LOCUS       sequence-261005-        4863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC7A14
FEATURES             Location/Qualifiers
     source          1..4863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2988..3087)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3104..3123)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3105..3123)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3128..3144)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3128..3144)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3246..3265)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3257..4066
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3324..3343)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4213..4801
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4702..4721
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgtctg gcttctttac aagcctggac ccaaggagag tgcagtgggg
      721 agcagcatgg tacgcaatgc actcccggat cctgagaacc aagcctgtgg agtctatgct
      781 ggagggaaca ggaaccacaa ccgcacacgg aaccaagctg gcacaggtgc tgacaaccgt
      841 ggatctgatc tccctgggag tgggatcttg cgtgggaacc ggcatgtatg tggtgagcgg
      901 actggtggca aaggagatgg caggaccagg cgtgatcgtg tccttcatca tcgcagcagt
      961 ggcatccatc ctgtctggcg tgtgctacgc cgagtttggc gtgcgggtgc ctaagacaac
     1021 cggcagcgcc tacacatatt cctacgtgac cgtgggcgag ttcgtggcct tctttatcgg
     1081 ctggaacctg atcctggagt atctgatcgg aacagcagca ggagcatccg ccctgagctc
     1141 catgtttgac tctctggcca accacacaat ctccagatgg atggccgatt ctgtgggcac
     1201 cctgaatggc ctgggcaagg gcgaggagag ctaccctgac ctgctggccc tgctgatcgc
     1261 cgtgatcgtg accatcatcg tggccctggg cgtgaagaac tccatcggct tcaacaatgt
     1321 gctgaacgtg ctgaatctgg ccgtgtgggt gtttatcatg atcgccggcc tgttctttat
     1381 caatggcaag tattgggcag agggacagtt cctgccacac ggatggagcg gcgtgctgca
     1441 gggagcagca acctgcttct acgcctttat cggcttcgac atcatcgcca caaccggcga
     1501 ggaggccaag aaccctaata caagcatccc atatgccatc accgcctccc tggtcatctg
     1561 tctgacagcc tacgtgagcg tgtccgtgat cctgaccctg atggtgccat actatacaat
     1621 cgataccgag tcccccctga tggagatgtt tgtggcccac ggcttctacg ccgccaagtt
     1681 tgtggtggca atcggatctg tggcaggact gaccgtgagc ctgctgggat ccctgttccc
     1741 catgcctaga gtgatctacg caatggcagg cgacggactg ctgttcagat ttctggccca
     1801 cgtgtctagc tacacagaga ccccagtggt ggcctgcatc gtgagcggct ttctggccgc
     1861 cctgctggcc ctgctggtgt ctctgaggga tctgatcgag atgatgagca tcggcacact
     1921 gctggcctat accctggtga gcgtgtgcgt gctgctgctg cgctaccagc ccgagagcga
     1981 catcgatggc tttgtgaagt tcctgtccga ggagcacacc aagaagaagg agggcatcct
     2041 ggccgactgc gagaaggagg catgtagccc cgtgagcgag ggcgatgagt tctccggacc
     2101 agcaacaaac acctgcggag ccaagaatct gccatctctg ggcgacaatg agatgctgat
     2161 cggcaagtct gataagagca catataacgt gaatcacccc aactacggca ccgtggacat
     2221 gacaaccggc atcgaggccg atgagagcga gaatatctat ctgatcaagc tgaagaagct
     2281 gatcggcccc cactactata caatgaggat caggctggga ctgccaggca agatggacag
     2341 gcctacagca gcaaccggac acacagtgac catctgcgtg ctgctgctgt tcatcctgat
     2401 gttcatcttt tgtagcttta tcatcttcgg ctccgattac atctccgagc agtcttggtg
     2461 ggccatcctg ctggtggtgc tgatggtgct gctgatctcc accctggtgt ttgtgatcct
     2521 gcagcagcca gagaacccca agaagctgcc ttatatggcc ccttgcctgc catttgtgcc
     2581 cgccttcgcc atgctggtga atatctacct gatgctgaag ctgagcacaa tcacctggat
     2641 caggttcgcc gtgtggtgtt ttgtgggcct gctgatctat ttcggctacg gcatctggaa
     2701 ctctacactg gagatcagcg ccagggagga ggccctgcac cagtccacct atcagcggta
     2761 cgacgtggac gatcctttct ctgtggagga gggctttagc tatgccacag agggagagtc
     2821 tcaggaggac tggggaggac caaccgagga taagggcttt tactatcagc agatgagcga
     2881 tgccaaggcc aatggccgca cctcctctaa ggccaagtct aagagcaagc acaagcagaa
     2941 ctctgaggcc ctgatcgcca atgacgagct ggattacagc cccgaggacc cagctttctt
     3001 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     3061 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     3121 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     3181 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     3241 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     3301 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     3361 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     3421 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     3481 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     3541 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     3601 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     3661 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     3721 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     3781 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     3841 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     3901 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3961 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     4021 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     4081 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     4141 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     4201 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4261 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4321 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4381 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4441 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4501 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4561 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4621 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4681 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4741 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4801 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4861 gtt
//
