LOCUS       sequence-260600-        3066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes an iconic representation of an apple.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Banal JL, Shepherd TR, Berleant J, Huang H, Reyes M, Ackerman CM, 
            Blainey PC, Bathe M
  TITLE     Random access DNA memory using Boolean search in an archival file 
            storage system.
  JOURNAL   Nat Mater. 2021 Sep;20(9):1272-1280. doi: 
            10.1038/s41563-021-01021-3. Epub 2021 Jun 10.
  PUBMED    34112975
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41563-021-01021-3"; journalName: "Nat Mater"; date: 
            "2021-09"; volume: "20"; issue: "9"; pages: "1272-1280"
COMMENT     Sequence Label: pUCIDT-Apple1
FEATURES             Location/Qualifiers
     source          1..3066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(35..57)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     157..176
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     370..392
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     384..401
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     385..401
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(851..867)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(851..867)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(864..886)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    875..891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(899..905,906..923,924..929))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    944..965
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1082..1099)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1253..1841)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1333..1352)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2012..2803,2804..2872))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2635..2654
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2873..2977)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3045..3063
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
       61 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
      121 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
      181 gagtgcacca aatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
      241 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctcat
      301 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
      361 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgcaacgcga tgacgatgga
      421 tagcgattca tcgatgagct gacccgatcg ccgccgccgg agggttgcgt ttgagacggg
      481 cgacagatga attcgtcgtc gtcccctcaa actatcatat ctcgtcgatc gctcagaatg
      541 agctgtgacg cgtgatctaa catgattttt ggaacgggat cgggaacggt ggtcaacctg
      601 cttttctctt cggcttagca tgcagtctca ttccgacgat catcgcgctc ccataggata
      661 taccaaattc atatgaacag ttcctgtcta taggtccata attcagttct tacatgctga
      721 gaattgaatg aggctttcac ttttctgcgg atatcgctga cactatagac gtgctcgctg
      781 aaaaggtggc atcaatctgc agatcagttc tggaccagcg agctgtgctg cgactcgtgg
      841 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      901 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      961 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     1021 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1081 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1141 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1201 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1261 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1321 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1381 ttccgaccct gtcgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1441 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1501 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1561 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1621 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1681 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1741 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1801 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1861 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1921 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1981 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2041 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2101 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2161 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2221 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2281 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2341 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2401 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2461 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2521 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2581 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     2641 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2701 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     2761 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     2821 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactctacc
     2881 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     2941 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3001 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3061 ggccct
//
