LOCUS       sequence-260713-        6719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 553, Emission: 576.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6719)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTurboRFP-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..6719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        134..437
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        438..641
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     592..612
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      693..702
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      697..706
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(699..761,762..1919)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     CDS             1980..2672
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     CDS             2742..3434
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     polyA_signal    3580..3701
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3617..3636)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3671..3690
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3708..4163)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3845..3866)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4057..4076
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4190..4294
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4296..4653
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4296..4487
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4319..4339)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4504..4639
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4688..5482
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4688..5482
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4742..4761)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5352..5371
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5670..5689)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5714..5761
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6090..6678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6579..6598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
       61 taccgccatg cattagttat taatagtaat caattacggg gtcattagtt catagcccat
      121 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      181 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      241 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      301 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      361 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      421 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      481 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      541 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      601 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
      661 tccgctagcg ctaccggact cagatctcga gcgccaccat ggcggccccg ggcgcccggc
      721 gtccgctgct cctgttgctg ctggcaggcc ttgcacacag cgccccagca ctgttcgagg
      781 tgaaagacaa caacggcaca gcgtgtataa tggccagctt ctctgcctcc tttctgacca
      841 cctatgaggc tggacatgtt tctaaggtct cgaatatgac cctgccagcc tctgcagaag
      901 tcctgaagaa tagcagctct tgtggtgaaa agaatgcttc tgagcccacc ctcgcaatca
      961 cctttggaga aggatattta ctgaaactca ccttcacaaa aaacacaaca cgttacagtg
     1021 tccagcacat gtatttcaca tataacctgt cagacacaca attctttccc aatgccagct
     1081 ccaaagggcc cgacactgtg gattccacaa ctgacatcaa ggcagacatc aacaaaacat
     1141 accgatgtgt cagcgacatc agggtctaca tgaagaatgt gaccattgtg ctctgggacg
     1201 ctactatcca ggcctacctg ccgagtagca acttcagcaa ggaagagaca cgctgcccac
     1261 aggatcaacc ttccccaact actgggccac ccagcccctc accaccactt gtgcccacaa
     1321 accccagtgt gtccaagtac aatgtgactg gtgacaatgg aacctgcctg ctggcctcta
     1381 tggcactgca actcaacatc acctacatga agaaggacaa cacgactgtg accagagcat
     1441 tcaacatcaa cccaagtgac aaatatagtg ggacttgcgg tgcccagttg gtgaccctga
     1501 aggtggggaa caagagcaga gtcctggagc tgcagtttgg gatgaatgcc acttctagcc
     1561 tgtttttcct gcaaggagtt cagttgaaca tgactcttcc tgatgccata gagcccacgt
     1621 tcagcacctc caactattcc ctgaaagctc ttcaggccag tgtcggcaac tcatacaagt
     1681 gcaacagtga ggagcacatc tttgtcagca aggcgctcgc cctcaatgtc ttcagcgtgc
     1741 aagtccaggc tttcagggta gaaagtgaca ggtttgggtc tgtggaagag tgtgtacagg
     1801 acggtaacaa catgctgatc cccattgctg tgggcggggc cctggcaggg ctggtcctca
     1861 tcgtcctcat cgcctacctc atcggcagga agaggagtca cgcgggctat cagaccatct
     1921 cggaattcgg ctccaccggc tccaccggct ccaccggcgc ggatccaccg gtcgccacca
     1981 tgagcgagct gatcaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca
     2041 accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
     2101 tgaagatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca
     2161 gcttcatgta cggcagcaaa gccttcatca accacaccca gggcatcccc gacttcttta
     2221 agcagtcctt ccctgagggc ttcacatggg agagaatcac cacatacgaa gacgggggcg
     2281 tgctgaccgc tacccaggac accagcttcc agaacggctg catcatctac aacgtcaaga
     2341 tcaacggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca cgcggctggg
     2401 aggccaacac cgagatgctg taccccgctg acggcggcct gagaggccac agccagatgg
     2461 ccctgaagct cgtgggcggg ggctacctgc actgctcctt caagaccaca tacagatcca
     2521 agaaacccgc taagaacctc aagatgcccg gcttccactt cgtggaccac agactggaaa
     2581 gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgagatggct gtggccaagt
     2641 actgcgacct ccctagcaaa ctggggcaca gagggcatgg caccggcagc accggcagcg
     2701 gcagctccgg taccgcctcc tccgaggaca acaacatggc catgagcgag ctgatcaagg
     2761 agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca
     2821 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgaagatc aaggtggtcg
     2881 agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca
     2941 aagccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccctgagg
     3001 gcttcacatg ggagagaatc accacatacg aagacggggg cgtgctgacc gctacccagg
     3061 acaccagctt ccagaacggc tgcatcatct acaacgtcaa gatcaacggg gtgaacttcc
     3121 catccaacgg ccctgtgatg cagaagaaaa cacgcggctg ggaggccaac accgagatgc
     3181 tgtaccccgc tgacggcggc ctgagaggcc acagccagat ggccctgaag ctcgtgggcg
     3241 ggggctacct gcactgctcc ttcaagacca catacagatc caagaaaccc gctaagaacc
     3301 tcaagatgcc cggcttccac ttcgtggacc acagactgga aagaatcaag gaggccgaca
     3361 aagagaccta cgtcgagcag cacgagatgg ctgtggccaa gtactgcgac ctccctagca
     3421 aactggggca cagagatgaa tccggactca gatcttgagc ggccgcgact ctagatcata
     3481 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     3541 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     3601 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3661 cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt
     3721 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     3781 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     3841 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     3901 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     3961 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     4021 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     4081 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     4141 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4201 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4261 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     4321 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4381 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4441 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4501 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4561 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     4621 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     4681 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     4741 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     4801 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     4861 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     4921 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     4981 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     5041 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     5101 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     5161 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     5221 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     5281 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     5341 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     5401 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     5461 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     5521 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     5581 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     5641 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     5701 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     5761 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     5821 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     5881 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     5941 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6001 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6061 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6121 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6181 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6241 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6301 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6361 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6421 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6481 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6541 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6601 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6661 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttg
//
