LOCUS       sequence-26074-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Czlapinski JL, Schelle MW, Miller LW, Laughlin ST, Kohler JJ, 
            Cornish VW, Bertozzi CR
  TITLE     Conditional glycosylation in eukaryotic cells using a biocompatible 
            chemical inducer of dimerization.
  JOURNAL   J Am Chem Soc. 2008 Oct 8. 130(40):13186-7.
  PUBMED    18788807
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2008 Oct 8. 130(40):13186-7."
COMMENT     Sequence Label: Vh-HA-FKBP
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(88..408)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             complement(409..435)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     sig_peptide     complement(join(469..480,563..607))
                     /codon_start=1
                     /product="modified signal sequence from the mouse 
                     immunoglobulin heavy chain variable region"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     CDS             complement(join(469..479,562..607))
                     /codon_start=1
                     /product="signal sequence from the mouse immunoglobulin 
                     heavy chain variableregion"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     regulatory      604..613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(670..721)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(699..719)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctctagactc gagcggccgc cactgtgctg gatatctgca gaattccacc acactggact
       61 agtggatccg agctcggtac ctctagtttc cagttttaga agctccacat cgaagacgag
      121 agtggcatgt ggtgggatga tgcctgggtg cccagttgca ccataggcat aatctggaga
      181 tatagtcagt ttggctctct gacccacact catctgggca accccttctt cccagcctcg
      241 gatcacctcc tgcttgccta gcataaactt aaagggcttg tttctgtccc gggaggaatc
      301 aaatttcttt ccatcttcaa gcatcccggt gtagtgcacc acgcaggtct ggccgcgctt
      361 ggggaaggtg cgcccgtctc ctggggagat ggtttccacc tgcactcctg cgtagtctgg
      421 tacgtcgtac ggatatctag actcgaggtc gacctgcagt tggacctggg agtgcgcgcc
      481 tgtggagaga aaggcaaagt ggatgtcatt gtcacccata tatatgtcca gacctcaagc
      541 ctgctactgt taacccctta cctgtagctg ttgctaccaa gaagaggatg atacagctcc
      601 atcccatggt ggccacgtcg tattaatttc cacgtgccag taagcagtgg gttctctagt
      661 tagccagaaa gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
      721 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattgg
//
