996 Dec . 16(12):6623-33."
COMMENT     Sequence Label: p21-pRc/CMV
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             115..609
                     /codon_start=1
                     /gene="Homo sapiens CDKN1A"
                     /product="human cyclin-dependent kinase inhibitor 1A"
                     /label=p21
                     /note="mediates p53-dependent arrest in the G1 phase of the
                     cell cycle"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEA
                     RERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQG
                     TAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRK
                     P"
     primer_bind     complement(728..745)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gcngagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttgatatc gaattcgcac tcagaggagg cgccatgtca
      121 gaaccggctg gggatgtccg tcagaaccca tgcggcagca aggcctgccg ccgcctcttc
      181 ggcccagtgg acagcgagca gctgagccgc gactgtgatg cgctaatggc gggctgcatc
      241 caggaggccc gtgagcgatg gaacttcgac tttgtcaccg agacaccact ggagggtgac
      301 ttcgcctggg agcgtgtgcg gggccttggc ctgcccaagc tctaccttcc cacggggccc
      361 cggcgaggcc gggatgagtt gggaggaggc aggcggcctg gcacctcacc tgctctgctg
      421 caggggacag cagaggaaga ccatgtggac ctgtcactgt cttgtaccct tgtgcctcgc
      481 tcaggggagc aggctgaagg gtccccaggt ggacctggag actctcaggg tcgaaaacgg
      541 cggcagacca gcatgacaga tttctaccac tccaaacgcc ggctgatctt ctccaagagg
      601 aagccctaat ccgcccacan nnnnnnnggc agtcctggnn nggggggccc ggtacccccg
      661 ggagannnnn nagttctaga gggccctatt ctatagtgtc ncctaaatgc tagagctcgc
      721 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgnn
      781 ncct
//
