LOCUS       sequence-260796-        3921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZE1-oxySp-oxyR-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: TJF205
FEATURES             Location/Qualifiers
     source          1..3921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             111..122
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1056..1763
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1198..1216)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1466..1485
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1799..1885
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2050..2638)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2130..2149)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2726..2820)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2850..3641,3642..3710))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3473..3492
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3711..3815)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3883..3901
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttcattatcc atcctccatc gccacgatag ttcatggcga taggtagaat agcaatgaac
       61 gattatccct atcaagcatt ctgactgata attgctcaca cgaattcatt aaagaggaga
      121 aaggtaccat gaatattcgt gatcttgagt acctggtggc attggctgaa caccgccatt
      181 ttcggcgtgc ggcagattcc tgccacgtta gccagccgac gcttagcggg caaattcgta
      241 agctggaaga tgagctgggc gtgatgttgc tggagcggac cagccgtaaa gtgttgttca
      301 cccaggcggg aatgctgctg gtggatcagg cgcgtaccgt gctgcgtgag gtgaaagtcc
      361 ttaaagagat ggcaagccag cagggcgaga cgatgtccgg accgctgcac attggtttga
      421 ttcccacagt tggaccgtac ctgctaccgc atattatccc tatgctgcac cagacctttc
      481 caaagctgga aatgtatctg catgaagcac agacccacca gttactggcg caactggaca
      541 gcggcaaact cgattgcgtg atcctcgcgc tggtgaaaga gagcgaagca ttcattgaag
      601 tgccgttgtt tgatgagcca atgttgctgg ctatctatga agatcacccg tgggcgaacc
      661 gcgaatgcgt accgatggcc gatctggcag gggaaaaact gctgatgctg gaagatggtc
      721 actgtttgcg cgatcaggca atgggtttct gttttgaagc cggggcggat gaagatacac
      781 acttccgcgc gaccagcctg gaaactctgc gcaacatggt ggcggcaggt agcgggatca
      841 ctttactgcc agcgctggct gtgccgccgg agcgcaaacg cgatggggtt gtttatctgc
      901 cgtgcattaa gccggaacca cgccgcacta ttggcctggt ttatcgtcct ggctcaccgc
      961 tgcgcagccg ctatgagcag ctggcagagg ccatccgcgc aagaatggat ggccatttcg
     1021 ataaagtttt aaaacaggcg gttggtggtt ctggtgtgag caagggcgaa gaagataaca
     1081 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     1141 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     1201 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     1261 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     1321 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     1381 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     1441 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     1501 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     1561 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     1621 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     1681 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     1741 gcatggacga gctgtacaag taacccgggg gatcccatgg tacgcgtgct agaggcatca
     1801 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1861 gaacgctctc ctgagtagga caaatccgcc gccctagacc tagggcgttc ggctgcggcg
     1921 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1981 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2041 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2101 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2161 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2221 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2281 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2341 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2401 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2461 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     2521 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2581 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2641 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2701 gattttggtc atgactagtg cttggattct caccaataaa aaacgcccgg cggcaaccga
     2761 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     2821 caagcgagct cgtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2881 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2941 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3001 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3061 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3121 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3181 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3241 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3301 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3361 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3421 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3481 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3541 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3601 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3661 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3721 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3781 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3841 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     3901 ccctttcgtc ttcacctcga g
//
