LOCUS       sequence-260807-        5048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZS2-pLsoxS-TEVp-proD-soxR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: pIM04
FEATURES             Location/Qualifiers
     source          1..5048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             79..90
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             100..807
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPE
                     EPFQPVKEATQLMN"
     terminator      861..947
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1638..1724
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2269..3219)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3267..3489
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3987..4081)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4112..4906)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4223..4242)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4833..4852
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ccataaatcg ctttacctca agttaacttg aggaattata ctccccaaca tcagcaggac
       61 gcactgaccg aattcattaa agaggagaaa ggtaccatgg gagaaagctt gtttaagggg
      121 ccgcgtgatt acaacccgat atcgagcacc atttgtcatt tgacgaatga atctgatggg
      181 cacacaacat cgttgtatgg tattggattt ggtcccttca tcattacaaa caagcacttg
      241 tttagaagaa ataatggaac actgttggtc caatcactac atggtgtatt caaggtcaag
      301 aacaccacga ctttgcaaca acacctcatt gatgggaggg acatgataat tattcgcatg
      361 cctaaggatt tcccaccatt tcctcaaaag ctgaaattta gagagccaca aagggaagag
      421 cgcatatgtc ttgtgacaac caacttccaa actaagagca tgtctagcat ggtgtcagac
      481 actagttgca cattcccttc atctgatggc atattctgga agcattggat tcaaaccaag
      541 gatgggcagt gtggcagtcc attagtatca actagagatg ggttcattgt tggtatacac
      601 tcagcatcga atttcaccaa cacaaacaat tatttcacaa gcgtgccgaa aaacttcatg
      661 gaattgttga caaatcagga ggcgcagcag tgggttagtg gttggcgatt aaatgctgac
      721 tcagtattgt gggggggcca taaagttttc atggtgaaac ctgaagagcc ttttcagcca
      781 gttaaggaag cgactcaact catgaatcgt cgtcgccgtc gctaacccgg gggatcccat
      841 ggtacgcgtg ctagaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      901 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga
      961 gctagccaca gctaacacca cgtcgtccct atctgctgcc ctaggtctat gagtggttgc
     1021 tggataactt tacgggcatg cataaggctc gtataatata ttcagggaga ccacaacggt
     1081 ttccctctac aaataatttt gtttaacttt gaattcttca cacaggaaac cggtaccatg
     1141 gaaaagaaat taccccgcat taaagcgctg ctaacccccg gcgaagtggc gaaacgcagc
     1201 ggtgtggcgg tatcggcgct gcatttctat gaaagtaaag ggttgattac cagtatccgt
     1261 aacagcggca atcagcggcg atataaacgt gatgtgttgc gatatgttgc aattatcaaa
     1321 attgctcagc gtattggcat tccgctggcg accattggtg aagcgtttgg cgtgttgccc
     1381 gaagggcata cgttaagtgc gaaagagtgg aaacagcttt cgtcccaatg gcgagaagag
     1441 ttggatcggc gcattcatac cttagtggcg ctgcgtgacg aactggacgg atgtattggt
     1501 tgtggctgcc tttcgcgcag tgattgcccg ttgcgtaacc cgggcgaccg cttaggagaa
     1561 gaaggtaccg gcgcacgctt gctggaagat gaacaaaact aacccggggg atcccatggt
     1621 acgcgtgcta gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1681 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ccctagacct
     1741 agggcctagg gtacgggttt tgctgcccgc aaacgggctg ttctggtgtt gctagtttgt
     1801 tatcagaatc gcagatccgg cttcagccgg tttgccggct gaaagcgcta tttcttccag
     1861 aattgccatg attttttccc cacgggaggc gtcactggct cccgtgttgt cggcagcttt
     1921 gattcgataa gcagcatcgc ctgtttcagg ctgtctatgt gtgactgttg agctgtaaca
     1981 agttgtctca ggtgttcaat ttcatgttct agttgctttg ttttactggt ttcacctgtt
     2041 ctattaggtg ttacatgctg ttcatctgtt acattgtcga tctgttcatg gtgaacagct
     2101 ttgaatgcac caaaaactcg taaaagctct gatgtatcta tcttttttac accgttttca
     2161 tctgtgcata tggacagttt tccctttgat atgtaacggt gaacagttgt tctacttttg
     2221 tttgttagtc ttgatgcttc actgatagat acaagagcca taagaacctc agatccttcc
     2281 gtatttagcc agtatgttct ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga
     2341 accattgaga tcatacttac tttgcatgtc actcaaaaat tttgcctcaa aactggtgag
     2401 ctgaattttt gcagttaaag catcgtgtag tgtttttctt agtccgttat gtaggtagga
     2461 atctgatgta atggttgttg gtattttgtc accattcatt tttatctggt tgttctcaag
     2521 ttcggttacg agatccattt gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg
     2581 gcggcctcgc ttatcaacca ccaatttcat attgctgtaa gtgtttaaat ctttacttat
     2641 tggtttcaaa acccattggt taagcctttt aaactcatgg tagttatttt caagcattaa
     2701 catgaactta aattcatcaa ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt
     2761 tagttctttt aataaccact cataaatcct catagagtat ttgttttcaa aagacttaac
     2821 atgttccaga ttatatttta tgaatttttt taactggaaa agataaggca atatctcttc
     2881 actaaaaact aattctaatt tttcgcttga gaacttggca tagtttgtcc actggaaaat
     2941 ctcaaagcct ttaaccaaag gattcctgat ttccacagtt ctcgtcatca gctctctggt
     3001 tgctttagct aatacaccat aagcattttc cctactgatg ttcatcatct gagcgtattg
     3061 gttataagtg aacgataccg tccgttcttt ccttgtaggg ttttcaatcg tggggttgag
     3121 tagtgccaca cagcataaaa ttagcttggt ttcatgctcc gttaagtcat agcgactaat
     3181 cgctagttca tttgctttga aaacaactaa ttcagacata catctcaatt ggtctaggtg
     3241 attttaatca ctataccaat tgagatgggc tagtcaatga taattactag tccttttcct
     3301 ttgagttgtg ggtatctgta aattctgcta gacctttgct ggaaaacttg taaattctgc
     3361 tagaccctct gtaaattccg ctagaccttt gtgtgttttt tttgtttata ttcaagtggt
     3421 tataatttat agaataaaga aagaataaaa aaagataaaa agaatagatc ccagccctgt
     3481 gtataactca ctactttagt cagttccgca gtattacaaa aggatgtcgc aaacgctgtt
     3541 tgctcctcta caaaacagac cttaaaaccc taaaggctta agtagcaccc tcgcaagctc
     3601 gggcaaatcg ctgaatattc cttttgtctc cgaccatcag gcacctgagt cgctgtcttt
     3661 ttcgtgacat tcagttcgct gcgctcacgg ctctggcagt gaatgggggt aaatggcact
     3721 acaggcgcct tttatggatt catgcaagga aactacccat aatacaagaa aagcccgtca
     3781 cgggcttctc agggcgtttt atggcgggtc tgctatgtgg tgctatctga ctttttgctg
     3841 ttcagcagtt cctgccctct gattttccag tctgaccact tcggattatc ccgtgacagg
     3901 tcattcagac tggctaatgc acccagtaag gcagcggtat catcaacagg cttacccgtc
     3961 ttactgtccc tagtactagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     4021 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     4081 ccaagcgagc tctcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga
     4141 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
     4201 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
     4261 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
     4321 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg
     4381 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
     4441 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
     4501 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
     4561 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
     4621 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
     4681 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg
     4741 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca
     4801 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca
     4861 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc
     4921 atcctgtctc ttgatcagat cttgatcccc tgcgccatca gatccttggc ggcaagaaag
     4981 ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt
     5041 ccgacgtc
//
