LOCUS       sequence-260794-        4225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZE1-oxySp-mCherry-proD-oxyR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: TJF229
FEATURES             Location/Qualifiers
     source          1..4225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             111..122
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             129..839
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(274..292)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     542..561
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      875..961
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2104..2190
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2354..2942)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2434..2453)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3030..3124)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3154..3945,3946..4014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3777..3796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4015..4119)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4187..4205
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttcattatcc atcctccatc gccacgatag ttcatggcga taggtagaat agcaatgaac
       61 gattatccct atcaagcatt ctgactgata attgctcaca cgaattcatt aaagaggaga
      121 aaggtaccat ggtgagcaag ggcgaagaag ataacatggc catcatcaag gagttcatgc
      181 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
      241 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
      301 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
      361 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
      421 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
      481 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
      541 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
      601 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
      661 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
      721 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
      781 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaac
      841 ccgggggatc ccatggtacg cgtgctagag gcatcaaata aaacgaaagg ctcagtcgaa
      901 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
      961 tccgccgccc tagacctagc acagctaaca ccacgtcgtc cctatctgct gccctaggtc
     1021 tatgagtggt tgctggataa ctttacgggc atgcataagg ctcgtataat atattcaggg
     1081 agaccacaac ggtttccctc tacaaataat tttgtttaac tttgaattct tcacacagga
     1141 aaccggtacc atgaatattc gtgatcttga gtacctggtg gcattggctg aacaccgcca
     1201 ttttcggcgt gcggcagatt cctgccacgt tagccagccg acgcttagcg ggcaaattcg
     1261 taagctggaa gatgagctgg gcgtgatgtt gctggagcgg accagccgta aagtgttgtt
     1321 cacccaggcg ggaatgctgc tggtggatca ggcgcgtacc gtgctgcgtg aggtgaaagt
     1381 ccttaaagag atggcaagcc agcagggcga gacgatgtcc ggaccgctgc acattggttt
     1441 gattcccaca gttggaccgt acctgctacc gcatattatc cctatgctgc accagacctt
     1501 tccaaagctg gaaatgtatc tgcatgaagc acagacccac cagttactgg cgcaactgga
     1561 cagcggcaaa ctcgattgcg tgatcctcgc gctggtgaaa gagagcgaag cattcattga
     1621 agtgccgttg tttgatgagc caatgttgct ggctatctat gaagatcacc cgtgggcgaa
     1681 ccgcgaatgc gtaccgatgg ccgatctggc aggggaaaaa ctgctgatgc tggaagatgg
     1741 tcactgtttg cgcgatcagg caatgggttt ctgttttgaa gccggggcgg atgaagatac
     1801 acacttccgc gcgaccagcc tggaaactct gcgcaacatg gtggcggcag gtagcgggat
     1861 cactttactg ccagcgctgg ctgtgccgcc ggagcgcaaa cgcgatgggg ttgtttatct
     1921 gccgtgcatt aagccggaac cacgccgcac tattggcctg gtttatcgtc ctggctcacc
     1981 gctgcgcagc cgctatgagc agctggcaga ggccatccgc gcaagaatgg atggccattt
     2041 cgataaagtt ttaaaacagg cggtttaacc cgggggatcc catggtacgc gtgctagagg
     2101 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2161 tcggtgaacg ctctcctgag taggacaaat ccgccgccct agactagggc gttcggctgc
     2221 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2281 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2341 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2401 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2461 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2521 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2581 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2641 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2701 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2761 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     2821 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2881 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2941 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3001 aagggatttt ggtcatgact agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa
     3061 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
     3121 agtccaagcg agctcgtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3181 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3241 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3301 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3361 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3421 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3481 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3541 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3601 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3661 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3721 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3781 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3841 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3901 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3961 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4021 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     4081 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4141 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     4201 gaggcccttt cgtcttcacc tcgag
//
