LOCUS       sequence-260787-        3117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZA2-pLlacO-oxyR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: fIM1264
FEATURES             Location/Qualifiers
     source          1..3117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..19
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    26..42
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             94..105
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1059..1145
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1182..1199)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1316..1861)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1396..1415)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1973..2067)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2098..2892)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2209..2228)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2819..2838
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3071..3089
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatcagc
       61 aggacgcact gaccttaatt aaaagaattc attaaagagg agaaaggtac catgaatatt
      121 cgtgatcttg agtacctggt ggcattggct gaacaccgcc attttcggcg tgcggcagat
      181 tcctgccacg ttagccagcc gacgcttagc gggcaaattc gtaagctgga agatgagctg
      241 ggcgtgatgt tgctggagcg gaccagccgt aaagtgttgt tcacccaggc gggaatgctg
      301 ctggtggatc aggcgcgtac cgtgctgcgt gaggtgaaag tccttaaaga gatggcaagc
      361 cagcagggcg agacgatgtc cggaccgctg cacattggtt tgattcccac agttggaccg
      421 tacctgctac cgcatattat ccctatgctg caccagacct ttccaaagct ggaaatgtat
      481 ctgcatgaag cacagaccca ccagttactg gcgcaactgg acagcggcaa actcgattgc
      541 gtgatcctcg cgctggtgaa agagagcgaa gcattcattg aagtgccgtt gtttgatgag
      601 ccaatgttgc tggctatcta tgaagatcac ccgtgggcga accgcgaatg cgtaccgatg
      661 gccgatctgg caggggaaaa actgctgatg ctggaagatg gtcactgttt gcgcgatcag
      721 gcaatgggtt tctgttttga agccggggcg gatgaagata cacacttccg cgcgaccagc
      781 ctggaaactc tgcgcaacat ggtggcggca ggtagcggga tcactttact gccagcgctg
      841 gctgtgccgc cggagcgcaa acgcgatggg gttgtttatc tgccgtgcat taagccggaa
      901 ccacgccgca ctattggcct ggtttatcgt cctggctcac cgctgcgcag ccgctatgag
      961 cagctggcag aggccatccg cgcaagaatg gatggccatt tcgataaagt tttaaaacag
     1021 gcggtttaag gatcccatgg tacgcgtgct agaggcatca aataaaacga aaggctcagt
     1081 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     1141 caaatccgcc gccctagacc tagggggata tattccgctt cctcgctcac tgactcgcta
     1201 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
     1261 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
     1321 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
     1381 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
     1441 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
     1501 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
     1561 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1621 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
     1681 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
     1741 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
     1801 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
     1861 agaagatcat cttattaatc agataaaata tttctagatt tcagtgcaat ttatctcttc
     1921 aaatgtagca cctgaagtca gccccatacg atataagttg tatagtgctt ggattctcac
     1981 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     2041 tcattactgg atctatcaac aggagtccaa gcgagctctc gaaccccaga gtcccgctca
     2101 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     2161 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     2221 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     2281 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
     2341 gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag
     2401 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     2461 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     2521 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     2581 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     2641 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     2701 tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     2761 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     2821 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     2881 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatcttg atcccctgcg
     2941 ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt
     3001 accagagggc gccccagctg gcaattccga cgtctaagaa accattatta tcatgacatt
     3061 aacctataaa aataggcgta tcacgaggcc ctttcgtctt cacctcgaga tgctagc
//
