LOCUS       sequence-260804-        4234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZE1-oxySp-sfGFP-proD-oxyR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: pIM09
FEATURES             Location/Qualifiers
     source          1..4234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             111..122
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             129..842
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      884..970
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2113..2199
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2363..2951)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2443..2462)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3039..3133)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3163..3954,3955..4023))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3786..3805
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4024..4128)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4196..4214
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttcattatcc atcctccatc gccacgatag ttcatggcga taggtagaat agcaatgaac
       61 gattatccct atcaagcatt ctgactgata attgctcaca cgaattcatt aaagaggaga
      121 aaggtaccat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt ctggtggaac
      181 tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa ggtgacgcaa
      241 ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg gtaccttggc
      301 cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat ccggaccata
      361 tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag gaacgcacga
      421 tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt gaaggcgata
      481 ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaagacggc aatatcctgg
      541 gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc gataaacaaa
      601 aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc agcgtgcagc
      661 tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg ctgccagaca
      721 atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa cgcgatcata
      781 tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat gaactgtaca
      841 aatgatgacc cgggggatcc catggtacgc gtgctagagg catcaaataa aacgaaaggc
      901 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
      961 taggacaaat ccgccgccct agacctagca cagctaacac cacgtcgtcc ctatctgctg
     1021 ccctaggtct atgagtggtt gctggataac tttacgggca tgcataaggc tcgtataata
     1081 tattcaggga gaccacaacg gtttccctct acaaataatt ttgtttaact ttgaattctt
     1141 cacacaggaa accggtacca tgaatattcg tgatcttgag tacctggtgg cattggctga
     1201 acaccgccat tttcggcgtg cggcagattc ctgccacgtt agccagccga cgcttagcgg
     1261 gcaaattcgt aagctggaag atgagctggg cgtgatgttg ctggagcgga ccagccgtaa
     1321 agtgttgttc acccaggcgg gaatgctgct ggtggatcag gcgcgtaccg tgctgcgtga
     1381 ggtgaaagtc cttaaagaga tggcaagcca gcagggcgag acgatgtccg gaccgctgca
     1441 cattggtttg attcccacag ttggaccgta cctgctaccg catattatcc ctatgctgca
     1501 ccagaccttt ccaaagctgg aaatgtatct gcatgaagca cagacccacc agttactggc
     1561 gcaactggac agcggcaaac tcgattgcgt gatcctcgcg ctggtgaaag agagcgaagc
     1621 attcattgaa gtgccgttgt ttgatgagcc aatgttgctg gctatctatg aagatcaccc
     1681 gtgggcgaac cgcgaatgcg taccgatggc cgatctggca ggggaaaaac tgctgatgct
     1741 ggaagatggt cactgtttgc gcgatcaggc aatgggtttc tgttttgaag ccggggcgga
     1801 tgaagataca cacttccgcg cgaccagcct ggaaactctg cgcaacatgg tggcggcagg
     1861 tagcgggatc actttactgc cagcgctggc tgtgccgccg gagcgcaaac gcgatggggt
     1921 tgtttatctg ccgtgcatta agccggaacc acgccgcact attggcctgg tttatcgtcc
     1981 tggctcaccg ctgcgcagcc gctatgagca gctggcagag gccatccgcg caagaatgga
     2041 tggccatttc gataaagttt taaaacaggc ggtttaaccc gggggatccc atggtacgcg
     2101 tgctagaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     2161 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gactagggcg
     2221 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2281 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2341 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2401 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2461 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2521 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2581 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2641 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2701 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2761 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     2821 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2881 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2941 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3001 actcacgtta agggattttg gtcatgacta gtgcttggat tctcaccaat aaaaaacgcc
     3061 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     3121 atcaacagga gtccaagcga gctcgtaaac ttggtctgac agttaccaat gcttaatcag
     3181 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3241 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3301 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3361 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3421 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3481 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3541 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3601 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3661 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3721 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3781 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3841 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3901 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3961 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4021 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4081 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4141 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     4201 gcgtatcacg aggccctttc gtcttcacct cgag
//
