LOCUS       sequence-260838-       10925 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of IRF4-3xHA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 10925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-UBC- IRF4-3xHA-pGK-PUR
FEATURES             Location/Qualifiers
     source          1..10925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    181..298
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        390..1601
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1438..1457
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    1649..1673
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3027..3051)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3098..3187
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        3229..3728
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3264..3287)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3661..3680
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3749..4348
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3749..3768)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4245..4265
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(4379..4412)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4468..5056
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4521..4541)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4939..4950)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5059..5075)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5060..5076)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5265..5498
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5570..5691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5607..5626)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5661..5680
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5731..5866
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5886..5905)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5887..5905)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5928..6286)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5968..5989)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6180..6199
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6313..6417
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6419..6776
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6419..6610
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6442..6462)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6627..6762
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6811..7605
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6811..7605
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6865..6884)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7475..7494
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7793..7812)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7837..7884
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8213..8801
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8702..8721
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8976..9279
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9281..9479
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9434..9454
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9497..9677
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9724..9849
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10346..10579
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10764..10808
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
       61 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
      121 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
      181 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
      241 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      301 ggtttattac agggacagca gagatccagt ttggttagta ccgggcccgc tctagcccta
      361 gatttaaccc aacagaaggc tcgacgcgtg gcctccgcgc cgggttttgg cgcctcccgc
      421 gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc
      481 tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga
      541 accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt
      601 tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga
      661 gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca
      721 gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg
      781 tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc
      841 ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg
      901 ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga
      961 atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg
     1021 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga
     1081 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc
     1141 ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag tgcacccgta
     1201 cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg
     1261 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg
     1321 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg
     1381 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga
     1441 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg
     1501 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat
     1561 tgtccgctaa attctggccg tttttggctt ttttgttaga cgctagactg ccggatcgaa
     1621 ttgtcgacga attcggatct ggccaatcac aagtttgtac aaaaaagttg gcatgaacct
     1681 ggagggcggc ggccgaggcg gagagttcgg catgagcgcg gtgagctgcg gcaacgggaa
     1741 gctccgccag tggctgatcg accagatcga cagcggcaag taccccgggc tggtgtggga
     1801 gaacgaggag aagagcatct tccgcatccc ctggaagcac gcgggcaagc aggactacaa
     1861 ccgcgaggag gacgccgcgc tcttcaaggc ttgggcactg tttaaaggaa agttccgaga
     1921 aggcatcgac aagccggacc ctcccacctg gaagacgcgc ctgcggtgcg ctttgaacaa
     1981 gagcaatgac tttgaggaac tggttgagcg gagccagctg gacatctcag acccgtacaa
     2041 agtgtacagg attgttcctg agggagccaa aaaaggagcc aagcagctca ccttggagga
     2101 cccgcagatg tccatgagcc acccctacac catgacaacg ccttaccctt cgctcccagc
     2161 ccagcaggtt cacaactaca tgatgccacc cctcgaccga agctggaggg actacgtccc
     2221 ggatcagcca cacccggaaa tcccgtacca atgtcccatg acgtttggac cccgcggcca
     2281 ccactggcaa ggcccagctt gtgaaaatgg ttgccaggtg acaggaacct tttatgcttg
     2341 tgccccacct gagtcccagg ctcccggagt ccccacagag ccaagcataa ggtctgccga
     2401 agccttggcg ttctcagact gccggctgca catctgcctg tactaccggg aaatcctcgt
     2461 gaaggagctg accacgtcca gccccgaggg ctgccggatc tcccatggac atacgtatga
     2521 cgccagcaac ctggaccagg tcctgttccc ctacccagag gacaatggcc agaggaaaaa
     2581 cattgagaag ctgctgagcc acctggagag gggcgtggtc ctctggatgg cccccgacgg
     2641 gctctatgcg aaaagactgt gccagagcag gatctactgg gacgggcccc tggcgctgtg
     2701 caacgaccgg cccaacaaac tggagagaga ccagacctgc aagctctttg acacacagca
     2761 gttcttgtca gagctgcaag cgtttgctca ccacggccgc tccctgccaa gattccaggt
     2821 gactctatgc tttggagagg agtttccaga ccctcagagg caaagaaagc tcatcacagc
     2881 tcacgtagaa cctctgctag ccagacaact atattatttt gctcaacaaa acagtggaca
     2941 tttcctgagg ggctacgatt taccagaaca catcagcaat ccagaagatt accacagatc
     3001 tatccgccat tcctctattc aagaatgccc aactttcttg tacaaagtgg tgggtggagg
     3061 cggttcaggt ggcggaggaa gcggaggcgg tggaagttac ccctacgacg tgcccgacta
     3121 cgccggctat ccgtatgatg tcccggacta tgcaggatcc tatccatacg acgttccaga
     3181 ttacgcttag ttaattaagg ccggccctcg aggttaacga attctaccgg gtaggggagg
     3241 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     3301 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     3361 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     3421 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     3481 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     3541 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     3601 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     3661 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     3721 gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     3781 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     3841 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     3901 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     3961 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     4021 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     4081 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     4141 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     4201 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     4261 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     4321 gtgcatgacc cgcaagcccg gtgcctgatg tacaagtagg aattcgtcga gggacctaat
     4381 aacttcgtat agcatacatt atacgaagtt atacatgttt aagggttccg gttccactag
     4441 gtacaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     4501 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     4561 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     4621 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     4681 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     4741 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     4801 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     4861 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     4921 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     4981 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     5041 tgggccgcct ccccgcatcg ataccgtcga cctcgatcga gacctagaaa aacatggagc
     5101 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     5161 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     5221 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5281 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     5341 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5401 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5461 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     5521 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     5581 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5641 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5701 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5761 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5821 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     5881 attcgcccta tagtgagtcg tattagcatg tgagcaaaag gccagcataa atcaaaagaa
     5941 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     6001 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     6061 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     6121 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     6181 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     6241 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
     6301 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6361 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct
     6421 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     6481 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     6541 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6601 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6661 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     6721 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc
     6781 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     6841 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     6901 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     6961 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     7021 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     7081 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     7141 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     7201 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     7261 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     7321 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     7381 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     7441 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     7501 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     7561 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     7621 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     7681 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     7741 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac
     7801 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
     7861 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     7921 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     7981 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     8041 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat
     8101 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     8161 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8221 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8281 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8341 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8401 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     8461 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8521 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8581 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8641 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8701 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8761 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8821 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8881 ctgattctgt ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg
     8941 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     9001 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     9061 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     9121 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     9181 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     9241 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     9301 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     9361 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     9421 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc
     9481 gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     9541 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     9601 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     9661 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     9721 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     9781 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     9841 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     9901 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     9961 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
    10021 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
    10081 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
    10141 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga
    10201 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
    10261 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
    10321 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
    10381 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
    10441 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
    10501 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
    10561 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
    10621 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
    10681 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
    10741 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
    10801 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
    10861 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
    10921 gagtt
//
