LOCUS       sequence-260830-       12226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of xFNLS in mammalian 
            cells (codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 12226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-xFNLS-P2A-Puro
FEATURES             Location/Qualifiers
     source          1..12226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    125..242
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        311..522
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      542..551
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             551..616
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             623..643
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             668..1354
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             1403..5503
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 mutant with broad PAM
                     compatibility (Hu et al., 2018)"
                     /label=xCas9-3.7
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1403..5503
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5516..5764
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5777..5797
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5813..5869
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5870..6469
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5870..5889)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6366..6386
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6485..7073
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6538..6558)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6956..6967)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7145..7378
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7456..7577
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7493..7512)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7547..7566
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7617..7752
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7772..7791)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7773..7791)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7801..7818)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7801..7817)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7810..7832)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7959..8414
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8046..8065)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8256..8277
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8440..8544
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8545..8613,8614..9405)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8763..8782)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9576..10164
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10065..10084
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10318..10335
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10452..10473
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10488..10493,10494..10511,10512..10518)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10526..10542
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10531..10553
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10550..10566
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10550..10566
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10585..10605
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10587..10605
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10633..10859
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10860..11040
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11087..11212
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11705..11938
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12123..12167
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
       61 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccagacc
      121 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
      181 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
      241 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag tggctccggt
      301 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
      361 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
      421 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
      481 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtgtcgtg acgcgggatc
      541 cgccaccatg gactataagg accacgacgg agactacaag gatcatgata ttgattacaa
      601 agacgatgac gataagatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc
      661 agcagccatg agctcagaga ctggcccagt ggctgtggac cccacattga gacggcggat
      721 cgagccccat gagtttgagg tattcttcga tccgagagag ctccgcaagg agacctgcct
      781 gctttacgaa attaattggg ggggccggca ctccatttgg cgacatacat cacagaacac
      841 taacaagcac gtcgaagtca acttcatcga gaagttcacg acagaaagat atttctgtcc
      901 gaacacaagg tgcagcatta cctggtttct cagctggagc ccatgcggcg aatgtagtag
      961 ggccatcact gaattcctgt caaggtatcc ccacgtcact ctgtttattt acatcgcaag
     1021 gctgtaccac cacgctgacc cccgcaatcg acaaggcctg cgggatttga tctcttcagg
     1081 tgtgactatc caaattatga ctgagcagga gtcaggatac tgctggagaa actttgtgaa
     1141 ttatagcccg agtaatgaag cccactggcc taggtatccc catctgtggg tacgactgta
     1201 cgttcttgaa ctgtactgca tcatactggg cctgcctcct tgtctcaaca ttctgagaag
     1261 gaagcagcca cagctgacat tctttaccat cgctcttcag tcttgtcatt accagcgact
     1321 gcccccacac attctctggg ccaccgggtt gaaaagcggc agcgagactc ccgggacctc
     1381 agagtccgcc acacccgaaa gtgacaagaa gtacagcatc ggcctggcca tcggcaccaa
     1441 ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt
     1501 gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga
     1561 cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag
     1621 acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga
     1681 cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga
     1741 gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac
     1801 catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat
     1861 ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct
     1921 gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca
     1981 gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc
     2041 cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa
     2101 gaatggcctg ttcggaaacc tgattgccct gagcctgggc ctgaccccca acttcaagag
     2161 caacttcgac ctggccgagg ataccaaact gcagctgagc aaggacacct acgacgacga
     2221 cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa
     2281 gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa
     2341 ggcccccctg agcgcctcta tgatcaagct gtacgacgag caccaccagg acctgaccct
     2401 gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca
     2461 gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa
     2521 gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa
     2581 cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcatcatcc cccaccagat
     2641 ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa
     2701 ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc
     2761 tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac
     2821 cccctggaac ttcgagaagg tggtggacaa gggcgcttcc gcccagagct tcatcgagcg
     2881 gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct
     2941 gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat
     3001 gagaaagccc gccttcctga gcggcgacca gaaaaaggcc atcgtggacc tgctgttcaa
     3061 gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg
     3121 cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata
     3181 ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga
     3241 cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga
     3301 acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg
     3361 gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca
     3421 gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat
     3481 ccagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc
     3541 cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa
     3601 gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca
     3661 caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca
     3721 gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca
     3781 gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta
     3841 ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc
     3901 cgactacgat gtggaccata tcgtgcctca gagctttctg aaggacgact ccatcgacaa
     3961 caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga
     4021 ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca
     4081 gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc
     4141 cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat
     4201 cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa
     4261 agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa
     4321 agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg
     4381 aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa
     4441 ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc
     4501 caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa
     4561 cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg
     4621 ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat
     4681 cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag
     4741 gaacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt
     4801 cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc
     4861 caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt
     4921 cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct
     4981 gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct
     5041 ggcctctgcc ggcgtgctgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa
     5101 cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca
     5161 gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag
     5221 cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta
     5281 caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac
     5341 cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa
     5401 gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg
     5461 cctgtacgag acacggatcg acctgtctca gctgggaggc gattcaggcg gatctactaa
     5521 tctgtcagat attattgaaa aggagaccgg taagcaactg gttatccagg aatccatcct
     5581 catgctccca gaggaggtgg aagaagtcat tgggaacaag ccggaaagcg atatactcgt
     5641 gcacaccgcc tacgacgaga gcaccgacga gaatgtcatg cttctgacta gcgacgcccc
     5701 tgaatacaag ccttgggctc tggtcataca ggatagcaac ggtgagaaca agattaagat
     5761 gctctctggt ggttctccca agaagaagag gaaagtcgct agcggcagcg gcgccaccaa
     5821 cttcagcctg ctgaagcagg ccggcgacgt ggaggagaac cccggcccca tgaccgagta
     5881 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     5941 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     6001 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     6061 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     6121 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     6181 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     6241 cctggccacc gtcggagtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     6301 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     6361 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     6421 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttaagt
     6481 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6541 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6601 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6661 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6721 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6781 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     6841 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     6901 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     6961 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     7021 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
     7081 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     7141 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     7201 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7261 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7321 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7381 acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7441 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7501 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7561 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     7621 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7681 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     7741 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     7801 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     7861 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     7921 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     7981 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     8041 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     8101 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     8161 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     8221 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     8281 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     8341 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     8401 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     8461 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     8521 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     8581 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     8641 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     8701 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     8761 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     8821 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     8881 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     8941 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     9001 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     9061 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     9121 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     9181 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     9241 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     9301 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     9361 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     9421 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     9481 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     9541 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tttgcgcgta
     9601 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     9661 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     9721 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     9781 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     9841 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     9901 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     9961 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    10021 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    10081 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    10141 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    10201 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    10261 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    10321 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
    10381 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
    10441 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
    10501 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
    10561 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
    10621 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
    10681 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
    10741 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
    10801 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
    10861 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
    10921 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
    10981 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
    11041 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
    11101 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
    11161 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
    11221 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
    11281 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
    11341 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
    11401 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
    11461 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
    11521 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
    11581 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
    11641 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
    11701 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
    11761 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
    11821 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
    11881 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
    11941 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
    12001 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
    12061 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
    12121 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
    12181 acataacaaa ttggctgtgg tatataaaat tattcataat gatagt
//
