LOCUS       sequence-260803-        3078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZA4-pLsoxS-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: pIM10
FEATURES             Location/Qualifiers
     source          1..3078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             79..90
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             97..807
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(242..260)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     510..529
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      843..929
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(966..983)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1100..1645)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1180..1199)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1765..1859)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1909..2700)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     3040..3058
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ccataaatcg ctttacctca agttaacttg aggaattata ctccccaaca tcagcaggac
       61 gcactgaccg aattcattaa agaggagaaa ggtaccatgg tgagcaaggg cgaagaagat
      121 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
      181 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      241 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
      301 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
      361 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
      421 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
      481 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
      541 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
      601 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
      661 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
      721 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
      781 ggcggcatgg acgagctgta caagtaaccc gggggatccc atggtacgcg tgctagaggc
      841 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      901 cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gacctagggg gatatattcc
      961 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct
     1021 tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga
     1081 gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct
     1141 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1201 ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
     1261 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
     1321 tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt
     1381 aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact
     1441 ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag
     1501 gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc
     1561 tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat
     1621 tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa aatatttcta
     1681 gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa
     1741 gttgttacta gtactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     1801 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     1861 aagcgagctc gagctcgata tccgtcggct tgaacgaatt gttagacatt atttgccgac
     1921 taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga
     1981 ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg
     2041 atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt
     2101 gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc
     2161 agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc
     2221 ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg
     2281 ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat
     2341 acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata
     2401 acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc
     2461 gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt
     2521 ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc
     2581 gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc
     2641 gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat
     2701 gatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa
     2761 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg
     2821 taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
     2881 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagctt
     2941 acgaaccgaa caggcttatg tccactgggt tcgtgccttc atccggatat cgacgtcgac
     3001 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     3061 tttcgtcttc acctcgag
//
