LOCUS       sequence-260792-        3225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZE1-katGp-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: TJF64
FEATURES             Location/Qualifiers
     source          1..3225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             338..349
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             356..1066
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(501..519)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     769..788
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1102..1188
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1353..1941)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1433..1452)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2029..2123)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2153..2944,2945..3013))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2776..2795
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3014..3118)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3186..3204
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgtggctttt atgaaaatca cacagtgatc acaaatttta aacagagcac aaaatgctgc
       61 ctcgaaatga gggcgggaaa ataaggttat cagccttgtt ttctccctca ttacttgaag
      121 gatatgaagc taaaaccctt ttttataaag catttgtccg aattcggaca taatcaaaaa
      181 agcttaatta agatcaattt gatctacatc tctttaacca acaatatgta agatctcaac
      241 tatcgcatcc gtggattaat tcaattataa cttctctcta acgctgtgta tcgtaacggt
      301 aacactgtag aggggagcac attgatgcga attcattaaa gaggagaaag gtaccatggt
      361 gagcaagggc gaagaagata acatggccat catcaaggag ttcatgcgct tcaaggtgca
      421 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
      481 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
      541 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
      601 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
      661 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
      721 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
      781 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
      841 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
      901 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
      961 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     1021 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaacccg ggggatccca
     1081 tggtacgcgt gctagaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1141 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccctag
     1201 acctagggcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1261 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1321 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1381 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1441 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1501 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1561 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1621 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1681 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1741 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     1801 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1861 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1921 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1981 tggaacgaaa actcacgtta agggattttg gtcatgacta gtgcttggat tctcaccaat
     2041 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     2101 tactggatct atcaacagga gtccaagcga gctcgtaaac ttggtctgac agttaccaat
     2161 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2221 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2281 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2341 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2401 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2461 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2521 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2581 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2641 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2701 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2761 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2821 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2881 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2941 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3001 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3061 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3121 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     3181 ataaaaatag gcgtatcacg aggccctttc gtcttcacct cgagg
//
