LOCUS       sequence-26082-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Czlapinski JL, Schelle MW, Miller LW, Laughlin ST, Kohler JJ, 
            Cornish VW, Bertozzi CR
  TITLE     Conditional glycosylation in eukaryotic cells using a biocompatible 
            chemical inducer of dimerization.
  JOURNAL   J Am Chem Soc. 2008 Oct 8. 130(40):13186-7.
  PUBMED    18788807
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2008 Oct 8. 130(40):13186-7."
COMMENT     Sequence Label: FRB-myc
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..47)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(48..326)
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     primer_bind     complement(441..460)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(442..460)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(505..708)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(534..554)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aacgggcctc tagtctacag atcctcttct gagatgagtt tttgttcctt tgagattcgt
       61 cggaacacat gataatagag gtcccaggct tggaggaggt ccttgacatt ccctgatttc
      121 atgtacttcc tgcaccactc ttgggcctcc attaaatctc gaccataggc ctgattaaag
      181 gatgtttcct tcagagtctg ggggccccgt tccatcatag catgcaaggg ctccagcacc
      241 tcaaacatgc ctttcacgtt cctttcccca aagtacaaac gagatgcctc ttccaggcct
      301 tcatgccaca tctcatgcca gaggattcta gactcgagcg gccgccactg tgctggatat
      361 ctgcagaatt ccaccacact ggactagtgg atccgagctc ggtaccaagc ttaagtttaa
      421 acgctagcca gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc
      481 agtgggttct ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct
      541 accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt
      601 ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga
      661 gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc
      721 gatgactaat acgtagatgt actgccanta ganaaaannc cnatanggtc cntnnncnng
      781 gcatnannnn nnngggggcc annacccgca nnnagnncnn nna
//
