LOCUS       sequence-260486-        8775 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses c-Myc in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Jing H, Hu J, He B, Negron Abril YL, Stupinski J, Weiser K, 
            Carbonaro M, Chiang YL, Southard T, Giannakakou P, Weiss RS, Lin H
  TITLE     A SIRT2-Selective Inhibitor Promotes c-Myc Oncoprotein Degradation 
            and Exhibits Broad Anticancer Activity.
  JOURNAL   Cancer Cell. 2016 Mar 14;29(3):297-310. doi: 
            10.1016/j.ccell.2016.02.007.
  PUBMED    26977881
REFERENCE   2  (bases 1 to 8775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.02"; journalName: "Cancer Cell"; date: 
            "2016-03-14- 14"; volume: "29"; issue: "3"; pages: "297-310"
COMMENT     Sequence Label: pCDH-Flag-c-Myc
FEATURES             Location/Qualifiers
     source          1..8775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    65..181
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        271..474
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     425..445
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     471..495
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             560..583
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             587..1906
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     CDS             1913..1936
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        1987..2198
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2211..2479
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2506..3105
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2506..2525)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3002..3022
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3112..3700
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3165..3185)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3583..3594)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3774..4007
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4079..4200
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4116..4135)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4170..4189
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4240..4375
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4408..4424)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4408..4424)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4421..4443)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4432..4448
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4456..4462,4463..4480,4481..4486))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4501..4522
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4639..4656)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4810..5398)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(5569..6360,6361..6429))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6192..6211
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6430..6534)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6602..6620
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6658..6680)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6780..6799
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6993..7015
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7007..7024
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7008..7024
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7039..7265
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7266..7446
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7493..7618
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8111..8344
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8529..8573
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt
       61 taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
      121 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt
      181 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      241 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      301 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      361 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      421 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
      481 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
      541 tagagctagc gaattcatgg attacaagga tgacgacgat aagggaatgc ccctcaacgt
      601 tagcttcacc aacaggaact atgacctcga ctacgactcg gtgcagccgt atttctactg
      661 cgacgaggag gagaacttct accagcagca gcagcagagc gagctgcagc ccccggcgcc
      721 cagcgaggat atctggaaga aattcgagct gctgcccacc ccgcccctgt cccctagccg
      781 ccgctccggg ctctgctcgc cctcctacgt tgcggtcaca cccttctccc ttcggggaga
      841 caacgacggc ggtggcggga gcttctccac ggccgaccag ctggagatgg tgaccgagct
      901 gctgggagga gacatggtga accagagttt catctgcgac ccggacgacg agaccttcat
      961 caaaaacatc atcatccagg actgtatgtg gagcggcttc tcggccgccg ccaagctcgt
     1021 ctcagagaag ctggcctcct accaggctgc gcgcaaagac agcggcagcc cgaaccccgc
     1081 ccgcggccac agcgtctgct ccacctccag cttgtacctg caggatctga gcgccgccgc
     1141 ctcagagtgc atcgacccct cggtggtctt cccctaccct ctcaacgaca gcagctcgcc
     1201 caagtcctgc gcctcgcaag actccagcgc cttctctccg tcctcggatt ctctgctctc
     1261 ctcgacggag tcctccccgc agggcagccc cgagcccctg gtgctccatg aggagacacc
     1321 gcccaccacc agcagcgact ctgaggagga acaagaagat gaggaagaaa tcgatgttgt
     1381 ttctgtggaa aagaggcagg ctcctggcaa aaggtcagag tctggatcac cttctgctgg
     1441 aggccacagc aaacctcctc acagcccact ggtcctcaag aggtgccacg tctccacaca
     1501 tcagcacaac tacgcagcgc ctccctccac tcggaaggac tatcctgctg ccaagagggt
     1561 caagttggac agtgtcagag tcctgagaca gatcagcaac aaccgaaaat gcaccagccc
     1621 caggtcctcg gacaccgagg agaatgtcaa gaggcgaaca cacaacgtct tggagcgcca
     1681 gaggaggaac gagctaaaac ggagcttttt tgccctgcgt gaccagatcc cggagttgga
     1741 aaacaatgaa aaggccccca aggtagttat ccttaaaaaa gccacagcat acatcctgtc
     1801 cgtccaagca gaggagcaaa agctcatttc tgaagaggac ttgttgcgga aacgacgaga
     1861 acagttgaaa cacaaacttg aacagctacg gaactcttgt gcgtaactcg aggattacaa
     1921 ggatgacgac gataagtagg gatccgcggc cgcgaaggat ctgcgatcgc tccggtgccc
     1981 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     2041 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     2101 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     2161 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
     2221 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
     2281 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
     2341 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
     2401 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
     2461 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagatgac cgagtacaag
     2521 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     2581 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     2641 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     2701 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     2761 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     2821 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     2881 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     2941 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     3001 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     3061 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagtcga caatcaacct
     3121 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3181 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3241 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     3301 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     3361 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     3421 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     3481 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     3541 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     3601 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     3661 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
     3721 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     3781 gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt
     3841 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     3901 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     3961 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     4021 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     4081 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4141 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4201 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     4261 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     4321 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
     4381 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     4441 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     4501 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     4561 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     4621 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4681 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4741 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4801 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4861 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4921 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4981 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     5041 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5101 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5161 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5221 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     5281 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5341 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5401 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5461 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5521 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5581 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5641 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5701 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5761 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5821 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5881 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5941 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6001 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6061 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6121 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6181 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6241 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6301 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6361 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6421 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6481 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6541 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     6601 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     6661 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     6721 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     6781 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     6841 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     6901 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     6961 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     7021 cagtgccaag ctgacgcgtg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     7081 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     7141 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     7201 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     7261 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7321 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7381 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7441 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     7501 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     7561 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     7621 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     7681 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     7741 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     7801 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     7861 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     7921 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc actgatcttc agacctggag
     7981 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     8041 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     8101 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     8161 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     8221 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     8281 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     8341 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     8401 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     8461 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     8521 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     8581 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     8641 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     8701 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     8761 gaatagaaga agaag
//
