LOCUS       sequence-26101-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Antonellis A, Huynh JL, Lee-Lin SQ, Vinton RM, Renaud G, Loftus SK, 
            Elliot G, Wolfsberg TG, Green ED, McCallion AS, Pavan WJ
  TITLE     Identification of neural crest and glial enhancers at the mouse 
            Sox10 locus through transgenesis in zebrafish.
  JOURNAL   PLoS Genet. 2008  . 4(9):e1000174.
  PUBMED    18773071
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Genet.
            2008  . 4(9):e1000174."
COMMENT     Sequence Label: pE1B-C-F/Sox10-MCS7
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    135..159
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(459..483)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    521..612
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(553..572)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    626..674
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgg tggctttacc aacagtaccg
       61 gaatgccaag cttaagatga actattcaaa gcaacaccaa cccagcttta tatccctgag
      121 atctcgagcc catcacaagt ttgtacaaaa aagcaggctg tggcctcagg cccctgtgcc
      181 cgcgcccatg gggttaaatc tctccctgtc tctctctgct ccaagttgtt ttccaagcga
      241 ggcagagaat ggttctcttg ttaccaacat ggcttctggg cattgggtaa tgcgttccct
      301 cttctccctg ccctgcgcca caaagggacc ttttgttcct ctcccggccc ctctctctgg
      361 ctcttctctg ccccccaccc acctcaagtc cctcctcccc acgcctcctg ccctactctg
      421 cgcgcacccc tgggctgtga ggagtgaccg tggcctggac ccagctttct tgtacaaagt
      481 ggtgatggct agcacgcgta agagctcggt acctatcgat agagaaatgt tctggcacct
      541 gcacttgcac tggggacagc ctattttgct agtttgtttt gtttcgtttt gttttgatgg
      601 agagcgtatg ttagtactat cgattcacac aaaaaaccaa cacacagatg taatgaaaat
      661 aaagatattt tattgcggcc gctccaagta cctcccgtac cttaatatta cttacttatc
      721 atggtagctt gggctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      781 tgcgcagcct gaatggcgaa tggcaaattg
//
