LOCUS       sequence-260797-        2664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZA2-pLlacO-soxR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: fIM1265
FEATURES             Location/Qualifiers
     source          1..2664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..19
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    26..42
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             94..105
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      606..692
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(729..746)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(863..1408)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(943..962)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1520..1614)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1645..2439)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1756..1775)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2366..2385
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2618..2636
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatcagc
       61 aggacgcact gaccttaatt aaaagaattc attaaagagg agaaaggtac catggaaaag
      121 aaattacccc gcattaaagc gctgctaacc cccggcgaag tggcgaaacg cagcggtgtg
      181 gcggtatcgg cgctgcattt ctatgaaagt aaagggttga ttaccagtat ccgtaacagc
      241 ggcaatcagc ggcgatataa acgtgatgtg ttgcgatatg ttgcaattat caaaattgct
      301 cagcgtattg gcattccgct ggcgaccatt ggtgaagcgt ttggcgtgtt gcccgaaggg
      361 catacgttaa gtgcgaaaga gtggaaacag ctttcgtccc aatggcgaga agagttggat
      421 cggcgcattc ataccttagt ggcgctgcgt gacgaactgg acggatgtat tggttgtggc
      481 tgcctttcgc gcagtgattg cccgttgcgt aacccgggcg accgcttagg agaagaaggt
      541 accggcgcac gcttgctgga agatgaacaa aactaaggat cccatggtac gcgtgctaga
      601 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      661 tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc ctagacctag ggggatatat
      721 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg
      781 gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg
      841 agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa
      901 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      961 cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
     1021 cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt
     1081 cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc
     1141 ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc
     1201 actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga
     1261 aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt
     1321 agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga
     1381 gattacgcgc agaccaaaac gatctcaaga agatcatctt attaatcaga taaaatattt
     1441 ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata
     1501 taagttgtat agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     1561 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
     1621 agctctcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat
     1681 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     1741 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
     1801 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     1861 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag
     1921 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     1981 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     2041 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     2101 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     2161 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     2221 cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga
     2281 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     2341 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     2401 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt
     2461 ctcttgatca gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca
     2521 gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca attccgacgt
     2581 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     2641 tcgtcttcac ctcgagatgc tagc
//
