LOCUS       sequence-260601-        3401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes an iconic representation of a house cat.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Banal JL, Shepherd TR, Berleant J, Huang H, Reyes M, Ackerman CM, 
            Blainey PC, Bathe M
  TITLE     Random access DNA memory using Boolean search in an archival file 
            storage system.
  JOURNAL   Nat Mater. 2021 Sep;20(9):1272-1280. doi: 
            10.1038/s41563-021-01021-3. Epub 2021 Jun 10.
  PUBMED    34112975
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41563-021-01021-3"; journalName: "Nat Mater"; date: 
            "2021-09"; volume: "20"; issue: "9"; pages: "1272-1280"
COMMENT     Sequence Label: pUCIDT-Cat1
FEATURES             Location/Qualifiers
     source          1..3401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(34..56)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     156..175
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     369..391
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     383..400
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     384..400
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1185..1201)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1185..1201)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1198..1220)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1209..1225
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1233..1239,1240..1257,1258..1263))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1278..1299
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1416..1433)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1587..2175)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1667..1686)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2346..3137,3138..3206))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2969..2988
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3207..3311)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3379..3397
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
       61 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
      121 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
      181 agtgcaccaa atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
      241 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcatc
      301 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
      361 agggttttcc cagtcacgac gttgtaaaac gacggccagt gcaacgcgat gacgatggat
      421 agcgattcat cgatgagctg acccgatcgc cgccgccgga gggttgcgtt tgagacgggc
      481 gacagatgaa ttcgtcgtcg tcccctcaaa ctatcatgtc tatgagtaca ctcgcgatgt
      541 gacagtcaca gatcgtcgca taagtgctcc caccgtgtca ttggcgccag taatgtgcca
      601 tgtcattcac tctaagaata agtgaaaata tgaagctggg agcgcccgga gattagtctg
      661 ccacgactca acctctagat cgaacgtcct ctctccgata gttaggccat actggtacgt
      721 catcaaggct tcgggtttga atgagcacca cagtcctcgc ctttaactgc gtccggcctc
      781 ttgcaggatt atctttagtt atactggaag taggtactga aaagcatctg cgccctagca
      841 aggcttattt ttaggatctc agtgggatgg aaaagcgtat gccacatggt agcgaaaaag
      901 tcgtcttgct gtgccggagt cggctacggc ctgatgagct gaggcggagg gcttgccttc
      961 gaaagctcct actaacaatt caataaatgt gggaatgcta cataaattgt cgtagtaccg
     1021 tcagagatag gagacgggtc gattaacact tctacgcagg gttataatag tttgtgacac
     1081 tcagcaatcg cgacactaca cgctacgcac gctgaaaagg tggcatcaat ctgcagatca
     1141 gttctggacc agcgagctgt gctgcgactc gtggcgtaat catggtcata gctgtttcct
     1201 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     1261 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     1321 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     1381 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1441 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1501 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1561 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1621 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1681 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgtcgct taccggatac
     1741 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1801 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1861 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1921 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1981 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2041 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2101 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2161 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2221 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2281 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2341 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2401 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2461 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     2521 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2581 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     2641 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     2701 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2761 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     2821 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     2881 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     2941 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3001 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3061 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3121 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3181 gcgacacgga aatgttgaat actcatactc tacctttttc aatattattg aagcatttat
     3241 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3301 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     3361 atgacattaa cctataaaaa taggcgtatc acgaggccct t
//
