LOCUS       sequence-260860-       11670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral gateway destination vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11670)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-UBC-HA-gate-pGK-HYG
FEATURES             Location/Qualifiers
     source          1..11670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    122..239
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        331..1542
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1379..1398
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1597..1623
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1645..1769
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1794..1799,1800..1817,1818..1824)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1878..2558
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(1944..1963)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2878..3183
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3062..3081
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3224..3348)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3492..3991
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3527..3550)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3924..3943
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4009..5034
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4009..5034
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(5065..5098)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5154..5742
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5207..5227)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5625..5636)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5745..5761)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5746..5762)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5951..6184
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6256..6377
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6293..6312)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6347..6366
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6417..6552
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6572..6591)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6573..6591)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6614..6972)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6654..6675)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6866..6885
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6999..7103
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7105..7462
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7105..7296
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7128..7148)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7313..7448
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7497..8291
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7497..8291
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7551..7570)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8161..8180
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8479..8498)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8523..8570
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8899..9487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9388..9407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9662..9965
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9967..10165
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10120..10140
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10183..10363
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10410..10535
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11032..11265
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11450..11494
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
       61 agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca
      121 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
      181 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
      241 gggtttatta cagggacagc agagatccag tttggttagt accgggcccg ctctagccct
      301 agatttaacc caacagaagg ctcgacgcgt ggcctccgcg ccgggttttg gcgcctcccg
      361 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc
      421 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag
      481 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg
      541 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg
      601 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac
      661 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc
      721 gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct
      781 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag
      841 gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg
      901 aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg
      961 ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg
     1021 agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga ctggagaact
     1081 cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca gtgcacccgt
     1141 acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat
     1201 gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag
     1261 ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg gtgaggggag
     1321 ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct taagtagctg
     1381 aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga agttttttag
     1441 gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa
     1501 ttgtccgcta aattctggcc gtttttggct tttttgttag acgctagact gccggatcga
     1561 attgtcgacg aattcggatc cagatctgcc accatgtacc catacgatgt tccagattac
     1621 gctggcggag ctcgaggtta tcaaacaagt ttgtacaaaa aagctgaacg agaaacgtaa
     1681 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
     1741 tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac
     1801 tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag
     1861 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     1921 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     1981 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     2041 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta
     2101 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     2161 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     2221 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     2281 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
     2341 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     2401 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     2461 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     2521 ggcaggcggg gcgtaatcta gaggatccgg cttactaaaa gccagataac agtatgcgta
     2581 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc
     2641 aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag
     2701 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg
     2761 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
     2821 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg
     2881 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
     2941 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
     3001 ctgtcagata aagtcccccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
     3061 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
     3121 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     3181 taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg
     3241 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     3301 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttc gataacgaat
     3361 tccgcccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     3421 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg ccgcgtttaa
     3481 cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     3541 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     3601 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     3661 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     3721 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     3781 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     3841 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     3901 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     3961 gttctcctct tcctcatctc cgggcctttc gacctgcatc ccgccaccat gaaaaagcct
     4021 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     4081 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     4141 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     4201 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     4261 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     4321 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     4381 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     4441 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     4501 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     4561 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     4621 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     4681 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     4741 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
     4801 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     4861 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     4921 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     4981 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagtgtaca
     5041 agtaggaatt cgtcgaggga cctaataact tcgtatagca tacattatac gaagttatac
     5101 atgtttaagg gttccggttc cactaggtac aattcgatat caagcttatc gataatcaac
     5161 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     5221 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     5281 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     5341 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     5401 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     5461 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     5521 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     5581 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     5641 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     5701 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     5761 gatcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     5821 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     5881 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     5941 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     6001 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6061 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6121 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6181 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     6241 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6301 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6361 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     6421 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     6481 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     6541 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt agcatgtgag
     6601 caaaaggcca gcataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     6661 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     6721 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     6781 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     6841 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     6901 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     6961 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     7021 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7081 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     7141 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7201 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7261 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7321 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7381 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7441 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     7501 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     7561 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     7621 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     7681 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     7741 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     7801 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     7861 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     7921 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     7981 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     8041 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     8101 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     8161 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     8221 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     8281 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     8341 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8401 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8461 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     8521 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     8581 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     8641 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     8701 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     8761 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8821 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8881 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8941 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9001 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9061 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9121 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9181 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9241 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9301 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9361 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9421 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9481 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9541 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
     9601 ttagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     9661 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     9721 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     9781 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     9841 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     9901 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     9961 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
    10021 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
    10081 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
    10141 tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac
    10201 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
    10261 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
    10321 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
    10381 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
    10441 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
    10501 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
    10561 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
    10621 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
    10681 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
    10741 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
    10801 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
    10861 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
    10921 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
    10981 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
    11041 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    11101 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    11161 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    11221 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    11281 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    11341 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    11401 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    11461 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    11521 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    11581 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    11641 gacccacctc ccaaccccga ggggacccga
//
