LOCUS       sequence-26079-e         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Czlapinski JL, Schelle MW, Miller LW, Laughlin ST, Kohler JJ, 
            Cornish VW, Bertozzi CR
  TITLE     Conditional glycosylation in eukaryotic cells using a biocompatible 
            chemical inducer of dimerization.
  JOURNAL   J Am Chem Soc. 2008 Oct 8. 130(40):13186-7.
  PUBMED    18788807
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2008 Oct 8. 130(40):13186-7."
COMMENT     Sequence Label: Vh-HA-FRB
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(83..361)
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             complement(362..388)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     sig_peptide     complement(join(422..433,516..560))
                     /codon_start=1
                     /product="modified signal sequence from the mouse 
                     immunoglobulin heavy chain variable region"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     CDS             complement(join(422..432,515..560))
                     /codon_start=1
                     /product="signal sequence from the mouse immunoglobulin 
                     heavy chain variableregion"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     regulatory      557..566
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(623..722)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(652..672)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gactcgagcg gccgccactg tgctggatat ctgcagaatt ccaccacact ggactagtgg
       61 atccgagctc ggtacctcta gtctttgaga ttcgtcggaa cacatgataa tagaggtccc
      121 aggcttggag gaggtccttg acattccctg atttcatgta cttcctgcac cactcttggg
      181 cctccattaa atctcgacca taggcctgat taaaggatgt ttccttcaga gtctgggggc
      241 cccgttccat catagcatgc aagggctcca gcacctcaaa catgcctttc acgttccttt
      301 ccccaaagta caaacgagat gcctcttcca ggccttcatg ccacatctca tgccagagga
      361 ttgcgtagtc tggtacgtcg tacggatatc tagactcgag gtcgacctgc agttggacct
      421 gggagtgcgc gcctgtggag agaaaggcaa agtggatgtc attgtcaccc atatatatgt
      481 ccagacctca agcctgctac tgttaacccc ttacctgtag ctgttgctac caagaagagg
      541 atgatacagc tccatcccat ggtggccacg tcgtattaat ttccacgtgc cagtaagcag
      601 tgggttctct agttagccag aaagctctgc ttatatagac ctcccaccgt acacgcctac
      661 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
      721 ggtgca
//
