LOCUS       sequence-26078-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Czlapinski JL, Schelle MW, Miller LW, Laughlin ST, Kohler JJ, 
            Cornish VW, Bertozzi CR
  TITLE     Conditional glycosylation in eukaryotic cells using a biocompatible 
            chemical inducer of dimerization.
  JOURNAL   J Am Chem Soc. 2008 Oct 8. 130(40):13186-7.
  PUBMED    18788807
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2008 Oct 8. 130(40):13186-7."
COMMENT     Sequence Label: Vh-HA-2xFRB
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(94..372)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             complement(379..657)
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             complement(658..684)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 acgggcctct agactcgagc ggccgccact gtgctggata tctgcagaat tccaccacac
       61 tggactagtg gatccgagct cggtacctct agtctttgag attcgtcgga acacatgata
      121 atagaggtcc caggcttggg tgaggtcctt gacattccct gatttcatgt acttcctgca
      181 ccactcttgg gcctccatta aatctcgacc ataggcctga ttaaaggatg tttccttcag
      241 agtctggggg ccccgttcca tcatagcatg caagggctcc agcacctcaa acatgccttt
      301 cacgttcctt tccccaaagt acaaacgaga tgcctcttcc aggccttcat gccacatctc
      361 atgccagagg attctagtct ttgagattcg tcggaacaca tgataataga ggtcccaggc
      421 ttggaggagg tccttgacat tccctgattt catgtacttc ctgcaccact cttgggcctc
      481 cattaaatct cgaccatagg cctgattaaa ggatgtttcc ttcagagtct gggggccccg
      541 ttccatcata gcatgcaagg gctccagcac ctcaaacatg cctttcacgt tcctttcccc
      601 aaagtacaaa cgagatgcct cttccaggcc ttcatgccac atctcatgcc agaggattgc
      661 gtagtctggt acgtcgtacg gatatctaga ctcgaggtcg acctgcagtt ggacctggga
      721 gtgcgcgcct gtggagagaa aggcaaagtg gatgtcattg tcacccatat atatgtccag
      781 acctcaagcc tgctactgtt aaccccttac ctgtagctgt
//
