LOCUS       sequence-26071-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Czlapinski JL, Schelle MW, Miller LW, Laughlin ST, Kohler JJ, 
            Cornish VW, Bertozzi CR
  TITLE     Conditional glycosylation in eukaryotic cells using a biocompatible 
            chemical inducer of dimerization.
  JOURNAL   J Am Chem Soc. 2008 Oct 8. 130(40):13186-7.
  PUBMED    18788807
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2008 Oct 8. 130(40):13186-7."
COMMENT     Sequence Label: Vh-HA-DHFR
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(88..561)
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             complement(562..588)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctctagactc gagcggccgc cactgtgctg gatatctgca gaattccacc acactggact
       61 agtggatccg agctcggtac ctctagtccg ccgctccaga atctcaaagc aatagctgtg
      121 agagttctgc gcatcagcat cgtggaattc gctgaatacc gattcccagt catccggctc
      181 gtaatccggg aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca gatacagttt
      241 ttgcgctttt ggcaagaact gttcataaac gcgaccgccg ccaatcacca tgatttctgg
      301 tacgtcacca cacgccgcga tggcttcatc caccgacttc acccacgtta cgcgatcgtc
      361 cgtacccggt tgactgctga ggataatatt tttgcgtcct ggcaacggac gaccgattga
      421 ttcccaggta tggcggccca taatcacggg tttatttaag gtgttgcgtt taaaccaggc
      481 gagatcggca ggcaggttcc acggcatggc gttttccatg ccgataacgc gatctaccgc
      541 taacgccgca atcagactga ttgcgtagtc tggtacgtcg tacggatatc tagactcgag
      601 gtcgacctgc agttggacct gggagtgcgc gcctgtggag agaaaggcaa agtggatgtc
      661 attgtcaccc atatatatgt ccagacctca agcctgctac tgttaacccc ttacctgtag
      721 ctnnnnntac aaanaaanag ganngataca acctnccntc ccatgggtgg cccacnncnn
      781 nntnantttt ccacgttgcc nagtaagc
//
