LOCUS       sequence-26072-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Czlapinski JL, Schelle MW, Miller LW, Laughlin ST, Kohler JJ, 
            Cornish VW, Bertozzi CR
  TITLE     Conditional glycosylation in eukaryotic cells using a biocompatible 
            chemical inducer of dimerization.
  JOURNAL   J Am Chem Soc. 2008 Oct 8. 130(40):13186-7.
  PUBMED    18788807
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2008 Oct 8. 130(40):13186-7."
COMMENT     Sequence Label: Vh-HA-2xDHFR
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(95..568)
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
ORIGIN
        1 acnnnnncnc tagactcgag cggccgccac tgtgctggat atctgcagaa ttccaccaca
       61 ctggactagt ggatccgagc tcggtacctc tagtccgccg ctccagaatc tcaaagcaat
      121 agctgtgaga gttctgcgca tcagcatcgt ggaattcgct gaataccgat tcccagtcat
      181 ccggctcgta atccgggaaa tgggtgtcgc cttccacttc tgcgtcgata tgcgtcagat
      241 acagtttttg cgcttttggc aagaactgtt cataaacgcg accgccgcca atcaccatga
      301 tttctggtac gtcaccacac gccgcgatgg cttcatccac cgacttcacc cacgttacgc
      361 gatcgtccgt acccggttga ctgctgagga taatattttt gcgtcctggc aacggacgac
      421 cgattgattc ccaggtatgg cggcccataa tcacgggttt atttaaggtg ttgcgtttaa
      481 accaggcgag atcggcaggc aggttccacg gcatggcgtt ttccatgccg ataacgcgat
      541 ctaccgctaa cgccgcaatc agactgattc tagtccgccg ctccagaatc tcaaagcaat
      601 agctgtgaga gttctgcgca tcagcatcgt ggaattcgct gaataccgat tcccagtcat
      661 ccggctcgta atccgggaaa tgggtgtcgc cttccacttc tgcgtcgata tgcgtcagat
      721 acagtttttg cgcttttggc aagaactgtt cataaacnnn ncgcgcgccc aatcanccat
      781 gnttnncngg tacnnccncc nnncnnnncc ncg
//
