LOCUS       sequence-260809-        3093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZA4-oxySp-mCherry-TEVrs-LAA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: pIM23
FEATURES             Location/Qualifiers
     source          1..3093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             111..122
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             129..836
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(274..292)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     542..561
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             843..863
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(1062..1079)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1196..1741)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1276..1295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1861..1955)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2005..2796)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttcattatcc atcctccatc gccacgatag ttcatggcga taggtagaat agcaatgaac
       61 gattatccct atcaagcatt ctgactgata attgctcaca cgaattcatt aaagaggaga
      121 aaggtaccat ggtgagcaag ggcgaagaag ataacatggc catcatcaag gagttcatgc
      181 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
      241 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
      301 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
      361 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
      421 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
      481 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
      541 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
      601 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
      661 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
      721 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
      781 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaaggtcg
      841 acgaaaacct gtacttccag ggtaggcctg cagcaaacga cgaaaactac gctttagcag
      901 cttaacccgg gggatcccat ggtacgcgtg ctagaggcat caaataaaac aaggctcagt
      961 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     1021 caaatccgcc gccctagacc tagggggata tattccgctt cctcgctcac tgactcgcta
     1081 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
     1141 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
     1201 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
     1261 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
     1321 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
     1381 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
     1441 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1501 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
     1561 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
     1621 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
     1681 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
     1741 agaagatcat cttattaatc agataaaata tttctagatt tcagtgcaat ttatctcttc
     1801 aaatgtagca cctgaagtca gccccatacg atataagttg ttactagtac tagtgcttgg
     1861 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     1921 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgagc tcgatatccg
     1981 tcggcttgaa cgaattgtta gacattattt gccgactacc ttggtgatct cgcctttcac
     2041 gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc
     2101 aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat
     2161 tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat
     2221 gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca
     2281 tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag
     2341 ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat
     2401 agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg
     2461 ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg
     2521 cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt
     2581 ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt
     2641 aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa
     2701 atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag
     2761 ttgagtcgat acttcggcga tcaccgcttc cctcatgatg tttaactttg ttttagggcg
     2821 actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta
     2881 acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac agtcataaca
     2941 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
     3001 gttgcgtgag cgcatacgct acttgcatta cagcttacga accgaacagg cttatgtcca
     3061 ctgggttcgt gccttcatcc ggatatcgac gtc
//
