LOCUS       sequence-260633-       11653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of Cas9-HF1RA in 
            mammalian cells (codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11653)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 11653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-HF1RA-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..11653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1719..1836
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1905..2116
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2136..2145
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2145..2168
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2175..2195
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2220..6320
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGALSRKLINGIRDKQSGKTILDFLKSDGFANRNFMA
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRAITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6321..6368
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     promoter        6383..6870
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6418..6441)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6803..6822
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6891..7490
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6891..6910)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7387..7407
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7506..8094
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7559..7579)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7977..7988)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8166..8399
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8477..8598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8514..8533)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8568..8587
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8638..8773
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8793..8812)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8794..8812)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8822..8839)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8822..8838)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8831..8853)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8980..9435
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9067..9086)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9277..9298
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9461..9565
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9566..9634,9635..10426)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9784..9803)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10597..11185
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11086..11105
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11339..11356
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11473..11494
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11509..11514,11515..11532,11533..11539)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11547..11563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11552..11574
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11571..11587
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11571..11587
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11606..11626
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11608..11626
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca gaccacaatt ttaaaagaaa aggggggatt
     1741 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     1801 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     1861 gatccacttt ggcgccggct cgagtggctc cggtgcccgt cagtgggcag agcgcacatc
     1921 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
     1981 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     2041 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     2101 tgccgccaga acacaggtgt cgtgacgcgg gatccgccac catggattac aaagacgatg
     2161 acgataagat ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg
     2221 acaagaagta cagcatcggc ctggacatcg gcaccaactc tgtgggctgg gccgtgatca
     2281 ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca
     2341 gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca
     2401 cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc
     2461 tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg
     2521 aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca
     2581 tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac
     2641 tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga
     2701 tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg
     2761 acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca
     2821 acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc
     2881 tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggaaacctga
     2941 ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg
     3001 ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga
     3061 tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc
     3121 tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga
     3181 tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc
     3241 agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct
     3301 acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa
     3361 agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc
     3421 agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca
     3481 ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga
     3541 agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat
     3601 tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg
     3661 tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc
     3721 tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata
     3781 acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg
     3841 gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga
     3901 agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg
     3961 gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca
     4021 aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga
     4081 ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc
     4141 tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc tggggcgccc
     4201 tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt
     4261 tcctgaagtc cgacggcttc gccaacagaa acttcatggc cctgatccac gacgacagcc
     4321 tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg
     4381 agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga
     4441 aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg
     4501 aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga
     4561 agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg
     4621 aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata
     4681 tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gaccatatcg
     4741 tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc agaagcgaca
     4801 agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact
     4861 actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca
     4921 aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg
     4981 tggaaacccg ggccatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta
     5041 agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc
     5101 tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc
     5161 accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc
     5221 ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga
     5281 tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca
     5341 tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc
     5401 tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca
     5461 ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga
     5521 caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca
     5581 gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt
     5641 ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag
     5701 agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc
     5761 tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact
     5821 ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga
     5881 agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact
     5941 atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc
     6001 acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc
     6061 tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca
     6121 tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg
     6181 ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg
     6241 tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc
     6301 tgtctcagct gggaggcgac aagcgtcctg ctgctactaa gaaagctggt caagctaaga
     6361 aaaagaaata agctagccta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     6421 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     6481 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     6541 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     6601 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     6661 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     6721 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg ggcgggcgcc
     6781 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     6841 ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt
     6901 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     6961 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     7021 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     7081 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     7141 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     7201 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     7261 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     7321 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     7381 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     7441 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga ttctagaaag
     7501 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     7561 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     7621 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     7681 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7741 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7801 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7861 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     7921 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7981 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     8041 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac
     8101 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8161 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     8221 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8281 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8341 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8401 tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     8461 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     8521 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8581 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     8641 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8701 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8761 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     8821 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     8881 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     8941 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     9001 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     9061 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     9121 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     9181 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     9241 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     9301 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     9361 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     9421 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     9481 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     9541 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     9601 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     9661 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     9721 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     9781 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     9841 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     9901 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     9961 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
    10021 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
    10081 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
    10141 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
    10201 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
    10261 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
    10321 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    10381 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    10441 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    10501 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    10561 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    10621 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    10681 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10741 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10801 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    10861 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    10921 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    10981 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    11041 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    11101 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    11161 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    11221 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    11281 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    11341 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
    11401 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
    11461 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
    11521 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
    11581 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
    11641 gctgcaagct taa
//
