LOCUS       sequence-261012-        4842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC26A3
FEATURES             Location/Qualifiers
     source          1..4842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2967..3066)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3083..3102)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3084..3102)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3107..3123)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3107..3123)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3225..3244)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3236..4045
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3303..3322)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4192..4780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4681..4700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgatcg agcccttcgg caaccagtac atcgtggccc ggcccgtgta
      721 ctctaccaac gccttcgagg agaatcacaa gaagaccggc agacaccaca agacatttct
      781 ggatcacctg aaggtgtgct gtagctgctc ccctcagaag gccaagagga tcgtgctgtc
      841 cctgttccct atcgcctctt ggctgccagc ctaccgcctg aaggagtggc tgctgagcga
      901 tatcgtgtct ggcatcagca ccggaatcgt ggccgtgctg cagggactgg cctttgccct
      961 gctggtggac atcccccccg tgtacggcct gtatgcctcc ttctttccag ccatcatcta
     1021 tctgttcttt ggcacatccc ggcacatctc tgtgggccct ttcccaatcc tgtctatgat
     1081 ggtgggactg gccgtgagcg gagccgtgtc caaggccgtg ccagacagaa atgccaccac
     1141 actgggcctg cccaacaata gcaacaatag ctccctgctg gacgatgaga gggtgcgcgt
     1201 ggcagcagca gcctctgtga ccgtgctgag cggcatcatc cagctggcct tcggcatcct
     1261 gcggatcggc tttgtggtca tctacctgtc tgagagcctg atctccggct ttaccacagc
     1321 agcagcagtg cacgtgctgg tgtctcagct gaagttcatc tttcagctga ccgtgccaag
     1381 ccacacagat cccgtgtcca tcttcaaggt gctgtattcc gtgttttctc agatcgagaa
     1441 gaccaacatc gccgacctgg tgacagccct gatcgtgctg ctggtggtga gcatcgtgaa
     1501 ggagatcaat cagaggttca aggacaagct gcccgtgcct atcccaatcg agtttatcat
     1561 gaccgtgatc gcagcaggcg tgtcctacgg ctgcgatttc aagaaccgct ttaaggtggc
     1621 cgtggtgggc gacatgaatc ctggcttcca gccacccatc acaccagatg tggagacctt
     1681 ccagaacaca gtgggcgact gttttggaat cgcaatggtg gccttcgccg tggcctttag
     1741 cgtggcctcc gtgtattctc tgaagtacga ttatcctctg gacggcaacc aggagctgat
     1801 cgccctgggc ctgggcaata tcgtgtgcgg cgtgttccgg ggatttgcag gatctaccgc
     1861 cctgtccaga tctgccgtgc aggagagcac cggaggcaag acacagatcg caggactgat
     1921 cggcgccatc atcgtgctga tcgtggtgct ggcaatcggc ttcctgctgg cacccctgca
     1981 gaagtccgtg ctggccgccc tggccctggg aaacctgaag ggcatgctga tgcagtttgc
     2041 cgagatcggc cggctgtgga gaaaggataa gtacgactgt ctgatctgga tcatgacctt
     2101 catctttaca atcgtgctgg gactgggact gggactggca gccagcgtgg cctttcagct
     2161 gctgaccatc gtgttccgga cacagtttcc taagtgctcc accctggcca atatcggcag
     2221 aacaaacatc tacaagaata agaaggatta ctacgacatg tacgagccag agggcgtgaa
     2281 gatcttcagg tgtccctctc ctatctactt tgccaacatc ggcttctttc ggagaaagct
     2341 gatcgatgcc gtgggcttca gcccactgcg catcctgcgg aagagaaata aggccctgag
     2401 gaagatccgc aagctgcaga agcagggact gctgcaggtg accccaaagg gctttatctg
     2461 taccgtggac acaatcaagg attccgacga ggagctggat aacaatcaga tcgaggtgct
     2521 ggaccagcca atcaacacca cagatctgcc cttccacatc gactggaacg acgatctgcc
     2581 cctgaatatc gaggtgccta agatcagcct gcactccctg atcctggatt tctctgccgt
     2641 gagctttctg gacgtgtcta gcgtgagggg cctgaagagc atcctgcagg agttcatccg
     2701 catcaaggtg gacgtgtaca tcgtgggcac cgacgatgac tttatcgaga agctgaatag
     2761 atatgagttc tttgacggcg aggtgaagtc ctctatcttc tttctgacca tccacgatgc
     2821 cgtgctgcac atcctgatga agaaggacta cagcacatcc aagttcaacc cctcccagga
     2881 gaaggatggc aagatcgact ttaccatcaa cacaaatggc ggcctgagga atcgcgtgta
     2941 tgaggtgcct gtggagacaa agttcgaccc agctttcttg tacaaagttg gcattataag
     3001 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     3061 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     3121 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     3181 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     3241 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     3301 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     3361 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     3421 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     3481 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
     3541 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     3601 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     3661 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     3721 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     3781 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     3841 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     3901 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     3961 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     4021 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     4081 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta
     4141 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4201 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4261 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4321 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4381 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4441 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4501 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4561 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4621 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4681 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4741 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4801 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
