LOCUS       sequence-260645-        8595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full length SIRT2 isoform 1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8595)
  AUTHORS   Jing H, Hu J, He B, Negron Abril YL, Stupinski J, Weiser K, 
            Carbonaro M, Chiang YL, Southard T, Giannakakou P, Weiss RS, Lin H
  TITLE     A SIRT2-Selective Inhibitor Promotes c-Myc Oncoprotein Degradation 
            and Exhibits Broad Anticancer Activity.
  JOURNAL   Cancer Cell. 2016 Mar 14;29(3):297-310. doi: 
            10.1016/j.ccell.2016.02.007.
  PUBMED    26977881
REFERENCE   2  (bases 1 to 8595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.02"; journalName: "Cancer Cell"; date: 
            "2016-03-14- 14"; volume: "29"; issue: "3"; pages: "297-310"
COMMENT     Sequence Label: pCDH-SIRT2-Flag
FEATURES             Location/Qualifiers
     source          1..8595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    263..384
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(300..319)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     354..373
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      424..559
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(592..608)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(592..608)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(605..627)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    616..632
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(640..646,647..664,665..670))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    685..706
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(823..840)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(994..1582)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(1753..2544,2545..2613))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2376..2395
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2614..2718)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2786..2804
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2842..2864)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2964..2983
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3177..3199
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3191..3208
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3192..3208
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3223..3449
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3450..3630
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3677..3802
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4295..4528
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4713..4757
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5024..5140
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5230..5433
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5384..5404
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5430..5454
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6692..6715
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        6766..6977
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6990..7258
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             7285..7884
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7285..7304)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7781..7801
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7891..8479
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7944..7964)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8362..8373)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             join(8553..8595,1..191)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
       61 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      121 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      181 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
      241 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
      301 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      361 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
      421 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
      481 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
      541 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct
      601 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      661 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      721 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
      781 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
      841 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      901 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      961 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1021 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1081 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1141 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1201 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1261 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1321 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1381 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1441 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1501 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1561 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1621 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1681 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1741 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1801 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     1861 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1921 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1981 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2041 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2101 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2161 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2221 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2281 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2341 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     2401 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2461 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2521 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2581 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2641 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2701 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     2761 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     2821 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     2881 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     2941 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     3001 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
     3061 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     3121 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     3181 gtcacgacgt tgtaaaacga cggccagtgc caagctgacg cgtgtagtct tatgcaatac
     3241 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     3301 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     3361 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     3421 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     3481 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     3541 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     3601 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     3661 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     3721 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     3781 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     3841 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     3901 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     3961 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     4021 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     4081 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     4141 ggccactgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     4201 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     4261 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     4321 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     4381 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     4441 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     4501 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     4561 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     4621 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     4681 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     4741 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     4801 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     4861 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     4921 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     4981 gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg
     5041 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     5101 ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgatacta gtattatgcc
     5161 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     5221 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     5281 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     5341 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     5401 cgtgtacggt gggaggttta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     5461 agacgccatc cacgctgttt tgacctccat agaagattct agagctagcg aattcatggc
     5521 agagccagac ccctctcacc ctctggagac ccaggcaggg aaggtgcagg aggctcagga
     5581 ctcagattca gactctgagg gaggagccgc tggtggagaa gcagacatgg acttcctgcg
     5641 gaacttattc tcccagacgc tcagcctggg cagccagaag gagcgtctgc tggacgagct
     5701 gaccttggaa ggggtggccc ggtacatgca gagcgaacgc tgtcgcagag tcatctgttt
     5761 ggtgggagct ggaatctcca catccgcagg catccccgac tttcgctctc catccaccgg
     5821 cctctatgac aacctagaga agtaccatct tccctaccca gaggccatct ttgagatcag
     5881 ctatttcaag aaacatccgg aacccttctt cgccctcgcc aaggaactct atcctgggca
     5941 gttcaagcca accatctgtc actacttcat gcgcctgctg aaggacaagg ggctactcct
     6001 gcgctgctac acgcagaaca tagataccct ggagcgaata gccgggctgg aacaggagga
     6061 cttggtggag gcgcacggca ccttctacac atcacactgc gtcagcgcca gctgccggca
     6121 cgaatacccg ctaagctgga tgaaagagaa gatcttctct gaggtgacgc ccaagtgtga
     6181 agactgtcag agcctggtga agcctgatat cgtctttttt ggtgagagcc tcccagcgcg
     6241 tttcttctcc tgtatgcagt cagacttcct gaaggtggac ctcctcctgg tcatgggtac
     6301 ctccttgcag gtgcagccct ttgcctccct catcagcaag gcacccctct ccacccctcg
     6361 cctgctcatc aacaaggaga aagctggcca gtcggaccct ttcctgggga tgattatggg
     6421 cctcggagga ggcatggact ttgactccaa gaaggcctac agggacgtgg cctggctggg
     6481 tgaatgcgac cagggctgcc tggcccttgc tgagctcctt ggatggaaga aggagctgga
     6541 ggaccttgtc cggagggagc acgccagcat agatgcccag tcgggggcgg gggtccccaa
     6601 ccccagcact tcagcttccc ccaagaagtc cccgccacct gccaaggacg aggccaggac
     6661 aacagagagg gagaaacccc agggactcga ggattacaag gatgacgacg ataagtaggg
     6721 atccgcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat
     6781 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa
     6841 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     6901 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     6961 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc
     7021 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg
     7081 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc
     7141 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga
     7201 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca
     7261 agctgtgacc ggcgcctacg ctagatgacc gagtacaagc ccacggtgcg cctcgccacc
     7321 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     7381 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     7441 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     7501 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     7561 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     7621 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     7681 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     7741 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     7801 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     7861 atgacccgca agcccggtgc ctgagtcgac aatcaacctc tggattacaa aatttgtgaa
     7921 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7981 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     8041 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     8101 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     8161 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     8221 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     8281 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     8341 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     8401 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     8461 ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga
     8521 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     8581 ataagatctg ctttt
//
