LOCUS       sequence-260867-       12186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1
            promoter and bar gene for plant selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12186)
  AUTHORS   Lee K, Zhang Y, Kleinstiver BP, Guo JA, Aryee MJ, Miller J, Malzahn 
            A, Zarecor S, Lawrence-Dill CJ, Joung JK, Qi Y, Wang K
  TITLE     Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for
            targeted mutagenesis in maize.
  JOURNAL   Plant Biotechnol J. 2018 Jul 4. doi: 10.1111/pbi.12982.
  PUBMED    29972722
REFERENCE   2  (bases 1 to 12186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2018 Jul 4. doi: 10.1111/pbi.12982."
COMMENT     Sequence Label: pYPQ210
FEATURES             Location/Qualifiers
     source          1..12186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    283..307
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     495..517
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     509..526
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     510..526
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        542..2539
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    2582..2706
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2731..2736,2737..2754,2755..2761)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2815..3495
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(2881..2900)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3815..4120
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3999..4018
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4161..4285)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4353..4369)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4353..4369)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4366..4388)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4377..4393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4401..4407,4408..4425,4426..4431))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4446..4467
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4658..5334
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     5271..5291
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             5379..5930
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    5937..6111
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6189..6213
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6638..7432
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7519..8107
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8008..8027
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8261..8278
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8293..8433
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8359..8378)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8519..8541
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8777..8971
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9037..10110)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10539..11168)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
       61 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
      121 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg
      181 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
      241 tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat
      301 cctgtcaaac actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa
      361 gctgctctag cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
      421 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      481 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttgcatg
      541 cctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct
      601 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct
      661 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata
      721 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg
      781 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt
      841 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag
      901 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt
      961 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat
     1021 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa
     1081 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg
     1141 acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag
     1201 acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg
     1261 gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca
     1321 cggcaggcgg cctcctcctc ctctcacggc accggcagct acgggggatt cctttcccac
     1381 cgctccttcg ctttcccttc ctcgcccgcc gtaataaata gacaccccct ccacaccctc
     1441 tttccccaac ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc
     1501 acccgtcggc acctccgctt caaggtacgc cgctcgtccy cccccccccc cccctctcta
     1561 ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac ttctgttcat
     1621 gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta cacggatgcg
     1681 acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt ggggaatcct
     1741 gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt gtttcgttgc
     1801 atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg
     1861 tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt
     1921 tctagatcgg agtagaatta attctgtttc aaactacctg gtggatttat taattttgga
     1981 tctgtatgtg tgtgccatac atattcatag ttacgaattg aagatgatgg atggaaatat
     2041 cgatctagga taggtataca tgttgatgcg ggttttactg atgcatatac agagatgctt
     2101 tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg tcgttcattc gttctagatc
     2161 ggagtagaat actgtttcaa actacctggt gtatttatta attttggaac tgtatgtgtg
     2221 tgtcatacat cttcatagtt acgagtttaa gatggatgga aatatcgatc taggataggt
     2281 atacatgttg atgtgggttt tactgatgca tatacatgat ggcatatgca gcatctattc
     2341 atatgctcta accttgagta cctatctatt ataataaaca agtatgtttt ataattattt
     2401 tgatcttgat atacttggat gatggcatat gcagcagcta tatgtggatt tttttagccc
     2461 tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtcgatgct caccctgttg
     2521 tttggtgtta cttctgcagg tcgactctag aggatcccct cgaggcgcgc caagctatca
     2581 aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
     2641 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
     2701 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat
     2761 gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag
     2821 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     2881 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
     2941 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
     3001 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg
     3061 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     3121 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     3181 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     3241 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     3301 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     3361 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     3421 agaatgctta atgaattaca acagtactgc gatgagtggc aggcggggcg taatctagag
     3481 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     3541 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     3601 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     3661 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     3721 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     3781 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     3841 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     3901 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga
     3961 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     4021 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     4081 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     4141 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     4201 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     4261 gttcagcttt cttgtacaaa gtggttcgat aattcttaat taactagttc tagagcggcc
     4321 gccaccgcgg tggagctcga attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt
     4381 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     4441 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     4501 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     4561 cgtattggct agagcagctt gccaacatgg tggagcacga cactctcgtc tactccaaga
     4621 atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
     4681 tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag
     4741 tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc
     4801 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
     4861 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgaa catggtggag
     4921 cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct
     4981 attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct
     5041 atctgtcact tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat
     5101 tgcgataaag gaaaggctat cgttcaagat gcctctgccg acagtggtcc caaagatgga
     5161 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
     5221 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     5281 caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg ctgaaatcac
     5341 cagtctctct ctacaaatct atctctctcg agtctaccat gagcccagaa cgacgcccgg
     5401 ccgacatccg ccgtgccacc gaggcggaca tgccggcggt ctgcaccatc gtcaaccact
     5461 acatcgagac aagcacggtc aacttccgta ccgagccgca ggaaccgcag gagtggacgg
     5521 acgacctcgt ccgtctgcgg gagcgctatc cctggctcgt cgccgaggtg gacggcgagg
     5581 tcgccggcat cgcctacgcg ggcccctgga aggcacgcaa cgcctacgac tggacggccg
     5641 agtcgaccgt gtacgtctcc ccccgccacc agcggacggg actgggctcc acgctctaca
     5701 cccacctgct gaagtccctg gaggcacagg gcttcaagag cgtggtcgct gtcatcgggc
     5761 tgcccaacga cccgagcgtg cgcatgcacg aggcgctcgg atatgccccc cgcggcatgc
     5821 tgcgggcggc cggcttcaag cacgggaact ggcatgacgt gggtttctgg cagctggact
     5881 tcagcctgcc ggtaccgccc cgtccggtcc tgcccgtcac cgagatttga ctcgagtttc
     5941 tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc
     6001 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
     6061 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat cccccgaatt
     6121 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
     6181 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
     6241 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
     6301 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
     6361 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
     6421 aacgatgaca gagcgttgct gcctgtgatc accgcggttt caaaatcggc tccgtcgata
     6481 ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt
     6541 tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta
     6601 tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc
     6661 ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc
     6721 tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag
     6781 ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga
     6841 aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct
     6901 gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc
     6961 tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc
     7021 ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa
     7081 taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc
     7141 gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca
     7201 cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga
     7261 tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat
     7321 cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa
     7381 gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga
     7441 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7501 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7561 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7621 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     7681 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7741 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7801 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7861 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7921 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7981 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8041 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8101 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8161 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8221 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8281 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     8341 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     8401 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     8461 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     8521 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc
     8581 ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt
     8641 gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc
     8701 ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt
     8761 gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa
     8821 agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga
     8881 ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg
     8941 ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc
     9001 tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc
     9061 ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt
     9121 caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt
     9181 cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc
     9241 tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat
     9301 caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg
     9361 gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac
     9421 gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt
     9481 ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac
     9541 caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac
     9601 gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga
     9661 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
     9721 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
     9781 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
     9841 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
     9901 cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg
     9961 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
    10021 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
    10081 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
    10141 ccattgcagg gccggcaggc aacccagccg cttacgcctg gccaaccgcc cgttcctcca
    10201 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
    10261 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
    10321 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
    10381 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
    10441 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
    10501 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
    10561 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
    10621 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
    10681 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
    10741 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
    10801 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
    10861 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
    10921 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
    10981 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
    11041 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
    11101 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
    11161 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
    11221 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
    11281 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
    11341 ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg
    11401 cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt
    11461 gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc
    11521 ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc
    11581 gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc
    11641 tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt
    11701 gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg
    11761 ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac
    11821 catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc
    11881 gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg
    11941 gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag
    12001 ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc
    12061 cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata
    12121 gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca
    12181 agtatt
//
